Quantitative estimation of porcine endogenous retrovirus release from PK15 cells

• Autorzy: Kimsa M. , Strzalka-Mrozik B. , Kimsa M. , Adamska J. , Gola J. , Lopata K. , Mazurek U.
• Języki publikacji: EN

Abstrakty

EN

The present study focuses on the assessment of porcine endogenous retrovirus (PERV) release from PK15 cells in a time dependent manner. the highest amount of PERV A RNA was detected in PK15 cells after 16 hours of culture. !e highest amount of PERV B RNA was detected in PK15 cells after 20 hours. !e highest amount of both subtypes RNAs was detected in culture medium after 32 hours of culture. the peaks of PERV reverse transcriptase (RT) activity were detected after 28 h of culture in PK15 cells and after 32 hours in the culture medium. the monitoring of PERV release from PK15 cell line may be useful for the evaluation of PERV replication.

Słowa kluczowe

EN

quantitative estimation; porcine endogenous retrovirus; PK15 cell culture; prevalence; real-time polymerase chain reaction; culture medium

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 3
• Opis fizyczny: p.211-215,fig.,ref.

Twórcy

autor

Kimsa M.

• Department of Molecular Biology, Medical University of Silesia, Narcyzow 1, 41-200 Sosnowiec, Poland

autor

Strzalka-Mrozik B.

autor

Kimsa M.

autor

Adamska J.

autor

Gola J.

autor

Lopata K.

autor

Mazurek U.

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