Lateral thoracic hemisection of the spinal cord in adult rats results in initial severe impairment of hindlimb movements followed by a relatively fast improvement of locomotor functions. There are data showing substantial or even complete recovery of locomotor performance within 21 – 30 days after spinal cord injury. In our previous study we showed that improvement of locomotor performance reached plateau about four weeks after the injury but animals did not show the full locomotor recovery. The aim of present study was to analyze the time course of relationships between activity of flexors and extensors of each of four limbs during locomotion after lateral hemisection of the spinal cord in rats. The locomotion was tested in freely moving animals walking at speed 0.4 – 1.0 m/s. Bipolar EMG electrodes for the chronic recordings were implanted in soleus and tibialis muscles of hindlimbs and biceps and triceps of forelimbs. The EMG recordings were performed once a week up to six weeks after spinal cord injury. Our results showed that 7 days after the lateral hemisection of the spinal cord the relationship between flexor and extensor muscle (intralimb coordination) of hindlimbs was severely impaired. Moreover, analysis of interlimb coordination revealed that relationships between forelimbs and hindlimbs and hindlimbs themselves were also impaired. Two weeks after surgery the relationships between flexor and extensor muscle of right as well as left hindlimb returned to normal. Diagonal coordination between left forelimb and right hindlimb was also similar to that before the lesion. Only coordination between left hindlimb and remaining three limbs was impaired through the whole period of the study (six weeks after surgery). This results confirmed hypothesis, that after lateral thoracic hemisection of the spinal cord rats did not show the full locomotor recovery.