Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 37 | 01 |
Tytuł artykułu

Alteration in anti-oxidant defense system and protein expression in response to varied concentrations of EMS in Psoralea corylifolia

Treść / Zawartość
Warianty tytułu
Języki publikacji
Germplasm of Psoralea corylifolia L.,one of the most important endangered medicinal plants of Chinese and Indian Pharmacopeia were treated with ethyl methane sulphonate (0, 0.1, 0.25, 0.50, 0.75 and 1 %) and sampled for observations in the form of growth, non enzymatic systems and stress proteins at pre-and post-flowering stages of development. Higher concentrations of EMS showed significant (P\0.001) inhibition in the germination percentage from 3.88 to 35.46 % at pre-flowering stage and from 4.90 to 35.97 % at post-flowering stage when compared to control. Other growth related parameters viz shoot growth and root growth also declined within increase in the dose as compared to control plants. Root growth was severely affected as compared to shoot growth. Roots showed a significant correlation with tolerance index as increasig concentration of EMS progressively inhibited the root growth. Plants developed from treated germplasm at higher doses exhibit higher activity of ascorbate peroxidase (APX), up to flowering and after that declined. Contrast to that, total soluble protein (TSP) content decreased in age and dose wise manner. The EMS dose applied and oxidative stress was correlated and derived from the increased antioxidant activities and decrease in total glutathione content from 122.75 % (GSH) and 103.29 % (GSSG) in plants raised from treated germplasm towards the post-flowering stage. Pronounced accumulation of psoralen from 10.74 to 93.24 % was observed following higher doses of EMS at post-flowering stage, where EMS induced oxidative stressenhanced proline accumulation and lipid peroxidation. In addition, the prevalence of maximum antioxidant activity, psoralen accumulation was an efficient process in acclimatizing P. corylifolia to stress. 2-Dimensional gel electrophoresis (2D-GE) of the protein expression profile and MALDI TOF–MS revealed an expression of 20 different proteins which are associated with such functions as cellular detoxification, stress defense, psoralen production, photosynthesis, signal transduction, and cell cycle. The increase in the psoralen pool after EMS treatment proved to be a best protective antioxidant to counterbalance EMS-induced ROS detoxification and can be used as a biomarker and a scavenger in stress physiology.
Słowa kluczowe
Opis fizyczny
Article: 1707 [13 p.], fig.,ref.
  • Cell Biology, Molecular Biology and Genetics Section, Department of Botany, Aligarh Muslim University, Aligarh 20202, India
  • Cell Biology, Molecular Biology and Genetics Section, Department of Botany, Aligarh Muslim University, Aligarh 20202, India
  • Cell Biology, Molecular Biology and Genetics Section, Department of Botany, Aligarh Muslim University, Aligarh 20202, India
  • Cell Biology, Molecular Biology and Genetics Section, Department of Botany, Aligarh Muslim University, Aligarh 20202, India
  • Department of Biochemistry, Aligarh Muslim University, Aligarh 20202, India
  • Abdelmohsen K, Gorospe M (2012) RNA-binding protein nucleolin in disease RNA. RNA Biol 9(6):799–808
  • Ali GM, Komatsu S (2006) Proteomic analysis of rice leaf sheath during drought stress. J Prot Res 5(2):396–403
  • Ali J, Akhtar N, Sultana Y, Baboota S, Ahuja A (2008a) Antipsoriatic microemulsion gel formulations for topical drug delivery of babchi oil (Psoralea corylifolia). Method Find Exp Clin Pharmacol 30:277–285
  • Ali ST, Mahmooduzzafar Abdin MZ, Iqbal M (2008b) Ontogenic changes in foliar and psoralen content of Psoralea corylifolia Linn. exposed to SO2 stress. J Environ Biol 29:661–668
  • Alia M, Ramos S, Mateos R, Granado-Serrano AB, Bravo L, Goya L (2006) Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide. Toxicol Appl Pharmacol 212(2):110–118
  • Anjum NA, Umar S, Chan M-T (eds) (2010) Ascorbate glutathione pathway and stress tolerance in plants. Springer, Berlin
  • Anjum NA, Ahmad I, Mohmood I, Pacheco M, Duarte AC, Pereira E, Prasad MNV (2012) Modulation of glutathione and its related enzymes in plants responses to toxic metals and metalloids—a review. Environ Exp Bot 75:307–324
  • Azevedo L, Alves de Lima PL, Gomes JC, Stringheta PC, Ribeiro DA, Salvadori DM (2007) Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo. Food Chem Toxicol 45(5):852–858
  • Badowiec A, Weidner S (2014) Proteomic changes in the roots of germinating Phaseolus vulgaris seeds in response to chilling stress and post-stress recovery. J Plant Physiol 171(6):389–398
  • Baszynski T, Krupa Z (1995) Some aspects of heavy metals toxicity towards photosynthetic apparatus-direct and indirect effects on light and dark reactions. Acta Physiol Plant 17(2):177–190
  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207
  • Bhat TM, Ansari MYK, Aslam R (2012) Sodium azide (NaN3) induced genetic variation of Psoralea corylifolia L. and analysis of variants using RAPD markers. Nucleus 55(3):149–154
  • Bolwell GP, Bindschedler LV, Blee KA, Butt VS, Davies DR, Gardner SL, Minibayeva F (2002) The apoplastic oxidative burst in response to biotic stress in plants: a three-component system. J Exp Bot 53(372):1367–1376
  • Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S, Pommier Y (2008) H2AX and cancer. Nat Rev Cancer 8(12):957–967
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1):248–254
  • Bradley DJ, Kjellbom P, Lamb CJ (1992) Elicitor- and wound induced oxidase cross-linking of a plant cell wall proline-rich protein: a novel, rapid defense response. Cell 70:21–30
  • Calabrese Edward J, Bachmann KA, Bailer AJ, Bolger PM, Borak J, Cai L, Cedergreen N et al (2007) Biological stress response terminology: integrating the concepts of adaptive response and preconditioning stress within a hormetic dose–response framework. Toxicol Appl Pharmacol 222(1):122–128
  • Chen X, Schluesener HJ (2008) Nanosilver: a nanoproduct in medical application. Toxicol Lett 176(1):1–12
  • Dannenberg AJ, Altorki NK, Boyle JO, Dang C, Howe LR,Weksler BB, Subbaramaiah K (2001) Cyclo-oxygenase 2: a pharmacological target for the prevention of cancer. Lancet Oncol 2(9):544–551
  • Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Holzer R, Feller U (2004) Biochemical changes in barley plants after excessive supply of copper and manganese. Environ Exp Bot 52(3):253–266
  • Dercks W, Trumble JT, Winter C (1990) Impact of atmospheric pollution on linear, furanocoumarin content in celery. J Chem Ecol 16:443–454
  • Dimova EY, Kietzmann T (2008) Metabolic, hormonal and environmental regulation of plasminogen activator inhibitor-1 (PAI-1) expression: lessons from the liver. Thromb Haemost 100(6):992–1006
  • Droge W (2002) Aging-related changes in the thiol/disulfide redox state: implications for the use of thiol antioxidants. Exp Gerontol 37(12):1333–1345
  • Dubovskaya LV, Kolesneva EV, Knyazev DM, Volotovskii ID (2007) Protective role of nitric oxide during hydrogen peroxideinduced oxidative stress in tobacco plants. Rus J Plant Physiol 54(6):755–762
  • Foyer CH, Souriau N, Perret S, Lelandais M, Kunert KJ, Pruvost C, Jouanin L (1995) Overexpression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees. Plant Physiol 109(3):1047–1057
  • Gao L, Mann GE (2009) Vascular NADPH oxidase activation in diabetes: a double-edged sword in redox signalling. Cardiovasc Res 82(1):9–20
  • Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141(2):341–345
  • Gill SS, Tuteja N (2010a) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48(12):909–930
  • Gill SS, Tuteja N (2010b) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48(12):909–930
  • Gill SS, Khan NA, Tuteja N (2012) Cadmium at high dose perturbs growth, photosynthesis and nitrogen metabolism while at low dose it up regulates sulfur assimilation and antioxidant machinery in garden cress (Lepidium sativum L). Plant Sci 182:112–120
  • Hajduch E, Litherland GJ, Hundal HS (2001) Protein kinase B (PKB/Akt)—a key regulator of glucose transport? FEBS Lett 492(3):199–203
  • Hasanuzzaman M, Hossain MA, Fujita M (2012) Exogenous selenium pretreatment protects rapeseed seedlings from cadmium-induced oxidative stress by up-regulating antioxidant defense and methylglyoxal detoxification systems. Biol Trace Elem Res 149(2):248–261
  • Houtz RL, Nable RO, Cheniae GM (1988) Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. Plant Physiol 86(4):1143–1149
  • Innocenti G, Dall’Acqua F, Guiotto A, Caporale G (1977) Investigation on skin-photosentizing activity of various kinds of Psoralea. Plant Med 31(02):151–155
  • Jan S, Parween T, Siddiqi TO (2011) Gamma radiation effects on growth and yield attributes of Psoralea corylifolia L with reference to enhanced production of Psoralen. Plant Growth Regul 64(2):163–171
  • Jan S, Parween T, Siddiqi TO (2012a) Anti-oxidant modulation in response to gamma radiation induced oxidative stress in developing seedlings of Psoralea corylifolia. J Environ Radioact 113:142–149
  • Jan S, Parween T, Siddiqi TO, Mahmooduzaffar N (2012b) Enhancement in furanocoumarin content and phenylalanine ammonia lyase activity in developing seedlings of Psoralea corylifolia L. in response to gamma irradiation of seeds. Radiat Environ Biophys 51(3):341–347
  • Karpinski S, Escobar C, Karpinska B, Creissen G, Mullineaux PM (1997) Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. Plant Cell 9(4):627–640
  • Kim CY, Liu Y, Thorne ET, Yang H, Fukushige H, Gassmann W, Hildebrand D, Sharp RE, Zhang S (2003) Activation of a stress-responsive mitogen-activated protein kinase cascade induces the biosynthesis of ethylene in plants. Plant cell 15(11):2707–2718
  • Klessig, Daniel F, Durner J, Noad R, Duroy A Navarre, Wendehenne D, Kumar D, Jun Ma Zhou et al (2000) Nitric oxide and salicylic acid signaling in plant defense. Proceedings of the National Academy of Science 97 no 16:8849–8855
  • Koistinen R, Kalkkinen N, Huhtala ML, Seppala M, Bohn H, Rutanen EM (1986) Placental protein 12 is a decidual protein that binds somatomedin and has an identical N-terminal amino acid sequence with sdmatomedin-binding protein from human amhiotic fluid. Endocrinol 118(4):1375–1378
  • Krishnamurthy AK (1969) The wealth of India: raw materials, vol VII. CSIR, New Delhi, pp 295–298
  • Krizek DT, Mirecki RM, Britz SJ (1997) Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cucumber. Physiologia Plantarum 100(4):886–893
  • Kumar A, Pandey AK, Singh SS, Shanker R, Dhawan A (2011) Engineered ZnO and TiO2 sub nanoparticles induce oxidative stress and DNA damage leading to reduced viability of Escherichia coli. Free Radic Biol Med 51(10):1872–1881
  • Laval F (1988) Pretreatment with oxygen species increases the resistance of mammalian cells to hydrogen peroxide and c-rays. Mutat Res/Fundam Mol Mech Mutat 201(1):73–79
  • Mann ME, Zhang Z, Rutherford S, Bradley RS, Hughes MK, Shindell D, Ni F (2009) Global signatures and dynamical origins of the little ice age and medieval climate. Anom Sci 326(5957):1256–1260
  • Mazher AA, Zaghloul SM, Mahmoud SA, Siam HS (2011) Stimulatory effect of kinetin, ascorbic acid and glutamic acid on growth and chemical constituents of Codiaeum variegatum L plants. Am Eurasian J Agric Environ Sci 10:318–323
  • McDaniel KL, Toman FR (1994) Short-term effects of manganese toxicity on ribulose 1, 5 bisphosphate carboxylase in tobacco chloroplasts. J Plant Nut 17(2–3):523–536
  • Mehta SK, Gaur JP (1999) Heavy-metal-induced proline accumulation and its role in ameliorating metal toxicity in Chlorella vulgaris. New Phytol 143(2):253–259
  • Misra P, Pandey A, Tewari SK, Nath P, Trivedi PK (2010) Characterization of isoflavone synthase gene from Psoralea corylifolia: a medicinal plant. Plant Cell Rep 29(7):747–755
  • Moghaddam SS, Jafar H, Ibrahim R, Rahmat A, Aziz MA, Philip E (2011) Effect of acute gamma irradiation on physiological traits and flavanoid accumulation of Centella asiatica. Molecules 16:4994–5007
  • Montero J, Dutta C, Van Bodegom D, Weinstock D, Letai A (2013) p53 regulates a non-apoptotic death induced by ROS. Cell Death Differ 20(11):1465–1474
  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880
  • Noctor G, Queval G, Mhamdi A, Chaouch S, Foyer CH, Glutathione (2011) The Arabidopsis Book. American Society of Plant Biologists, vol 9
  • Pang CH, Wang BS (2010) Role of ascorbate peroxidase and glutathione reductase in ascorbate–glutathione cycle and stress tolerance in plants In: ascorbate-glutathione pathway and stress tolerance in plants. Springer, Netherlands, p 91–113
  • Panagopoulos I, Bornman JF, Bjo¨rn LO (1992) Response of sugar beet plants to ultraviolet-B (280–320 nm) radiation and Cercospom leaf spot disease. Physiologia Plantarum 84(1):140–145
  • Rauser WE (1995) Phytochelatins and related peptides structure, biosynthesis, and function. Plant Physiol 109(4):1141
  • Reitz SR, Karowe DN, Diawara MM, Tumble JT (1997) Effects of elevated atmospheric carbon dioxide on the growth and linear furanocoumarins content of celery. J Agric Food Chem 45:3642–3646
  • Rieger R, Michaelis A, Takehisa S (1990) On adaptive responses of plant meristem cells in vivo: Protection against induction of chromatid aberration. In: Obe G, Nataraja AT (eds) Chromosomal aberrations: basic and applied aspects. Springer, Berlin, pp 163–179
  • Rimbach G, Boesch-Saadatmandi C, Frank J, Fuchs D, Wenzel U, Daniel H, Weinberg PD (2008) Dietary isoflavones in the prevention of cardiovascular disease—a molecular perspective. Food Chem Toxicol 46(4):1308–1319
  • Roy H (1989) Rubisco assembly: a model system for studying the mechanism of chaperonin action. Plant Cell 1(11):1035
  • Ruan B, Kong LY, Takaya Y, Niwa M (2007) Studies on the chemical constituents of Psoralea corylifolia L. J Asian Nat Prod Res 9:41–44
  • Salvucci ME, Crafts-Brandner SJ (2004) Inhibition of photosynthesis by heat stress: the activation state of Rubisco as a limiting factor in photosynthesis. Physiol Plant 120(2):179–186
  • Scott SL (1996) The Cray T3E network: adaptive routing in a high performance 3D torus
  • Siddiqui SA, Ansari MYK (1995) Mutagenic effects of ethyl methane sulphonate on Ammi majus L. 1. morphological variations, mutation frequency and yield. Phytomorphology 45:23–29
  • Siow R, Mann GE (2010) Dietary isoflavones and vascular protection: activation of cellular antioxidant defenses by SERMs or hormesis. Mol Asp Med 31(6):468–477
  • Uikey SK, Yadav AS, Sharma AK, Rai AK, Raghuwanshi DK, Badkhane Y (2010) The botany, chemistry, pharmacological and therapeutic application of Psoralea corylifolia L.—a review. Int J Phytomedicine 2:100–107
  • Verma K, Shekhawat GS, Sharma A, Mehta SK, Sharma V (2008) Cadmium induced oxidative stress and changes in soluble and ionically bound cell wall peroxidase activities in roots of seedling and 3–4 leaf stage plants of Brassica juncea (L) czern. Plant Cell Rep 27(7):1261–1269
  • Wand D, Famei H, Jiang Z (2001) Osteoblastic proliferation stimulation activity of Psoralea corylifolia extracts and two of its flavonoids. Int Immunopharmacol 1:1849–1855
  • Yamane H, Ikeda K, Ushijima K, Sassa H, Tao R (2003) A pollenexpressed gene for a novel protein with an F-box motif that is very tightly linked to a gene for S-RNase in two species of cherry, Prunus cerasus and P avium. Plant Cell Physiol 44(7):764–769
  • Zamir D (2001) Improving plant breeding with exotic genetic libraries. Nat Rev Genet 2(12):983–989
  • Zobel AM, Wang J, March RE, Brown SA (1991) Identification of eight coumarins occurring with psoralen, xanthotoxin, and bergapten on leaf surfaces. J Chem Ecol 17(9):1859–1870
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.