Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 32 | 6 |

Tytuł artykułu

Nitric oxide affecting root growth, lignification and related enzymes in soybean seedlings

Warianty tytułu

Języki publikacji



This study analyzed the involvement of nitric oxide (NO) in the root lignification of soybean seedlings. To this end, changes in root cell viability; phenylalanine ammonia-lyase (PAL) and soluble and cell wall bound peroxidase (POD) activities and lignin and hydrogen peroxide (H₂O₂) contents of soybean roots treated with the NO-donor sodium nitroprusside (SNP) and its relationships with root growth were evaluated. Seedlings were cultivated in a nutrient solution supplemented with 5 to 1,000 μM SNP for 24 h. At an extremely low concentration (5 μM), SNP induced root growth and increased lignification and activities of related enzymes (PAL and cell wall-bound POD). At a high concentration (1,000 μM), SNP reduced root growth and lignification (PAL activity and H₂O₂ and lignin contents) and caused a loss of cell viability. Application of potassium ferrocyanide (an analog of SNP that cannot release NO) and PTIO (2-phenyl-4,4,5,5,-tetramethylimidazoleline-1-oxyl-3-oxide, a scavenger of NO) revealed that the inhibitory/stimulatory effects on root lignification may be due to NO itself. These results indicate that NO, depending on its concentration, may act as a stress factor, due to its toxic action, or as a signal molecule, inducing soybean root growth and lignification.

Słowa kluczowe








Opis fizyczny



  • Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil
  • Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil
  • Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil
  • Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil
  • Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringa´, Av. Colombo, 5790, Maringa´, PR 87020-900, Brazil


  • Alexieva V, Sergiev I, Mapelli A, Karanov E (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344
  • Arasimowicz A, Floryszak-Wieczorek T (2007) Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Sci 172:876–887
  • Baker CJ, Mock NM (1994) An improved method for monitoring cell death in cell suspension and leaf discs assays using Evans blue. Plant Cell Tissue Organ Cult 39:7–12
  • Beligni MV, Lamattina L (2000) Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta 210:215–221
  • Boerjan W, Ralph J, Baucher M (2003) Lignin biosynthesis. Ann Rev Plant Biol 54:519–546
  • Bolwell GP, Cramer CL, Lamb CJ, Schuch W, Dixon RA (1986) L-Phenylalanine ammonia-lyase from Phaseolus vulgaris: modulation of the levels of active enzyme by trans-cinnamic acid. Planta 169:97–107
  • Clarke A, Desikan R, Hurst RD, Hancock JT, Neill SJ (2000) NO way back: nitric oxide and programmed cell death in Arabidopsis thaliana suspension cultures. Plant J 24:1–13
  • Correa-Aragunde N, Graziano M, Chevalier C, Lamattina L (2006) Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato. J Exp Bot 57:581–588
  • Delledonne M, Xia Y, Dixon RA, Lamb C (1998) Nitric oxide functions as a signal in plant disease resistance. Nature 394:585–588
  • Delledonne M, Polverari A, Murgia I (2003) The functions of nitric oxide-mediated signaling and changes in gene expression during the hypersensitive response. Antioxid Redox Signal 5:33–41
  • dos Santos W, Ferrarese MLL, Finger A, Teixeira ACN, Ferrarese-Filho O (2004) Lignification and related enzymes in Glycine max root growth-inhibition by ferulic acid. J Chem Ecol 30:1199–1208
  • Enkhardt U, Pommer U (2000) Influence of nitric oxide and nitrite on the activity of cinnamic acid 4-hydroxylase of Zea mays in vitro. J Appl Bot 74:151–154
  • Ferrarese MLL, Ferrarese-Filho O, Rodrigues JD (2000a) Ferulic acid uptake by soybean root in nutrient culture. Acta Physiol Plant 22:121–124
  • Ferrarese MLL, Rodrigues JD, Ferrarese-Filho O (2000b) Phenylalanine ammonia-lyase activity in soybean roots extract measured by reverse-phase high performance liquid chromatography. Plant Biol 2:152–153
  • Ferrarese MLL, Zottis A, Ferrarese-Filho O (2002) Protein-free lignin quantitation in soybean (Glycine max (L.) Merr.) roots. Biologia 57:541–543
  • Ferrer MA, Ros Barceló A (1999) Differential effects of nitric oxide on peroxidase and H₂O₂ production by the xylem of Zinnia elegans. Plant Cell Environ 22:891–897
  • Floryszak-Wieczorek J, Milczarek G, Arasimowicz M, Ciszewski A (2006) Do nitric oxide donors mimic endogenous NO-related response in plants? Planta 224:1363–1372
  • Gabaldón C, Ros LVG, Pedreño MA, Ros-Barceló A (2005) Nitric oxide production by the differentiating xylem of Zinnia elegans. New Phytol 165:5–7
  • Gazaryan IG, Lagrimini LM (1996) Purification and unusual kinetic properties of a tobacco anionic peroxidase. Phytochem 41:1029–1034
  • Gouveia CMCP, Souza IF, Magalhães ACN, Martins IS (1997) NO-releasing substances that induce growth elongation in maize root segments. Plant Growth Regul 21:183–187
  • Guo P, Cao Y, Li Z, Zhao B (2004) Role of endogenous nitric oxide burst in the resistance of wheat to stripe rust. Plant Cell Environ 27:473–477
  • Hu X, Neill SJ, Tang Z, Cai W (2005) Nitric oxide mediates gravitropic bending in soybean roots. Plant Physiol 137:663–670
  • Kolodziejek I, Koziol-Lipinska J, Waleza M, Korczynski J, Mostowska A (2007) Aspects of programmed cell death during early senescence of barley leaves: possible role of nitric oxide. Protoplasma 232:97–108
  • Neill SJ, Barros R, Bright J, Desikan R, Hancock J, Harrison J, Morris P, Ribeiro D, Wilson I (2008) Nitric oxide, stomatal closure and abiotic stress. J Exp Bot 59:165–176
  • Pagnussat GC, Simontacchi M, Puntarulo S, Lamattina L (2002) Nitric oxide is required for root organogenesis. Plant Physiol 129:954–956
  • París R, Lamattina L, Casalongué CA (2007) Nitric oxide promotes the wound-healing response of potato leaflets. Plant Physiol Biochem 45:80–86
  • Passardi F, Cosio C, Penel C, Dunand C (2005) Peroxidases have more functions than a Swiss army knife. Plant Cell Rep 24:255–265
  • Ros Barceló A, Pomar F, Ferrer MA, Martinez P, Ballesta MC, Pedreño MA (2002) In situ characterization of a NO-sensitive peroxidase in the lignifying xylem of Zinnia elegans. Physiol Plant 114:33–40
  • Ros Barceló A, Gabaldón C, Pomar F (2004) Nitric oxide, peroxidase and lignification in higher plants. In: Magalhaes JR, Singh RP, Passos LP (eds) Nitric oxide signaling in higher plants. Studium Press, Houston, pp 277–308
  • Sang JR, Jiang MY, Lin F, Xu S, Zhang A, Tan M (2008) Nitric oxide reduces hydrogen peroxide accumulation involved in water stress-induced subcellular anti-oxidant defense in maize plants. J Integr Plant Biol 50:231–243
  • Tian X, Lei Y (2006) Nitric oxide treatment alleviates drought stress in wheat seedlings. Biol Plant 50:775–778
  • Veitch NC (2004) Horseradish peroxidase: a modern view of a classic enzyme. Phytochem 65:249–259
  • Víteček J, Wünschová A, Petřek J, Adam V, Kizek R, Havel L (2007) Cell death induced by sodium nitroprusside and hydrogen peroxide in Tobacco By-2 cell suspension. Biol Plant 51:472–479
  • Wang JW, Zheng LP, Wu JI, Tand RX (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15:351–358
  • Wojtaszek P (2000) Nitric oxide in plants. To NO or not to NO. Phytochem 54:1–4
  • Zottini M, Formentin E, Scattolin M, Carimi F, Schiavo FL, Terzi M (2002) Nitric oxide affects plant mitochondrial functionality in vivo. FEBS Lett 515:75–78

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.