Impact of the priobiotic on the presence of selected virulence genes and drug-resistance among Campylobacter coli isolated from piglets

• Autorzy: Selwet M. , Galbas M.
• Języki publikacji: EN

Abstrakty

EN

The investigations comprised 100 piglets of crossbreed Polish Landrace x Large White Polish breed. Faeces samples were collected on the 2nd d of piglets' life (control). On the 5th d of life of the piglets, probiotic paste was applied and 7 d later, faecal samples were collected again. The material included 100 isolates of Campylobacter sp. obtained from healthy piglets. All isolates were assigned to the Campylobacter coli species. The occurrence of virulence genes was determined by the PCR method. Drug- resistance of the obtained isolates was determined using diffusion tests and E-test strips. All isolates deriving from the control group piglets were found to contain the cαdF gene responsible for adhesion, as well as, gene flaA influencing motility of the examined bacteria. In piglets fed diets supplemented with probiotics, the cαdF gene occurred in 100% isolates and gene flαA - in 99% isolates. Campylobacter coli isolates obtained from piglets from the control group exhibited the highest resistance with respect to ciprofloxacin and enrofloxacin. The similar results were recorded in the case of isolates obtained after the probiotic application. The majority of the isolates generated α type haemolysis (91%-92%). No significant differences were recorded in the capability of generating haemolysis between isolates obtained before probiotic administration and the isolates obtained after the application of the experimental probiotic.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 4
• Opis fizyczny: p.507-511,ref.

Twórcy

autor

Selwet M.

• Department of General and Environmental Microbiology, Poznan University of Life Sciences, 60-656 Poznan

autor

Galbas M.

• Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, 60-632 Poznan

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