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2012 | 34 | 5 |

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Cloning and characterization of a type 1 metallothionein gene from the copper-tolerant plant Elsholtzia haichowensis

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EhMT1, a type 1 metallothionein (MT) gene, was cloned from Elsholtzia haichowensis, a Cu-tolerant plant. Typical of plant type 1MTs,EhMT1encodes a putative peptide of 74 amino acid residues containing cysteine-rich domains. Sequence comparisons with various databases revealed strong similarities at the nucleotide and amino acid levels between EhMT1 and the type 1 MT of Mimulus guttatus. EhMT1 transcriptionwas greater in the roots than in the leaves, and was markedly increased by treatments with Cu, H₂O₂, and heat shock. EhMT1-GUS was localized to the cytoplasm of onion epidermal cells. Escherichia coli cells expressing pET-30a-EhMT1 were more tolerant to Cu and accumulated more Cu than control cells. Our results show that EhMT1 is involved in Cu tolerance and accumulation in E. haichowensis.

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  • College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China
  • College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China
  • College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China
  • College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China
  • College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China
  • State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China
  • College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, The People's Republic of China


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