Markery molekularne w badaniach rzepaku (Brassica napus L.). I. Przegląd stosowanych technik

• Autorzy: Matuszczak M.

Warianty tytułu

EN

Molecular markers for study of oilseed rape (Brassica napus L.) I. The review of marker techniques

• Języki publikacji: PL

Abstrakty

PL

Współczesna biologia molekularna dostarcza wciąż nowych metod pozwalających na opracowywanie markerów genetycznych, w tym przede wszystkim markerów molekularnych (markerów DNA). Znajdują one zastosowanie w różnych badaniach dotyczących rzepaku. Ich wykorzystanie w hodowli znacznie przyspiesza uzyskiwanie nowych odmian. Markery molekularne umożliwiają postęp w badaniach genomu. Istotne jest także znaczenie markerów molekularnych w badaniach pokrewieństwa oraz identyfikacji odmian. W pracy dokonano przeglądu technik poszukiwania markerów, które już znalazły lub mogą znaleźć zastosowanie w badaniach nad rzepakiem. Omówiono zarówno metody, które były stosowane na wczesnym etapie rozwoju tej dziedziny, jak i te, które stosowane są obecnie. Zasygnalizowano także prawdopodobne kierunki rozwoju nowych technologii dla pozyskiwania markerów w przyszłości.

EN

Modern molecular biology is the continuous source of new techniques that can be applied to obtain genetic markers, including, first of all, molecular (DNA) markers. Such markers are widely used for study of oilseed rape. Their application in breeding can speed up the formation of new varieties. Molecular markers can generate the progress in study of various genomes. There are many cases of their use for relationship analysis or variety identification. The review of marker techniques, both those already applied and as yet not applied for study on oilseed rape, is presented here. In this review past and present methods are described according to the sequence of their invention and use. The prospects of new technologies that may be used for marker development in the near future are also mentioned.

Słowa kluczowe

PL

rzepak; Brassica napus; markery genetyczne; markery molekularne; genotypowanie; hodowla roslin; metody wykrywania; markery izoenzymatyczne; markery RFLP; lancuchowa reakcja polimerazy; markery RAPD; markery AFLP-RGA; markery AFLP; markery DArT; mikrosatelity; markery ISSR; markery ACGM; markery SCAR; markery CAPS; markery SNP

EN

rape; Brassica napus; genetic marker; molecular marker; genotyping; plant breeding; detection method; isoenzyme marker; RFLP marker; polymerase chain reaction; RAPD marker; AFLP-RGA marker; AFLP marker; DArT marker; microsatellite; ISSR marker; ACGM marker; SCAR marker; CAPS marker; SNP marker

• Wydawca: -
• Czasopismo: Rośliny Oleiste - Oilseed Crops
• Rocznik: 2013
• Tom: 34
• Numer: 2
• Opis fizyczny: s.129-150,tab.,bibliogr.

Twórcy

autor

Matuszczak M.

• Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy, Oddział w Poznaniu

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