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2010 | 70 | 3 |
Tytuł artykułu

Activation of PKCα is required for migration of C6 glioma cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The PKC signaling pathway has been implicated in diverse cellular functions. Here, we sought to investigate the role of PKCα/β in C6 glioma cell migration. We found that both PKCα and PKCβ were expressed by C6 glioma cells, but only PKCα was markedly activated in serum-treated C6 cells. Gö6976, a PKC α/β specific inhibitor, was found to cause a dose-dependent reduction of PKCα activation and cell migration induced by serum in C6 cells. These results collectively indicated that the PKCα signaling pathway is necessary for glioma cell migration. Our findings may provide an insight into a better understanding to the malignant progression of gliomas.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
70
Numer
3
Opis fizyczny
p.239-245,fig.,ref.
Twórcy
autor
  • Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China
  • Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China
autor
  • Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China
autor
  • Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China
autor
  • Department of Clinical Laboratory Science, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China
autor
  • Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China
autor
  • Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China
  • Central laboratory, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China
Bibliografia
  • Ainsworth PD, Winstanley JH, Pearson JM, Bishop HM, Garrod DR (2004) Protein kinase C alpha expression in normal breast, ductal carcinoma in situ and invasive duc­tal carcinoma. Eur J Cancer 40: 2269-2273.
  • Amberger VR, Hensel T, Ogata N, Schwab ME (1998) Spreading and migration of human glioma and rat C6 cells on central nervous system myelin in vitro is corre­lated with tumor malignancy and involves a metallopro- teolytic activity. Cancer Res 58: 149-158.
  • Baines CP, Molkentin JD (2005) STRESS signaling path­ways that modulate cardiac myocyte apoptosis. J Mol Cell Cardiol 38: 47-62.
  • Bello L, Giussani C, Carrabba G, Pluderi M, Costa F, Bikfalvi A (2004) Angiogenesis and invasion in gliomas. Cancer Treat Res 117: 263-284.
  • Cameron, A Procyk KJ, Leitges M, Parker PJ (2008) PKC alpha protein but not kinase activity is critical for glioma cell proliferation and survival. Int J Cancer 123: 769-79.
  • Cannons JL, Yu LJ, Hill B, Mijares LA, Dombroski D, Nichols KE, Antonellis A, Koretzky GA, Gardner K, Schwartzberg PL (2004) SAP regulates T(H)2 differentia­tion and PKC-theta-mediated activation of NF-kappaB1. Immunity 21: 693-706.
  • Chida K, Hara T, Hirai T, Konishi C, Nakamura K, Nakao K, Aiba A, Katsuki M, Kuroki T (2003) Disruption of protein kinase Ceta results in impairment of wound heal­ing and enhancement of tumor formation in mouse skin carcinogenesis. Cancer Res 63: 2404-2408.
  • DeAngelis LM (2001) Brain tumors. N Engl J Med 344: 114-123.
  • Engers R, Mrzyk S, Springer E, Fabbro D, Weissgerber G, Gernharz CD, Gabbert HE (2000) Protein kinase C in human renal cell carcinomas: role in invasion and differ­ential isoenzyme expression. Br J Cancer 82: 1063­1069.
  • Griscelli F, Li H, Cheong C, Opolon P, Bennaceur-Griscelli A, Vassal G, Soria J, Soria C, Lu H, Perricaudet M, Yeh P (2000) Combined effects of radiotherapy and angiosta- tin gene therapy in glioma tumor model. Proc Natl Acad Sci U S A 97: 6698-6703.
  • Halet G (2004) PKC signaling at fertilization in mammalian eggs. Biochim Biophys Acta 1742: 185-189.
  • Kerfoot C, Huang W, Rotenberg SA (2004) Immunohistochemical analysis of advanced human breast carcinomas reveals downregulation of protein kinase C alpha. J Histochem Cytochem 52: 419-422.
  • Koivunen J, Aaltonen V, Koskela S, Lehenkari P, Laato M, Peltonen J (2004) Protein kinase C alpha/beta inhibitor Go6976 promotes formation of cell junctions and inhibits invasion of urinary bladder carcinoma cells. Cancer Res 64: 5693-5701.
  • Koivunen J, Aaltonen V, Peltonen J (2006) Protein kinase C (PKC) family in cancer progression. Cancer Lett 235: 1-10.
  • Koren R, Ben Meir D, Langzam L, Dekel Y, Konichezky M, Baniel J, Livne PM, Gal R, Sampson SR (2004) Expression of protein kinase C isoenzymes in benign hyperplasia and carcinoma of prostate. Oncol Rep 11: 321-326.
  • Langzam L, Koren R, Gal R, Kugel V, Paz A, Farkas A, Sampson SR (2001) Patterns of protein kinase C isoenzyme expression in transitional cell carcinoma of bladder. Relation to degree of malignancy. Am J Clin Pathol 116: 377-385.
  • Lu HZ and Hu JG (2009) Expression of bone morphoge- netic proteins-2/4 in neural stem cells and their lineages. Acta Neurobiol Exp 69: 441-447.
  • Makagiansar IT, Williams S, Dahlin-Huppe K, Fukushi J, Mustelin T, Stallcup WB (2004) Phosphorylation of NG2 proteoglycan by protein kinase C-alpha regulates polar­ized membrane distribution and cell motility. J Biol Chem 279:55262-55270.
  • Masur K, Lang K, Niggemann B, Zanker KS, Entschladen F (2001) High PKC alpha and low E-cadherin expression contribute to high migratory activity of colon carcinoma cells. Mol Biol Cell 12: 1973-1982.
  • Miguel BG, Fernández MI, Toboso I, Agudo-López A, Catalán E, Martínez AM (2008) Sphingosylphosphorylcholine induces mitochondria-mediated apoptosis in neuro 2a cells: Involvement of protein kinase C. Acta Neurobiol Exp (Wars) 68: 443-452.
  • Nakashima S (2002) Protein kinase C alpha (PKC alpha): regu­lation and biological function. J Biochem 132: 669-675.
  • Ng T, Shima D, Squire A, Bastiaens PI, Gschmeissner S, Humphries MJ, Parker PJ (1999) PKCalpha regulates beta1 integrin-dependent cell motility through association and control of integrin traffic. Embo J 18: 3909-3923.
  • Podar K, Tai YT, Lin BK, Narsimhan RP, Sattler M, Kijima T, Salgia R, Gupta D, Chauhan D, Anderson KC (2002) Vascular endothelial growth factor-induced migration of multiple myeloma cells is associated with beta 1 integrin- and phosphatidylinositol 3-kinase-dependent PKC alpha activation. J Biol Chem 277: 7875-7881.
  • Rabinovitz I, Tsomo L, Mercurio AM (2004) Protein kinase C-alpha phosphorylation of specific serines in the con­necting segment of the beta 4 integrin regulates the dynamics of type II hemidesmosomes. Mol Cell Biol 24: 4351-4360.
  • Tsai JH, Hsieh YS, Kuo SJ, Chen ST, Yu SY, Huang CY, Chang AC, Wang YW, Tsai MT, Liu JY (2000) Alteration in the expression of protein kinase C isoforms in human hepatocellular carcinoma. Cancer Lett 161: 171-175.
  • Varga A, Czifra G, Tallai B, Nemeth T, Kovacs I, Kovacs L, Biro T (2004) Tumor grade-dependent alterations in the protein kinase C isoform pattern in urinary bladder carci­nomas. Eur Urol 46: 462-465.
  • Yan P, Li Q, Kim GM, Xu J, Hsu CY, Xu XM (2001) Cellular localization of tumor necrosis factor-alpha fol­lowing acute spinal cord injury in adult rats. J Neurotrauma 18: 563-568.
  • Yokoyama G, Fujii T, Tayama K, Yamana H, Kuwano M, Shirouzu K (2005) PKCdelta and MAPK mediate G(1) arrest induced by PMA in SKBR-3 breast cancer cells. Biochem Biophys Res Commun 327: 720-726.
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Bibliografia
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