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2019 | 78 | 2 |
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Combination of vitamin E and L-carnitine is superior in protection against isoproterenol-induced cardiac injury: histopathological evidence

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Background: L-carnitine and vitamin E have antioxidant properties. This study aimed to assess the effectiveness of L-carnitine, vitamin E and their combination in protection against isoproterenol (ISO)-induced biochemical and histopathological changes in rat heart. Materials and methods: Fifty male Wistar rats assigned to five groups; control, ISO-treated group (100 mg/kg), ISO + vitamin E-treated group (100 IU/kg), ISO + L-carnitine (100 mg/kg) and ISO + vitamin E + L-carnitine treated group. At the end of the experiment, serum cardiac enzyme as well as the cardiac level malondialdehyde (MDA), antioxidant enzymes and inflammatory cytokines interleukin-6, tumour necrosis factor alpha (TNF-α) were assessed. Histopathological changes in the left ventricle wall were assessed using the light and electron microscopy. Results: Treating rats with vitamin E and L-carnitine could alleviate ISO-induced changes as it significantly reduced the serum level cardiac enzymes, MDA and IL-6, TNF-α and improved the antioxidants enzymes (SOD, GSPxase and GSRase). Histopathologically, they improved cardiac fibres atrophy, haemorrhages between cardiac fibres, lost striations, and disturbed sarcomere structure. The combined effect of vitamin E and L-carnitine was more superior compared to the other groups. Conclusions: Combined administration of vitamin E, L-carnitine ameliorated the biochemical and histopathological cardiac injury induced by ISO. The effect seemed to be mediated through the antioxidant and anti-inflammatory effect of vitamin E, L-carnitine. Administration of these two elements is recommended for patient at risk for myocardial infarction. (Folia Morphol 2019; 78, 2: 274–282)
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  • Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
  • 1. Ahmed MM, Ibrahim ZS, Alkafafy M, et al. L-carnitine protects against testicular dysfunction caused by gamma irradiation in mice. Acta Histochem. 2014; 116(6): 1046–1055, doi: 10.1016/j.acthis.2014.04.010, indexed in Pubmed: 24925768.
  • 2. Alshiek JA, Dayan L, Asleh R, et al. Anti-oxidative treatment with vitamin E improves peripheral vascular function in patients with diabetes mellitus and Haptoglobin 2-2 genotype: A double-blinded cross-over study. Diabetes Res Clin Pract. 2017; 131: 200–207, doi: 10.1016/j.diabres.2017.06.026, indexed in Pubmed: 28759833.
  • 3. Bancroft JD, Gamble M. Theory and practice of histological techniques. 6th ed. Churchill Livingstone, Elsevier, China. 2008.
  • 4. Boaz M, Smetana S, Weinstein T, et al. Secondary prevention with antioxidants of cardiovascular disease in endstage renal disease (SPACE): randomised placebo-controlled trial. Lancet. 2000; 356(9237): 1213–1218, indexed in Pubmed: 11072938.
  • 5. De Bono DP, Boon NA. Diseases of the cardiovascular system. In: Edwards CRW, Bouchier IAS, eds. Davidson’s Principles of Practice and Medicine. Hong Kong: Churchill Livingstone. 1992: 249–340.
  • 6. Deore AB, Vinayak D. Sapakal and Naikwade NS: Antioxidant and hepatoprotective activity of Garcinia indica Linn fruit rind. J Comprehensive Pharmacy. 6.08.
  • 7. Dhivya V, Priya LB, Chirayil HT, et al. Piperine modulates isoproterenol induced myocardial ischemia through antioxidant and anti-dyslipidemic effect in male Wistar rats. Biomed Pharmacother. 2017; 87: 705–713, doi: 10.1016/j.biopha.2017.01.002, indexed in Pubmed: 28088738.
  • 8. Gayathri K, Jayachandran KS, Vasanthi HR, et al. Cardioprotective effect of lemon grass as evidenced by biochemical and histopathological changes in experimentally induced cardiotoxicity. Hum Exp Toxicol. 2011; 30(8): 1073–1082, doi: 10.1177/0960327110386391, indexed in Pubmed: 20952451.
  • 9. Goyal S, Siddiqui MK, Siddiqui KM, et al. Cardioprotective effect of ‘Khamira Abresham Hakim Arshad Wala’ a unani formulation in isoproterenol-induced myocardial necrosis in rats. Exp Toxicol Pathol. 2010; 62(1): 61–74, doi: 10.1016/j.etp.2009.02.115, indexed in Pubmed: 19285846.
  • 10. Goyal SN, Arora S, Sharma AK, et al. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine. 2010; 17(3-4): 227–232, doi: 10.1016/j.phymed.2009.08.009, indexed in Pubmed: 19747807.
  • 11. Goyal SN, Sharma C, Mahajan UB, et al. Protective effects of cardamom in isoproterenol-induced myocardial infarction in rats. Int J Mol Sci. 2015; 16(11): 27457–27469, doi: 10.3390/ijms161126040, indexed in Pubmed: 26593900.
  • 12. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22): 7130–7139, indexed in Pubmed: 4436300.
  • 13. Hu XX, Fu Li, Li Y, et al. The cardioprotective effect of vitamin e (alpha-tocopherol) is strongly related to age and gender in mice. PLoS One. 2015; 10(9): e0137405, doi: 10.1371/journal.pone.0137405, indexed in Pubmed: 26331272.
  • 14. Lango R, Smolenski RT, Narkiewicz M, et al. Influence of L-carnitine and its derivatives on myocardial metabolism and function in ischemic heart disease and during cardiopulmonary bypass. Cardiovasc Res. 2001; 51(1): 21–29, indexed in Pubmed: 11399244.
  • 15. Lee BJ, Lin JS, Lin YC, et al. Effects of L-carnitine supplementation on lipid profiles in patients with coronary artery disease. Lipids Health Dis. 2016; 15: 107, doi: 10.1186/s12944-016-0277-5, indexed in Pubmed: 27317162.
  • 16. Lee IM, Cook N, Gaziano J, et al. Vitamin E in the primary prevention of cardiovascular disease and cancer. The Women’s Health Study: a randomized controlled trial. JAMA. 2005; 294(1): 56–65, doi: 10.1001/jama.294.1.56.
  • 17. Li Li, Zhu Di, Sun X. [Cellular uptake of TPS-L-carnitine synthesised as transporter-based renal targeting prodrug]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2012; 43(6): 936–940, indexed in Pubmed: 23387232.
  • 18. Liepinsh E, Makrecka-Kuka M, Kuka J, et al. Inhibition of L-carnitine biosynthesis and transport by methyl-g-butyrobetaine decreases fatty acid oxidation and protects against myocardial infarction. Br J Pharmacol. 2015; 172(5): 1319–1332, doi: 10.1111/bph.13004, indexed in Pubmed: 25363063.
  • 19. Panteghini M. Role and importance of biochemical markers in clinical cardiology. Eur Heart J. 2004; 25(14): 1187–1196, doi: 10.1016/j.ehj.2004.04.026.
  • 20. Milman U, Blum S, Shapira C, et al. Vitamin E supplementation reduces cardiovascular events in a subgroup of middleaged individuals with both type 2 diabetes mellitus and the haptoglobin 2-2 genotype: a prospective double-blinded clinical trial. Arterioscler Thromb Vasc Biol. 2008; 28(2): 341–347, doi: 10.1161/ATVBAHA.107.153965, indexed in Pubmed: 18032779.
  • 21. Moran MS. Levels of glutathione, glutathione reductase and glutathione-S-transferase activity in rat lung and liver. Biochim et Biophys. 1979; 582: 67–78.
  • 22. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1): 158–169, indexed in Pubmed: 6066618.
  • 23. Panda S, Kar A. Combined effects of vincristine and quercetin in reducing isoproterenol-induced cardiac necrosis in rats. Cardiovasc Toxicol. 2015; 15(4): 291–299, doi: 10.1007/s12012-014-9291-x, indexed in Pubmed: 25537132.
  • 24. Panda V, Kamble S, Desai Y, et al. Antioxidant and cardioprotective effects of Garcinia indica (kokoberry), an Indian super fruit in isoproterenol induced myocardial necrosis in rats. J Berry Res. 2014; 4(3): 159–174, doi: 10.3233/jbr-140078.
  • 25. Salama A, Kasem S, Tousson E, et al. L-carnitine and vitamin E alleviate reproductive toxicity caused by triton WR 1339 in male albino rats. Toxicol Ind Health. 2015; 31(5): 467–474.
  • 26. Schönekess B, Lopaschuk G. The effects of carnitine on myocardial carbohydrate metabolism. The Carnitine System. A New Therapeutical Approach to Cardiovascular Diseases, edited by De Jong JW, Ferrari R, Dordrecht, Kluwer Academic Publishers. 1995: 39–52, doi: 10.1007/978-94-011-0275-9_4.
  • 27. Shang R, Sun Z, Li H. Effective dosing of L-carnitine in the secondary prevention of cardiovascular disease: a systematic review and meta-analysis. BMC Cardiovasc Disord. 2014; 14: 88, doi: 10.1186/1471-2261-14-88, indexed in Pubmed: 25044037.
  • 28. Shukla SK, Sharma SB, Singh UR, et al. Eugenia jambolana pretreatment prevents isoproterenol-induced myocardial damage in rats: evidence from biochemical, molecular, and histopathological studies. J Med Food. 2014; 17(2): 244–253, doi: 10.1089/jmf.2013.2795, indexed in Pubmed: 24325453.
  • 29. Tappia PS, Hata T, Hozaima L, et al. Role of oxidative stress in catecholamine-induced changes in cardiac sarcolemmal Ca2+ transport. Arch Biochem Biophys. 2001; 387(1): 85–92, doi: 10.1006/abbi.2000.2234, indexed in Pubmed: 11368187.
  • 30. Thygesen K, Mair J, Katus H, et al. Acute Cardiac Care. Recomendations for the use of cardiac troponin measurement in acute cardiac care. Eur Heart J. 2010; 31(18): 2197–2204, doi: 10.1093/eurheartj/ehq251, indexed in Pubmed: 20685679.
  • 31. Tousson E, Hafez E, Zaki S, et al. The cardioprotective effects of L-carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin. Environ Sci Pollut Res Int. 2016; 23(20): 20600–20608, doi: 10.1007/s11356-016-7220-1, indexed in Pubmed: 27464663.
  • 32. Whellan D. Heart failure disease management. Implementation and outcomes. Cardiol Rev. 2005; 13(5): 231–239, doi: 10.1097/01.crd.0000135765.60824.2f.
  • 33. Wong GK, Pehora C, Crawford MW. L-carnitine reduces susceptibility to bupivacaine-induced cardiotoxicity: an experimental study in rats. Can J Anaesth. 2017; 64(3): 270–279, doi: 10.1007/s12630-016-0797-5, indexed in Pubmed: 28032304.
  • 34. Yüncü M, Bükücü N, Bayat N, et al. The effect of vitamin E and L-carnitine against methotrexate-induced injury in rat testis. Turk J Med Sci. 2015; 45(3): 517–525, indexed in Pubmed: 26281314.
  • 35. Zambrano S, Blanca AJ, Ruiz-Armenta MV, et al. L-carnitine protects against arterial hypertension-related cardiac fibrosis through modulation of PPAR-g expression. Biochem Pharmacol. 2013; 85(7): 937–944, doi: 10.1016/j.bcp.2012.12.021, indexed in Pubmed: 23295156.
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