PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 32 | 6 |
Tytuł artykułu

Improvement of biomass and juvenile hormone III (JH III) production from Cyperus aromaticus cell suspension culture via in situ membrane filtration technology

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The incorporation of membrane filtration technology in plant cell suspension culture was to facilitate in situ medium exchange as well as to eliminate possible introduction of contaminant in the process. The study had been carried out in Cyperus aromaticus cell suspension culture, which is known to produce a bioinsecticide known as juvenile hormone III (JH III). The effect of different volume and flow rate of replenishment toward cell biomass and JH III production were studied. The experimental finding indicates that the flow rate of medium replenishment ranging from 1 to 6 ml min⁻¹ did not affect the cellular structure and morphology the cells. Medium replenishment of 75 v/v% through the membrane system leads to higher cell biomass and metabolite production which correlates to a value as high as 64 and 112%, respectively. The reduction in doubling time, extended exponential growth and increment in specific growth rate were also demonstrated. A 50 v/v% medium replenishment with membrane filtration system was identified to be an economical and feasible application for the production of JH III metabolite at a large scale. The results obtained showed the potential of in situ membrane filtration system for the enhancement of cell biomass and metabolite yield in plant cell culture.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
32
Numer
6
Opis fizyczny
p.1153-1159,fig.,ref.
Twórcy
autor
  • School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia
autor
  • School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia
autor
  • Plant Tissue and Cell Culture Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
Bibliografia
  • Bede JC, Goodman WG, Tobe SS (1999) Developmental distribution of insect juvenile hormone III in the sedge, Cyperus iria L. Phytochemistry 52:1269–1274
  • Chan CW (2005) Micropropagation and callus induction of Cyperus aromaticus for the production of juvenile hormone III. MSc Thesis, Universiti Sains, Malaysia, Penang
  • Chan CW, Chan LK, Boey PL (2004) Detection of insect juvenile hormone III and its precursors from in vitro plantlets of Cyperus aromaticus. J Plant Biol 47(3):187–193
  • Choi HJ, Tao BY, Okos MR (1995) Enhancement of secondary metabolite production by immobilized Gossypium arboretum cells. Biotechnol Prog 11:306–311
  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 3:350–356
  • Dunlop EH, Namdev PK (1994) Effect of fluid shear forces on plant cell suspensions. Chem Eng Sci 49(14):2263–2276
  • Edahiro JC, Seki M (2006) Phenylpropanoid metabolite supports cell aggregate formation in strawberry cell suspension culture. J Biosci Bioeng 102:8–13
  • Eddie J, David RM, James ML (2002) Increased production and recovery of secreted foreign proteins from plant cell culture using affinity chromatography bioreactor. Biochem Eng J 12:205–213
  • Gaudin V, Lunnes PA, Fobert PR, Towers M, Riou-Khamlichi C, Murray JAH, Coen E, Doonan JH (2000) The expression of D-cyclin genes defines distinct developmental zones in snapdragon apical meristems and is locally regulated by the Cyctoidea gene. Plant Physiol 122:1137–1148
  • Gillet F, Roisin C, Fliniaux MA, Jacquin-Dubreuil A, Barbotin JN, Nava-Saucedo JE (2000) Immobilization of Nicotiana tabacum plant cell suspensions within calcium alginate gel beads for the production of enhanced amounts of scopolin. Enzyme Microb Technol 26:229–234
  • Gould AR, Everett NP, Wang TL, Street HE (1981) Studies on the control of the cell cycle in cultured plant cells I. Effect of nutrient limitation and nutrient starvation. Protoplasma 106:1–13
  • Kamoshita Y, Ohashi R, Suzuki T (1998a) Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis. J Ferment Bioeng 85:422–427
  • Kamoshita Y, Ohashi R, Suzuki T (1998b) A dense cell culture system for aerobic microorganisms using shaken ceramic membrane flask with surface aeration. J Ferment Bioeng 85:218–222
  • Kim JH, Yun JH, Hwang YS, Byun SY, Kim DI (1995) Production of taxol and related taxanes in Taxus brevifolia cell culture: effect of sugar. Biotechnol Lett 17:101–106
  • Lim PS, Chan LK (2005) Detection of juvenile hormone III (JHIII) in cell suspension cultures of Cyperus aromaticus. In: Proceedings of 20th scientific meeting of the Malaysian society of pharmacology and physiology nutraceuticals: the scientific basis, Penang, Malaysia
  • Meijer JJ, Hoopen HJG, van Gameren YM, Luyben AM, Libbenga KR (1994) Effects of hydrodynamic stress on the growth of plant cells in batch and continuous culture. Enzyme Microbial Technol 16(6):467–477
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Ohashi R, Kamoshita Y, Kismimota M, Suzuki T (1998) Continuous production and separation of ethanol without effluence of wastewater using a distiller integrated SCM-reactor system. J Ferment Bioeng 86(2):220–225
  • Ohashi R, Yamamoto T, Suzuki T (1999) Continuous production of lactic acid from molasses by perfusion culture of Lactococcus lactis using a stirred ceramic membrane reactor. J Biosci Bioeng 87(5):647–654
  • Riou-Khamlichi C, Menges M, Sandra Healy JM, Murray JAH (2000) Sugar control of the plant cell cycle: differential regulation of Arabidopsis D-type cyclin gene expression. Mol Cell Biol 20(13):4513–4521
  • Suzuki T (1996) A dense cell culture system for microorganisms using a stirred ceramic membrane reactor incorporating asymmetric porous ceramic filters. J Ferment Bioeng 82:264–271
  • Toong YC, Schooley DA, Baker FC (1988) Isolation of insect juvenile hormone III from a plant. Nature 333:170–171
  • Wang HQ, Yu JT, Zhong JJ (2000) Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy. Process Biochem 35:479–483
  • Wang C, Wu J, Mei X (2001) Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding. Biotechnol Prog 17:89–94
  • Zhang YH, Zhong JJ (1997) Hyperproduction of ginseng saponin and polysaccharide by high density cultivation of Panax notoginseng cells. Enzyme Microbial Technol 21:59–63
  • Zhong JJ, Yoshida T (1995) High density cultivation of Perilla frutescens cell suspensions for anthocyanin production: Effect of sucrose concentration and inoculum’s size. Enzyme Microbial Technol 17:1073–1079
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-0d9fe8c3-eaaa-43b4-b117-a41707faa10c
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.