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2010 | 64 |
Tytuł artykułu

Micropropagation of Calycanthus fertilis

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on methodpropagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg·dm–3) and TDZ (from 0.1 to 0.5 mg·dm–3). BAP turnedout to significantly increase initiation frequency whereas TDZ inhibitedthe formation of adventitious shoots andcausedexplant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg·dm–3 BAP. Primary explants which initiatedgrowth were transferredon the proliferation media containing WPM macroand microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg·dm–3), KIN (1.0 to 5.0 mg·dm–3) or TDZ (from 0.1 to 0.5 mg·dm–3). Calycanthus multiplication in vitro shouldbe conductedon WPM media with 1.0 mg·dm–3 BAP. Proliferatedshoots were placedon the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg·dm–3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg·dm–3 IBA. To sum up, it shouldbe statedthat an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.
Wydawca
-
Czasopismo
Rocznik
Tom
64
Opis fizyczny
p.31-35,fig.,ref.
Twórcy
autor
  • Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland,
autor
Bibliografia
  • Babu K.N., Sajina A., Minoo D., John C.Z., Mini P.M., Tushar K.V., Rema J., Ravindran P.N. 2003. Micropropagation of camphor tree (Cinnamomum camphora). Plant Cell, Tissue andOrgan Culture 74: 179–183.
  • Barcelo-Mu oz A., Encina C.L., Simon-Perez E., Pliego-Alfaro F. 1999. Micropropagation of adult avocado. Plant Cell, Tissue and Organ Culture 58: 11–17.
  • Chant S.R. 1978. Calycanthaceae. In: HeywoodV. H (ed.) Flowering Plants of the World. Elsevier, Oxford, pp. 35–36.
  • Goremykin V., Hirsch-Ernst K.I., Wolfl S., Hellwig F.H. 2003. The chloroplast genome of the ‘‘basal’’ angiosperm Calycanthus fertilis – structural and phylogenetic analyses. Plant Systematics and Evolution 242: 119–135.
  • Huang L.-Ch., Huang B-L., Murashige T. 1998. A micropropagation protocol for Cinnamomum camphora. In Vitro Cellular & Developmental Biology – Plant 34:141–146.
  • Krüssmann G. 1985. Manual of CultivatedConifers, 2nd ed. Timber Press, Portland, Oregon.
  • LloydGB., McCown B. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings – International Plant Propagators’ Society 30: 421–427.
  • Murashige T., Skoog F. 1962. A revisedmed ium for rapidgrowth andbioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–479.
  • Nowakowska M., Baran J. 2007. Uszkodzenia mrozowe drzew i krzewów rosnących w Ogrodzie Dendrologicznym w Glinnej podczas zimy 2005/2006. Roczniki Dendrologiczne 55: 129–140.
  • Renner S.S. 1999. Circumscription andphylogeny of the Laurales: evidence from molecular and morphological data. American Journal of Botany 86: 1301–1315.
  • Seneta W. 1996. Drzewa i krzewy liściaste. Tom II Callicarpa-Cytisus. Wydawnictwo Naukowe PWN: 16–21.
  • Shao G., Cai R., Wang L., Liang G.-L. 2006. Study on Tissue Culture of Calycanthus chinensis. Journal of Zhejiang Forestry Science andTechnology 2006: 05. http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZJLK200605008.htm.
  • Shi X.P., Dai XG., Liu G.F., Zhang J.W., Ning G.G., Bao M.Z 2010. Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.). In Vitro Cellular & Developmental Biology – Plant 46: 117–125.
  • Witjaksono, Schaffer B.A., Colls A.M., Litz R.E., Moon P.A. 1999. Avocado shoot culture, plantlet development and net CO2 assimilation in an ambient andCO 2 enhancedenvironment. In Vitro Cellular & Developmental Biology – Plant 35: 238–244.
  • Witjaksono. 1991. Tissue culture medium for ‘Pinkerton’ avocado (Persea Americana Mill.). In: Proceedings of the Seminar and National Congress on Biology X, Bogor: Indonesian Biological Society and IUC Life Science, IPB; pp. 411–417.
Typ dokumentu
Bibliografia
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