Female mouse model of diabetes mellitus induced by streptozotocin and high-carbohydrate high-fat diet

• Autorzy: Guo R. , Dong J. , Wang D.Q. , Gu Y.F.
• Języki publikacji: EN

Abstrakty

EN

With the improvement of people’s living standards and rapid economic development, the incidence of diabetes mellitus (DM) is increasing in most parts of the world. DM presents an important potential threat to human health. In the present study, a model of diabetes in female mice was established, and fasting blood glucose was detected at week 4, after which the biochemical profiles were evaluated by histopathological analysis. The success rate of modeling in the normal control (NC) group and the low/ middle/high-dose streptozotocin (STZ) group were 0, 0, 25% and 60%, respectively. In the middle-dose and high-dose STZ groups, the liver index was increased significantly compared with the NC group (p<0.05). The blood biochemical indicators of total cholesterol and low density lipoprotein cholesterol in three STZ injection groups were as follows: alanine aminotransferase and aspartate transaminase in middle- and high-dose STZ groups, high-density lipoprotein cholesterol and serum creatinine in the high-dose STZ group, and blood urea nitrogen in the middle-dose STZ group were significantly increased (p<0.05). The level of total triglycerides was lower, obviously, in the high-dose STZ group than in the NC group (p<0.05). The mice showed marked steatosis, green-dyed fiber tissue coloring in varying degrees, and the contour of the hepatic lobules basically disappeared in STZ injection groups. The results suggest that to establish a diabetes model for female ICR mice, the optimum dose of STZ is 100 mg/kg.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 4
• Opis fizyczny: p.547-555,fig.,ref.

Twórcy

autor

Guo R.

• State Key Laboratory for Diagnosis and Treatment of Infectious Disease, National Clinical Research Center for Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
• inan Microecological Biomedicine Shandong Laboratory, No. 3716 Qingdao Road, Huaiyin District, Jinan City, Shandong Province, Solutia City Light West Building, 21F, Shandong Laboratory of Microecological Biomedicine, Jinan 250117, China

autor

Dong J.

• Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

autor

Wang D.Q.

• nstitute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

autor

Gu Y.F.

• State Key Laboratory for Diagnosis and Treatment of Infectious Disease, National Clinical Research Center for Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
• inan Microecological Biomedicine Shandong Laboratory, No. 3716 Qingdao Road, Huaiyin District, Jinan City, Shandong Province, Solutia City Light West Building, 21F, Shandong Laboratory of Microecological Biomedicine, Jinan 250117, China

Identyfikatory

DOI

10.24425/pjvs.2022.142042