Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants

• Autorzy: Wojtal I. , Piontek P. , Grzela R. , Jarmolowski A. , Zagorski W. , Chroboczek J.
• Języki publikacji: EN

Abstrakty

EN

 Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.

Słowa kluczowe

EN

potyvirus; VPg protein; transgenic plant; Arabidopsis thaliana; potato virus Y; life cycle; VPg gene; pathogen-derived resistance

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2011
• Tom: 58
• Numer: 3
• Opis fizyczny: p.349-353,fig.,ref.

Twórcy

autor

Wojtal I.

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Piontek P.

autor

Grzela R.

autor

Jarmolowski A.

autor

Zagorski W.

autor

Chroboczek J.

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