Starch and sugar accumulation in Sulla carnosa leaves upan Mg 2plus starvation

• Autorzy: Farhat N. , Rabhi M. , Krol M. , Barhoumi Z. , Ivanov A.G. , McCarthy A. , Abdelly C. , Smaoui A. , Huner N.P.A.
• Języki publikacji: EN

Abstrakty

EN

In the present work, magnesium deficiency effects were studied in Sulla carnosa plants grown in nutrient solution containing 1.50, 0.05, 0.01, and 0.00 mM Mg²⁺. After 5 weeks of treatment, fully expanded leaves were harvested to study their morphological and ultrastructural changes, as well as their carbohydrate, pigment, and Mg²⁺ concentrations. In control plants, leaves were well developed with remarkable green color. Down to 0.05 mM Mg²⁺, no chlorosis symptom was recorded, but below this concentration, mature leaves showed an appearance of interveinal chlorosis that was much more pronounced at 0.00 mM Mg²⁺ with the development of necrotic spots. Optima of chlorophyll a, chlorophyll b, and carotenoid concentrations were observed at 0.05 and 1.50 mM Mg²⁺; leaf magnesium concentration was severely reduced at 0.05 mM Mg²⁺. A significant decrease in pigment concentrations was noticed at 0.01 mM Mg²⁺, but the lowest values were recorded at 0.00 mM Mg²⁺. Enzymatic assays showed an increase in the accumulation of soluble sugars and starch with decreasing Mg²⁺ concentration. These results were in accordance with those of ultrastructural studies that revealed a marked alteration of chloroplasts in leaves of deficient plants. These chloroplasts were round and bigger as a result of a massive accumulation of oversized starch grains with disrupted thylakoids. As a whole, 1.50, 0.05, and 0.01 mM Mg²⁺ were found optimal, suboptimal, and deficient concentrations, respectively, the latter showing no significant difference with absolute Mg²⁺ absence (0.00 mM Mg²⁺).

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 08
• Opis fizyczny: p.2157-2165,fig.,ref.

Twórcy

autor

Farhat N.

• Laboratory of Extremophile Plants, Biotechnoogy Centre of Borj Cedria, P.O. Box 901, 2050 Hammam-Lif, Tunisia

autor

Rabhi M.

• Laboratory of Extremophile Plants, Biotechnoogy Centre of Borj Cedria, P.O. Box 901, 2050 Hammam-Lif, Tunisia

autor

Krol M.

• Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada

autor

Barhoumi Z.

• Laboratory of Extremophile Plants, Biotechnoogy Centre of Borj Cedria, P.O. Box 901, 2050 Hammam-Lif, Tunisia

autor

Ivanov A.G.

• Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada

autor

McCarthy A.

• Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada

autor

Abdelly C.

• Laboratory of Extremophile Plants, Biotechnoogy Centre of Borj Cedria, P.O. Box 901, 2050 Hammam-Lif, Tunisia

autor

Smaoui A.

• Laboratory of Extremophile Plants, Biotechnoogy Centre of Borj Cedria, P.O. Box 901, 2050 Hammam-Lif, Tunisia

autor

Huner N.P.A.

• Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada

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Identyfikatory

DOI

10.1007/s11738-014-1592-y