PCR - RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains

• Autorzy: Klesiewicz K. , Nowak P. , Karczewska E. , Skiba I. , Wojtas-Bonior I. , Sito E. , Budak A.
• Języki publikacji: EN

Abstrakty

EN

Background: The occurrence of clarithromycin resistance among Helicobacter pylori strains is a major cause of the treatment failure. Resistance to this drug is conferred by point mutations in 23S rRNA gene and the most prevalent mutations are A2143G and A2142G. The aim of the study was to evaluate the occurrence of A2143G and A2142G mutations in a group of H. pylori strains resistant to clarithromycin. Materials and Methods: The study included 21 clarithromycin-resistant H. pylori strains collected between 2006 and 2009 in southern Poland. Resistance to clarithromycin was quantitatively tested with the E-test to determine the minimal inhibitory concentration (MIC value). The point mutations of H. pylori isolates were detected by PCR followed by RFLP analysis. Results: The MIC values for clarithromycin for the analyzed strains ranged from 1.5 mg/L to 64 mg/L. Nine H. pylori strains exhibited A2143G mutation and A2142G mutation was found in 9 isolates as well. The results of RFLP analysis of 3 clarithromycin-resistant strains were negative for both mutations. The average MIC values for A2143G and A2142G mutants were 6 and 30 mg/L, respectively. Conclusions: Frequencies of A2143G and A2142G mutations were the same in all isolates tested. Strains with A2143G mutation exhibited lower MIC values than A2142G mutants. Application of PCR-RFLP method for detection of clarithromycin resistance allows for better and more efficient management of H. pylori infections.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2014
• Tom: 61
• Numer: 2
• Opis fizyczny: p.311-315,fig.,ref.

Twórcy

autor

Klesiewicz K.

• Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Nowak P.

• Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Karczewska E.

• Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Skiba I.

• Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Wojtas-Bonior I.

• Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Krakow, Poland

autor

Sito E.

• Falck Medycyna Outpatient Clinic of Gastroenterology, Krakow, Poland

autor

Budak A.

• Department of Pharmaceutical Microbiology, Jagiellonian University Medical College, Krakow, Poland

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