Influence of addition of cobalamin to the extender on the post-thaw motility, viability, and DNA integrity of bovine ejaculate

• Autorzy: Mizera A. , Kuczaj M. , Szul A. , Jedraszczyk J.
• Języki publikacji: EN

Abstrakty

EN

The present study was undertaken to investigate the effect of various concentrations of cobalamin (vitamin B₁₂) in Bioexcell® extender on the spermatozoa quality of Simmental bulls. The semen was collected from 12 bulls by means of an artificial vagina. Vitamin B₁₂ at concentrations of 1.50, 2.50, 3.50, and 5.00 mg/mL was added to bovine Bioexcell® extender. The semen aliquots were cooled and preserved at 4°C. Their quality was evaluated during pre-freezing, and then the cooled semen samples were packaged into 0.25 ml straws. The straws were frozen in the vapor of liquid nitrogen, and stored at -196ºC in a container. The straws were thawed one day later, and the characteristics of spermatozoa were examined. The results showed that the effect of vitamin B₁₂ on the viability, DNA fragmentation and motility of spermatozoa was significant under both pre- and post-freezing conditions (p <0.05), and revealed that supplementation of the extender with B₁₂ improved the post-thaw spermatozoa quality in bulls.

Słowa kluczowe

EN

bull; Simmental breed; spermatozoon quality; vitamin B12; addition; extender; semen freezing; motility; viability; DNA integrity; bovine ejaculate; ejaculate; DNA fragmentation

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2019
• Tom: 75
• Numer: 03
• Opis fizyczny: p.164-168,fig.,ref.

Twórcy

autor

Mizera A.

• Institute of Animal Breeding, Faculty of Biology and Animal Science, Wroclaw Univeristy of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland

autor

Kuczaj M.

• Institute of Animal Breeding, Faculty of Biology and Animal Science, Wroclaw Univeristy of Environmental and Life Sciences, Chelmonskiego 38C, 51-630 Wroclaw, Poland

autor

Szul A.

• Malopolskie Centrum Biotechniki Sp. z o.o., Krasne 32, 36-007 Krasne, Poland

autor

Jedraszczyk J.

• Malopolskie Centrum Biotechniki Sp. z o.o., Krasne 32, 36-007 Krasne, Poland

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Identyfikatory

DOI

10.21521/mw.6183