Comparative growth kinetic study of Newcastle disease virus, infectious bursal disease virus and avian influenza virus in chicken embryo fibroblast and DF-1 cell lines

• Autorzy: Anam S. , Rahman S.U. , Ali S. , Saeed M. , Goyal S.M.
• Języki publikacji: EN

Abstrakty

EN

Viral diseases have caused devastating effect on poultry industry leading to significant losses in economy of world. In the presented study, the ability of Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and avian influenza virus (AIV) to grow in two cell lines was evaluated. Both chicken embryo fibroblast (CEF) and DF-1 cells were used and cytopathic effects (CPE) produced by these viruses were observed. The titer of virus in terms of TCID50 was determined after 24h up to four days for each virus. The same type of CPE was observed for all viruses used in the study in both DF-1 and CEF cells. IBDV showed CPE causing rounding of cells while NDV caused formation of multicellular large nuclei, cell fusion and rounding of cells. Gi-ant cells with inclusions and aggregation of cells with intact monolayer was observed for AIV. In growth kinetic study, higher titer of IBDV and NDV was observed in CEF cells than DF-1 cells while for AIV, DF-1 cells showed higher titer than CEF cells. These results would be useful for furthers comparative studies on growth of different cell lines of various viruses to find a suitability for vaccine production.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2021
• Tom: 24
• Numer: 2
• Opis fizyczny: p.287-292,fig.,ref.

Twórcy

autor

Anam S.

• Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan

autor

Rahman S.U.

• Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan

autor

Ali S.

• Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan

autor

Saeed M.

• National Institute of Food Science and Technology, University of agriculture Faisalabad, Pakistan

autor

Goyal S.M.

• College of Veterinary Population Medicine, 1333 Gortner Avenue, University of Minnesota, USA

Identyfikatory

DOI

10.24425/pjvs.2021.137664