Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 37 | 01 |
Tytuł artykułu

Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce

Warianty tytułu
Języki publikacji
Somatic embryo quality is still a problem for many researchers. To improve the efficiency of germination, special procedures are used, such as partial drying of somatic embryos at high relative humidity or desiccation in the presence of supersaturated solutions of salt. In this work, cotyledonary somatic embryos of Norway spruce (Picea abies) and Serbian spruce (P. omorika) were placed on culture media (ME or BM-5) to germinate. We found that after 4 weeks of incubation on these media, hypocotyl and radicle growth of control (non-dried) somatic embryos of both species was not adequate to yield seedlings able to acclimatize to greenhouse conditions. Therefore, somatic embryos were partly dried at relative humidity of 97 % or desiccated at relative humidity of 79 %, for 2 or 3 weeks, and then placed on the Margara (ME) medium. Partial drying of somatic embryos at the higher relative humidity (97 %) enabled an improvement of radicle growth of germinating somatic embryos in both species. The highest conversion rate (45 %) was obtained for embryos of Norway spruce maintained for 2 weeks at relative humidity of 97 %. This treatment contributed to the improvement of germination and conversion efficiency of somatic embryos of Norway spruce, regardless of the drying period. Improved radicle growth facilitated development of better quality seedlings of this spruce species. In Serbian spruce, we did not obtain seedlings of sufficient quality, due to poor hypocotyl growth. Desiccation at humidity of 79 % for 3 weeks proved to be lethal to somatic embryos of both species.
Słowa kluczowe
Opis fizyczny
Article: 1735 [9 p.], fig.,ref.
  • Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kornik, Poland
  • University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland
  • Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kornik, Poland
  • Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kornik, Poland
  • Angelovici R, Galili G, Fernie AR, Fait A (2010) Seed desiccation: a bridge between maturation and germination. Trends Plant Sci 15(4):211–218
  • Attree SM, Moore D, Sawhney VK, Fowke LC (1991) Enhanced maturation and desiccation tolerance of white spruce (Picea glauca [Moench] Voss) somatic embryos: effect of a nonplasmolysing water stress and abscisic acid. Ann Bot 68:519–525
  • Attree SM, Pomeroy MK, Fowke LC (1994) Production of vigorous, desiccation tolerant white spruce (Picea glauca [Moench] Voss) synthetic seeds in bioreactor. Plant Cell Rep 13:601–606
  • Attree SM, Pomeroy MK, Fowke LC (1995) Development of white spruce (Picea glauca (Moench) Voss) somatic embryos during culture with abscisic acid and osmoticum and their tolerance to drying and frozen storage. J Exp Bot 46:433–439
  • Becwar MR (1993) Conifer somatic embryogenesis and clonal forestry. In: Ahuja MR, Libby WJ (eds) Clonal forestry I genetics and biotechnology. Springer, Berlin, pp 201–223
  • Bercetche J (1989) Optimisation des conditions d’obtention de plantules a partier de cals embryogenes chez Picea abies. Annal Afocel, pp 97–115
  • Bomal C, Tremblay FM (1999) Effect of desiccation to low moisture content on germination, synchronisation of root emergence, and plantlet regeneration of black spruce somatic embryos. Plant Cell Tiss Org Cult 56:193–200
  • Bozhkov PV, von Arnold S (1998) Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiol Plant 104:211–224
  • David A, Laine E, David H (1995) Somatic embryogenesis in Pinus caribaea. In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants. Kluwer Academic Publishers, Dordrecht, pp 145–182
  • Dronne S, Label P, Lelu MA (1997) Desiccation decreases abscisic acid content in hybrid larch (Larix 9 leptoeuropea) somatic embryos. Physiol Plant 99:433–438
  • Find JI (1997) Changes in endogenous ABA levels in developing somatic embryos of Norway spruce (Picea abies (L.) Karst.) in relation to maturation medium, dessication and germination. Plant Sci 128:75–83
  • Gupta PK, Holmstrom D (2005) Douglas-fir (Pseudotsuga menziesii). In: Jain SM, Gupta PK (eds) Protocol for somatic embryogenesis in woody plants. Springer, Dordrecht, pp 25–34
  • Hay EI, Charest PJ (1999) Somatic embryo germination and desiccation tolerance in conifers. In: Mohan JS, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants vol. 4. Kluwer Academic Publishers, Dordrecht, pp 61–69
  • Hazubska-Przybył T, Bojarczuk K (2008) Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens ‘Glauca’ and P. breweriana). Acta Soc Bot Pol 77(3):189–199
  • Hoekstra FA, Golovina EA, Buitink J (2001) Mechanism of plant desiccation tolerance. Trends Plant Sci 15(4):431–438
  • Hogberg KA, Bozhkov PV, Gronroos R, von Arnold S (2001) Critical factors affecting ex vitro performance of somatic embryo plants of Picea abies. Scand J For Res 16:295–304
  • Jones NB, Van Staden J (2001) Improved somatic embryo production from embryogenic tissue of Pinus patula. In Vitro Cell Dev Biol Plant 37:543–549
  • Kermode AR, Bewley JD (1985) The role of maturation drying in the transition from seed development to germination. J Exp Bot 36(173):1906–1915
  • Lazzeri PA, Hildebrand DF, Collins G (1987) Soybean somatic embryogenesis: effects of nutritional, physical and chemical factors. Plant Cell Tiss Org Cult 10:209–220
  • Lelu MA, Klimaszewska K, Pflaum G, Bastien C (1995) Effect of maturation duration on desiccation tolerance in hybrid larch (Larix 9 leptoeuropaea Dengler). In Vitro Cell Dev Biol Plant 31:15–20
  • Litvay JD, Verma DC, Johson MA (1985) Influence of loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of wild carrot (Daucus carota L.). Plant Cell Rep 4:325–328
  • Margara J (1977) La multiplication vegetative de la betterave (Beta vulgaris L.) en culture in vitro. CR Acad Sci 285:1041–1044
  • Maruyama TE, Hosoi Y (2012) Post-maturation treatment improves and synchronizes somatic embryo germination of three species of Japanese pines. Plant Cell Tiss Org Cult 110:45–52
  • Park YS (2002) Implementation of somatic embryogenesis in clonal forestry: technical requirements and deployment considerations. Ann For Sci 59:651–656
  • Park YS, Barret JD, Bonga M (1998) Application of somatic embryogenesis in high—value clonal forestry: deployment, genetic control and stability of cryopreserved clones. In Vitro Cell Dev Biol Plant 34:231–239
  • Pond SE, von Aderkas P, Bonga JM (2002) Improving tolerance of somatic embryo of Picea glauca to flash desiccation with a cold treatment (desiccation after cold acclimation). In Vitro Cell Dev Biol Plant 38:334–341
  • Roberts DR, Sutton BCS, Flinn BS (1990) Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity. Can J Bot 68:1086–1090
  • Roberts DR, Lazarof WR, Webster FB (1991) Interaction between maturation and high relative treatments and their effects on germination of sitka spruce somatic embryos. J Plant Physiol 138:1–6
  • Rockland LB (1960) Saturated salt solutions for static control of relative humidity between 5 and 40℃. Anal Chem 32(10):1375–1376
  • Stasolla C, Yeung EC (2003) Recent advances in conifer somatic embryogenesis: improving somatic embryo quality. Plant Cell Tiss Org Cult 74:15–35
  • Szczygieł K, Hazubska-Przybył T, Bojarczuk K (2007) Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix. Acta Soc Bot Pol 7(1):7–15
  • Uddin MR, Keinonen-Mettala K, Dinus RJ (1989) Enhanced germination of Norway spruce somatic embryos. IPC Tech Pap Ser 343:1–11
  • von Arnold S, Hakman I (1988) Regulation of somatic embryo development in Picea abies by abscisic acid (ABA). J Plant Physiol 132:164–169
  • Webster FB, Roberts DR, McInnis SM, Sutton SC (1990) Propagation of interior spruce by somatic embryogenesis. Can J For Res 20:1759–1765
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.