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2011 | 33 | 1 |
Tytuł artykułu

Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
An efficient and rapid plant regeneration system through somatic embryogenesis was developed using 13-week-old zygotic embryos of oil palm (Elaeis guineensis Jacq.) cv. ‘Tenera’. Zygotic embryos were cultured on MS and N6 media supplemented with 2.0 mg L⁻¹ picloram, 2,4-D and dicamba. The highest embryogenic callus formation (32%) was observed on N6 medium with 2,4-D after 3 month culture on callus induction medium. Somatic embryos were continuously formed from nodular calli on embryo maturation medium [N6 + 0.1 mg L⁻¹ 2,4-D, 0.16 g L⁻¹ putrescine, 0.5 g L⁻¹ casein amino acids and 2.0 g L⁻¹ activated charcoal(AC)] for 3–5 months. Histological analysis confirmed that embryo development occurred via somatic embryogenesis. For plant regeneration, modified N6 medium (MN6) with AC (0.5 g L⁻¹) without growth regulators, induced both shoot and root formation simultaneously with the highest regeneration rate of 56%. This combined shoot and root induction protocol shortened the culture time to 9–12 months. Furthermore, after acclimatization, more than 85% of transferred plants from our protocol developed successfully in the soil.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
33
Numer
1
Opis fizyczny
p.123-128,fig.,ref.
Twórcy
autor
  • Tropical Agriculture Program, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand
  • Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand
  • Genome Institute, BIOTEC, Klong Luang, Pathumthani 12120, Thailand
  • Department of Genetics, Faculty of Science, Kasetsart University, Bangkhen Campus, Bangkok 10900, Thailand
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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