Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis

• Autorzy: Thuzar M. , Vanavichit A. , Tragoonrung S. , Jantasuriyarat C.
• Języki publikacji: EN

Abstrakty

EN

An efficient and rapid plant regeneration system through somatic embryogenesis was developed using 13-week-old zygotic embryos of oil palm (Elaeis guineensis Jacq.) cv. ‘Tenera’. Zygotic embryos were cultured on MS and N6 media supplemented with 2.0 mg L⁻¹ picloram, 2,4-D and dicamba. The highest embryogenic callus formation (32%) was observed on N6 medium with 2,4-D after 3 month culture on callus induction medium. Somatic embryos were continuously formed from nodular calli on embryo maturation medium [N6 + 0.1 mg L⁻¹ 2,4-D, 0.16 g L⁻¹ putrescine, 0.5 g L⁻¹ casein amino acids and 2.0 g L⁻¹ activated charcoal(AC)] for 3–5 months. Histological analysis confirmed that embryo development occurred via somatic embryogenesis. For plant regeneration, modified N6 medium (MN6) with AC (0.5 g L⁻¹) without growth regulators, induced both shoot and root formation simultaneously with the highest regeneration rate of 56%. This combined shoot and root induction protocol shortened the culture time to 9–12 months. Furthermore, after acclimatization, more than 85% of transferred plants from our protocol developed successfully in the soil.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 1
• Opis fizyczny: p.123-128,fig.,ref.

Twórcy

autor

Thuzar M.

• Tropical Agriculture Program, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand

autor

Vanavichit A.

• Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand

autor

Tragoonrung S.

• Genome Institute, BIOTEC, Klong Luang, Pathumthani 12120, Thailand

autor

Jantasuriyarat C.

• Department of Genetics, Faculty of Science, Kasetsart University, Bangkhen Campus, Bangkok 10900, Thailand

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Identyfikatory

DOI

10.1007/s11738-010-0526-6