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Tytuł artykułu

Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis

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An efficient and rapid plant regeneration system through somatic embryogenesis was developed using 13-week-old zygotic embryos of oil palm (Elaeis guineensis Jacq.) cv. ‘Tenera’. Zygotic embryos were cultured on MS and N6 media supplemented with 2.0 mg L⁻¹ picloram, 2,4-D and dicamba. The highest embryogenic callus formation (32%) was observed on N6 medium with 2,4-D after 3 month culture on callus induction medium. Somatic embryos were continuously formed from nodular calli on embryo maturation medium [N6 + 0.1 mg L⁻¹ 2,4-D, 0.16 g L⁻¹ putrescine, 0.5 g L⁻¹ casein amino acids and 2.0 g L⁻¹ activated charcoal(AC)] for 3–5 months. Histological analysis confirmed that embryo development occurred via somatic embryogenesis. For plant regeneration, modified N6 medium (MN6) with AC (0.5 g L⁻¹) without growth regulators, induced both shoot and root formation simultaneously with the highest regeneration rate of 56%. This combined shoot and root induction protocol shortened the culture time to 9–12 months. Furthermore, after acclimatization, more than 85% of transferred plants from our protocol developed successfully in the soil.
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  • Tropical Agriculture Program, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand
  • Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand
  • Genome Institute, BIOTEC, Klong Luang, Pathumthani 12120, Thailand
  • Department of Genetics, Faculty of Science, Kasetsart University, Bangkhen Campus, Bangkok 10900, Thailand
  • Abdullah R, Zainal A, Heng WY, Li LC, Beng YC, Phing LM, Sirajuddin SA, Ping WYS, Joseph JL, Jusoh SA, Muad MR, Huey YL (2005) Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies. Electron J of Biotechnol 8 (1) (ISSN: 0717-3458)
  • Bertossi AF, Noirot M, Duval Y (1999) BA enhances the germination of oil palm somatic embryos derived from embryogenic suspension cultures. Plant Cell Tissue Organ Cult 56:53–57
  • Besse I, Verdeil JL, Duval Y, Sotta B, Maldiney R, Miginia E (1992) Oil palm (Elaeis guineensis Jacq.) clonal fidelity: endogenous cytokinins and indolacetic acid in embryogenic callus cultures. J Exp Bot 43:983–989
  • Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668
  • Cochard B, Durand-Gasselin T, Amblard P, Konan EK, Gogor S (2000) Performance of adult oil palm clones. In: Emerging technologies and opportunities in the next millennium. Agriculture conference: proceedings of 1999 PORIM International Palm Oil Congress (PIPOC 1999), Kuala Lumpur, Malaysia, pp. 53–64
  • Corley RHV, Tinker PB (2003) The oil palm, 4th edn. Blackwell, Oxford, p 202
  • Eeuwens CJ, Lord S, Donough CR, Rao V, Vallejo G, Nelson S (2002) Effects of tissue culture conditions during embryoid multiplication on the incidence of ‘‘mantled’’ flowering in clonally propagated oil palm. Plant Cell Tissue Organ Cult 70:311–323
  • Fehér A, Pasternak TP, Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue Organ Cult 74:201–228
  • IRRI 2004, IRRISTAT for Windows Version 4. Tutorial Manual. Biometrics and Bioinformatics Unit, IRRI, Los Baños, Philippines
  • Konan EK, Kouadio JY, Flori A, Durand-Gasselin T, Rival A (2007) Evidence for an interaction effect during in vitro rooting of oil palm (Elaeis guineensis Jacq.) somatic embryo-derived plantlets. In Vitro Cell Dev Biol Plant 43:456–466
  • Kothari-Chajer A, Sharma M, Kachhwaha S, Kothari SL (2008) Micronutrient optimization results into highly improved in vitro plant regeneration in kodo (Paspalum scrobiculatum L.) and finger millets (Eleusine coracana L.). Plant Cell Tissue Organ Cult 94:105–112
  • Liu L, Fan X, Zhang J, Yan M, Bao M (2009) Long-term cultured callus and the effect factor of high-frequency plantlet regeneration and somatic embryogenesis maintenance in Zoysia japonica. In Vitro Cell Dev Biol Plant 45:673–680
  • Mielke S (1991) Economic prospects for oilseeds, oils and fats toward the 21st century. In: Basiron Y and Ibrahim A (ed) The proceedings of the 1991 PORIM International Palm Oil Conference. Module IV: promotion and marketing. September 9–14, Palm Oil Research Institute of Malaysia, Kuala Lumpur, pp 1-16
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Rajesh MK, Radha AKE, Parthasarathy VA (2003) Plant regeneration from embryo-derived callus of oil palm; the effect of exogenous polyamines. Plant Cell Tissue Organ Cult 75:41–47
  • Sass JE (1958) Botanical microtechnique, 3rd edn. Iowa State University Press, Ames
  • Soh AC (1986) Expected yield increase with selected oil palm clones from current seedling material. Oleagineux 41(2):51–56
  • Teixeira JB, Söndahl MR, Kirby EG (1993) Somatic embryogenesis from immature zygotic embryos of oil palm. Plant Cell Tissue Organ Cult 34:227–233
  • Teixeira JB, Söndahl MR, Kirby EG (1994) Somatic embryogenesis from immature inflorescences of oil palm. Plant Cell Rep 13(5):247–250
  • Williams EG, Maheswaran G (1986) Somatic embryogenesis: Factors influencing coordinated behavior of cells as an embryogenic group. Ann Bot 57:443–462
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