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2015 | 37 | 09 |
Tytuł artykułu

NtGRAS-R1, a topping responsive transcription regulator in tobacco roots

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Języki publikacji
EN
Abstrakty
EN
Topping, the critical cultural practice, is a kind of damage occurred tobacco shoot, which can obviously promote roots growth. Up to now, the mechanism regulating roots growth after topping is still unclear. In our previous works, miR171d was identified as a major topping responsive miRNA in tobacco roots, and its target gene was predicted to belong to the GRAS gene family. In the present study, NtGRAS-R1, a novel GRAS transcription regulator, was firstly cloned from tobacco roots. NtGRAS-R1 contained an open reading frame of 1527 bp encoding a 508-amino acids protein, and its molecular mass and isoelectric point was 56.199 KD and 5.24, respectively. GRAS proteins in tobacco can be grouped into nine subfamilies, including HAM, SCR, LAS, DLT, DELLA, SCL, LISCL, SHR and PAT1. NtGRAS-R1 is highly homologous with NtGRAS44 and belongs to HAM subfamily. The expression of NtGRAS-R1 can be detected in tobacco roots, leaves and stems, and its expression level was highest in tobacco roots. The analysis of transgenic tobacco showed that NtGRAS-R1 was involved in several biology processes, such as rooting, germination and root development, which will be helpful to explore the roles of NtGRAS-R1 in response to tobacco topping.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
09
Opis fizyczny
Article: 191 [8 p.], fig.,ref.
Twórcy
autor
  • College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China
autor
  • College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China
autor
  • Henan Science and Technology Exchange Center with Foreign Countries, Zhengzhou, 450002, Henan, China
autor
  • College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China
autor
  • College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China
autor
  • College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China
autor
  • College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China
Bibliografia
  • Ariel F, Brault-Hernandez M, Laffont C, Huault E, Brault M, Plet J, Moison M, Blanchet S, Ichanté JL, Chabaud M, Carrere S, Crespi M, Chan RL, Frugier F (2012) Two direct targets of cytokinin signaling regulate symbiotic nodulation in Medicago truncatula. Plant Cell 24:3838–3852
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-0c26ed25-f1cf-483b-83d9-81f8aa534324
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