Effect of chronic ethanol treatment and the abstinence period on the ethanol elimination by isolated rat liver lesioned by carbon tetrachloride

• Autorzy: Rajtar G. , Kleinrok Z.
• Języki publikacji: EN

Abstrakty

EN

Rajtar G. and Kleinrok Z.: Effect of chronic ethanol treatment and the abstinence period on the ethanol elimination by isolated rat liver lesioned by carbon terachloride Acta Physiol. Pol. The study was carried out on perfused livers isolated from rats receiving ethanol (EtOH) as their only drinking fluid for the period of 4 weeks. Twelve, 24, 72 and 120 hours after EtOH withdrawal the livers were isolated and perfused with 100 ml of perfusion mixture with addition of EtOH (0.2% final concentration). After 12 hours of EtOH withdrawal acceleration of the EtOH elimination from perfusate was observed. It returned to the control level after 24 hours, but after 72 and 120 hours of abstinence the rate of EtOH elimination from perfusate was found to diminish. CC1₄ injected to the rats in doses of 2 and 5 mmoles/kg once a week for the period of 4 or 8 weeks, resulted in decreased EtOH elimination from the perfusate. In the EtOH-drinking group previously treated with CC1₄ we found that irrespective of the time of EtOH withdrawal, EtOH elimination did not differ from that in the respective CC1₄ treated group, only 12 hours after its withdrawal EtOH elimination was decreased in livers injured with CC1₄ in dose of 5 mmoles/kg.

Słowa kluczowe

EN

chronic treatment; ethanol treatment; abstinence period; ethanol elimination; rat; liver; carbon tetrachloride

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1990
• Tom: 41
• Numer: 7
• Opis fizyczny: p.8-13,fig.,ref.

Twórcy

autor

Rajtar G.

• Department of Pharmacology, Medical Academy, Jaczewskiego 8, 20-090 Lublin, Poland

autor

Kleinrok Z.

• Department of Pharmacology, Medical Academy, Jaczewskiego 8, 20-090 Lublin, Poland

Bibliografia

• 1. Boobis A. R., Davies D. S. (1984): Multiple forms of human cytochrome P-450. Biochem. Soc. Trans., 12, 78-80.
• 2. Glende E. A., Pushpendran С. K. (1986): Activation of phospholipase A₂ by carbon tetrachloride in isolated rat hepatocytes. Bioch. Pharmacol., 35, 3301-3307.
• 3. Hasumura Y., Teschke R., Lieber C. S. (1974): Increased carbon tetrachloride hepatotoxicity and its mechanism after chronic ethanol consumption. Gastroenterology, 66, 415-422.
• 4. Head B., Moody O. E., Woo C. H., Smuckler E. A. (1981): Alterations of specific forms of cytochrome P-450 in rat liver during acute carbon tetrachloride intoxication. Toxicol. Appl. Pharmacol., 61, 286-295.
• 5. Iseri O. A., Lieber C. S., Gotelieb L. S. (1966): The ultrastructure of fatty liver induced by prolonged ethanol ingestion. Am. J. Pathol., 48, 535-555.
• 6. Keiding S., Christiansen N. J., Damgard S. E., Dejgard A., Iversen H. L., Jacobsen A., Johansen S., Linquist F., Rubinstein E., Winkler K. (1983): Ethanol metabolism in heavy drinkers after massive and moderate alcohol intake. Bioch. Pharmacol., 32, 3097-3102.
• 7. Miller L. L. (1973): Technique of isolated rat liver perfusion, in: Isolated liver perfusion and its applications, ed. by I Bartošek, A. Guaitani and L. L. Miller, Raven Press, New York p. 10-52.
• 8. Pikkarainen P., Lieber C. S. (1980): Concentration dependency of ethanol elimination rates in baboons: Effect of chronic ethanol conusmption. Alcoholism: Clin. Exp. Res., 4, 40-43.
• 9. Rajtar G. (1988): Influence of chronic ethanol treatment and the abstinence period on the ethanol elimination by the isolated perfused rat liver. Acta Physiol. Pol., 39, 276-280.
• 10. Recknagel R. O. (1983): A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci., 33, 401-408.
• 11. Slaspuro M. P., Shaw S., Jayatilleke E. (1981): Attenuation of ethanol-induced hepatic redox change after chronic alcohol consumption: Mechanism and metabolic conseqeunces. Hepatology, 1, 33-38.
• 12. Sasame H. A., Castro J. A., Gillette J. R. (1968): Studies on the destruction of liver microsomal cytochrome P-450 by carbon tetrachloride administration. Bioch. Pharmacol., 17, 1759-1768.
• 13. Shaffer M., Stroupe S. D. (1983): A general method for routine clinical chemistry on the Abbot TDX analyzer. Clin. Chern., 29/6, 1251.
• 14. Shigeta Y., Nomura F., lida S. (1984): Ethanol metabolism in vivo by the microsomal ethanol oxidazing system in deermice lacking alcohol dehydrogenase (ADH). Bioch Pharmacol., 33, 807-814.
• 15. Stiles D., Batsakis J. G., Kremers B., Briere R. O. (1966): The evaluation of ethanol measurements with alcohol deyhdrogenase. Am. J. Clin. Pathol., 46, 608-614.
• 16. Tobon F., Mezey E. (1971): Effect of ethanol administration on hepatic ethanol and drug-metabolizing enzymes and on rates of ethanol degradation J. Lab. Clin. Med., 77, 110-121.