Cytotoxic effects of zinc on cholinergic and astroglial cells

• Autorzy: Dys A. , Ronowska A. , Klimaszewska-Lata J. , Gul-Hinc S. , Bielarczyk H. , Szutowicz A.
• Języki publikacji: EN

Abstrakty

EN

Zinc excess in the synaptic cleft may be one of pathologic signals triggering chronic neurodegenerative events. The aim of this work was to find relationships between Zn accumulation and integrity of cholinergic and astroglial cells. Exposition of cAMP/RA-differentiated (DC) and nondifferentiated cells (NC) cholinergic SN56 neuroblastoma and astroglial C6 cells to Zn yielded its concentration dependent accumulation. It caused inhibition of pyruvate dehydrogenase, aconitase and ketoglutarate dehydrogenase activities. Zn accumulation caused concentration-dependent death of both neuronal and astroglial cells. After 24 h exposition of SN56 cells to 0.15 mM Zn their death rates were equal to 35 and 50% for NC and DC at cation levels equal to 4.0 and 5.5 nmol/mg protein, respectively. In the same conditions, the death rates of astroglial NC and DC were close to 1–2% only, at intracellular Zn levels of 1.6 and 2.1 nmol/ mg protein, respectively. Higher about 0.25 mM Zn levels were required to evoke death rates of astroglial cells, similar to those seen in neuronal cells. In such conditions Zn levels in astroglia were about 6.4 and 27.0 nmol/mg protein, respectively. Such differential sensitivity of astroglial and neuronal cholinergic NC and DC to Zn may be due to respective differences in densities of ZnT1 transporters in their plasma membranes. Supported by M.S&H.E. project IP2010035370 and GUMed fund ST57.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.62

Twórcy

autor

Dys A.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Ronowska A.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Klimaszewska-Lata J.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Gul-Hinc S.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Bielarczyk H.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Szutowicz A.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland