BDNF modifies hippocampal KCC2 and NKCC1 expression in a temporal lobe epilepsy model

• Autorzy: Eftekhari S. , Mehrabi S. , Soleimani M. , Hassanzadeh G. , Shahrokhi A. , Mostafavi H. , Hayat P. , Barati M. , Mehdizadeh H. , Rahmanzadeh R. , Mostafavi H. , Barati M. , Mehdizadeh H. , Rahmanzadeh R. , Hadjighassem M. R. , Joghataei M. T.
• Języki publikacji: EN

Abstrakty

EN

Excitatory GABA actions, induced by altered expression of chloride transporters (KCC2/NKCC1), can contribute to seizure generation in temporal lobe epilepsy. In the present study, we evaluated whether BDNF administration can affect KCC2/ NKCC1 expression, ictogenesis and behavioral alterations in this paradigm. Status epilepticus was induced in male rats with pilocarpine, followed by a treatment of either a single high dose or multiple injections of BDNF during the latent phase of temporal lobe epilepsy. Chloride transporters expression, spontaneous recurrent seizures, and hyperexcitability post-seizural behaviors were evaluated after treatment. NKCC1 protein expression was markedly upregulated, whereas that of KCC2 was significantly downregulated in epileptic hippocampi compared to intact controls. Application of BDNF (both single high dose and multiple injections) increased KCC2 expression in epileptic hippocampi, while NKCC1 expression was downregulated exclusively by the single high dose injection of BDNF. Development of spontaneous recurrent seizures was delayed but not prevented by the treatment, and hyperexcitability behaviors were ameliorated for a short period of time. To prevent GABA-A mediated depolarization and design appropriate treatment strategies for temporal lobe epilepsy, chloride transporters can be considered as a target. Future studies are warranted to investigate any possible therapeutic effects of BDNF via altering chloride transporters expression.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 3
• Opis fizyczny: p.176-187,fig.,ref.

Twórcy

autor

Eftekhari S.

• Department of Neuroscience, School of Advanced Technologies in Medicine
• Division of Neuroscience, Cellular and Molecular Research Center

autor

Mehrabi S.

• Division of Neuroscience, Cellular and Molecular Research Center
• Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Soleimani M.

• Division of Neuroscience, Cellular and Molecular Research Center
• Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

autor

Hassanzadeh G.

• Department of Anatomy, School of Medicine
• Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Shahrokhi A.

• Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Mostafavi H.

• Department of Physiology and Pharmacology, Zanjan University of Medical Sciences, Zanjan, Iran

autor

Hayat P.

• Division of Neuroscience, Cellular and Molecular Research Center

autor

Barati M.

• Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

autor

Mehdizadeh H.

• Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Rahmanzadeh R.

• Division of Neuroscience, Cellular and Molecular Research Center

autor

Mostafavi H.

• Department of Physiology and Pharmacology, Zanjan University of Medical Sciences, Zanjan, Iran

autor

Barati M.

• Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

autor

Mehdizadeh H.

• Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

autor

Rahmanzadeh R.

• Division of Neuroscience, Cellular and Molecular Research Center

autor

Hadjighassem M. R.

• Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
• Brain and Spinal Cord Injury Research Centre, Imam Khomeini Hospital, Tehran University of Medical sciences, Tehran, Iran

autor

Joghataei M. T.

• Department of Neuroscience, School of Advanced Technologies in Medicine
• Division of Neuroscience, Cellular and Molecular Research Center
• Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

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