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2014 | 17 | 2 |
Tytuł artykułu

Bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus)

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Free-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
17
Numer
2
Opis fizyczny
p.315-319,fig.,ref.
Twórcy
  • Department of Epizootiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Epizootiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
  • Department of Epizootiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
autor
  • Department of Epizootiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
Bibliografia
  • Aschfalk A, Kemper N, Arnemo JM, Veiberg V, Rosef O, Neubauer H (2008) Prevalence of Yersinia species in healthy free-ranging red deer (Cervus elaphus) in Norway. Vet Rec 163: 27-28.
  • Bancerz-Kisiel A, Szczerba-Turek A, Platt-Samoraj A, Socha P, Szweda W (2009) Application of multiplex PCR for the evaluation of the occurrence of ail, ystA, and ystB genes in Yersinia enterocolitica strains isolated from wild boars (Sus scrofa). Bull Vet Inst Pulawy 53: 351-355.
  • Bancerz-Kisiel A, Szczerba-Turek A, Lipczyńska K, Stenzel T, Szweda W (2012) Bioserotypes and virulence markers of Yersinia enterocolitica strains isolated from mallards (Anas platyrhynchos) and pheasants (Phasianus colchicus). J Food Prot 75: 2219-2222.
  • Bottone EJ (1997) Yersinia enterocolitica: the charisma continues. Clin Microbiol Rev 10: 257-276.
  • French E, Rodriguez-Palacios A, LeJeune JT (2010) Enteric bacterial pathogens with zoonotic potential isolated from farm-raised deer. Foodborne Pathog Dis 7: 1031-1037.
  • Henderson TG (1984) The isolation of Yersinia sp. from feral and farmed deer faeces. N Z Vet J 32: 88-90.
  • Joutsen S, Sarno E, Fredriksson-Ahomaa M, Cernela N, Stephan R (2013) Pathogenic Yersinia enterocolitica O:3 isolated from a hunted wild alpine ibex. Epidemiol Infect 141: 612-617.
  • Koronkiewicz A, Daczkowska-Kozon E, Markiewicz K, Wojciechowska A, Żmuda E, Dąbrowski W (2004) Game animals as carriers of enteric pathogens. Folia Universitatis Agriculturae Stetinensis, Scientia Alimentaria 3: 79-84.
  • Magnino S, Frasnelli M, Fabbi M, Bianchi A, Zanoni MG, Merialdi G, Pacciarini ML, Gaffuri A (2011) The monitoring of selected zoonotic diseases of wildlife in Lombardy and Emilia-Romagna, northern Italy. In: Paulsen P, Bauer A, Vodnansky M, Winkelmayer R, Smulders FJM (eds) Game meat hygiene in focus: microbiology, epidemiology, risk analysis and quality assurance. Wageningen Academic Publishers, Wageningen, The Netherlands, pp 223-245.
  • McNally A, Cheasty T, Fearnley C, Dalziel RW, Paiba GA, Manning G, Newell DG (2004) Comparison of the biotypes of Yersinia enterocolitica isolated from pigs, cattle and sheep at slaughter and from humans with yersiniosis in Great Britain during 1999-2000. Lett Appl Microbiol 39: 103-108.
  • Pagano A, Nardi G, Bonaccorso C, Falbo V, Passi C, Sanguinetti V, Mantovani A (1985) Faecal bacteria of wild ruminants and the alpine marmot. Vet Res Commun 9: 227-232.
  • Platt-Samoraj A, Bancerz-Kisiel A, Szweda W (2006) Yersinia enterocolitica pathogenicity and the significance of biotype 1A in the pathogenesis of yersiniosis. Med Weter 62: 1113-1115.
  • Shayegani M, Stone WB, DeForge I, Root T, Parsons LM, Maupin P (1986) Yersinia enterocolitica and related species isolated from wildlife in New York state. Appl Environ Microbiol 52: 420-424.
  • Singh I, Bhatnagar S, Virdi JS (2003) Isolation and characterization of Yersinia enterocolitica from diarrhoeic human subjects and other sources. Curr Sci 84: 1353-1355.
  • Singh I, Virdi JS (2004) Production of Yersinia stable toxin (YST) and distribution of yst genes in biotype 1A strains of Yersinia enterocolitica. J Med Microbiol 53: 1065-1068.
  • Singh I, Virdi JS (2005) Interaction of Yersinia enterocolitica biotype 1A strains of diverse origin with cultured cells in vitro. Jpn J Infect Dis 58: 31-33.
  • Tennant SM, Skinner NA, Joe A, Robins-Browne RM (2005) Homologues of insecticidal toxin complex genes in Yersinia enterocolitica biotype 1A and their contribution to virulence. Infect Immun 73: 6860-6867.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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