Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2018 | 27 | 2 |
Tytuł artykułu

Detection of genetically modified food in digesta and organs of rats fed transgenic potato

Warianty tytułu
Języki publikacji
Transgenic potato plants harbouring cry1Ab gene, resistant to potato tuber moth, were used to examine the persistence of recombinant DNA and proteins in the digesta, gastrointestinal tract (GIT) tissues and visceral organs of rats in a 90-day feeding trial. Native plant DNA (chloroplastic gene, cp) and fragments of cry1Ab, nptII and nos promoter were tracked in rat organs and tissues by polymerase chain reaction (PCR). Although complete sequences (1200 bp) of cry1Ab gene and its decreased size (400 bp) were detected in the rat diets, the presence of these sequences was not confirmed by PCR analysis in the GIT contents of rats fed diets containing transgenic potato. Moreover, the 400 bp sequence of the nptII gene and 300 bp of the nos promoter were detected in the caecum, stomach and rectum contents of some rats fed transgenic potato; however, these sequences were not detected in all rats. Immunoassay showed that Cry1Ab protein is detectable in the GIT contents of rats fed transgenic potato, but quantitative assay by ELISA confirmed that the Cry1Ab protein was partially degraded (80.3–83.7% digestibility) after passing through the GIT. Similarly, ELISA assay of NPTII showed that the protein can be digested (76.8–84.36% digestibility) in the GIT. So, although ingested native and recombinant DNA and protein did not totally degrade in the GIT, it can be indicated that there is no need for concern about the effects of recombinant cry1Ab and nptII genes and their products on animals and humans.
Słowa kluczowe
Opis fizyczny
  • Plant Genetic Engineering and Biosafety Department, Agricultural Research Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran
  • Department of Agricultural Biotechnology, Science and Research Branch, slamic Azad University, 1584743311 Tehran, Iran
  • Plant Genetic Engineering and Biosafety Department, Agricultural Research Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran
  • Plant Genetic Engineering and Biosafety Department, Agricultural Research Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran
  • Plant Genetic Engineering and Biosafety Department, Agricultural Research Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran
  • Breyer D., Kopertekh L., Reheul D., 2014. Alternatives to antibiotic resistance marker genes for in vitro selection of genetically modified plants – scientific developments, current use, operational access and biosafety considerations. Crit. Rev. Plant Sci. 33, 286–330,
  • Broll B., Zagon J., Butschke A., Leffke A., Spiegelberg A., Böhme H., Flachowsky G., 2005. The fate of DNA of transgenic inulin synthesizing potatoes in pigs. J. Anim. Feed. Sci. 14, Suppl. 1, 337–340,
  • Buzoianu S.G., Walsh M.C., Rea M.C. et al., 2012. Effects of feeding Bt maize to sows during gestation and lactation on maternal and offspring immunity and fate of transgenic material. PLoS ONE 7, e47851,
  • Chowdhury E.H., Kuribara H., Hino A., Sultana P., Mikami O., Shimada N., Guruge K.S., Saito M., Nakajima Y., 2003. Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11. J. Anim. Sci. 81, 2546–2551,
  • Devos Y., Aguilera J., Diveki Z., Gomes A., Liu Y., Paoletti C., du Jardin P., Herman J., Perry J.N., Waigmann E., 2014. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead. Transgenic Res., 23, 1–25,
  • Doerfler W., 2000. Foreign DNA in Mammalian Systems. WileyVCH Verlag GmbH, Wennheim (Germany),
  • Dona A., Arvanitoyannis I.S., 2009. Health risks of genetically modified foods. Crit. Rev. Food Sci. Nutr. 49, 164–175,
  • Duggan P.S., Chambers P.A., Heritage J., Forbes J.M., 2000. Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid and silage effluent. FEMS Microbiol. Lett. 191, 71–77,
  • Duggan P.S., Chambers P.A., Heritage J., Forbes J.M., 2003. Fate of genetically modified maize DNA in the oral cavity and rumen of sheep. Br. J. Nut. 89, 159–166,
  • El-Sanhoty R.M.E.S.S., El-Maged A.D.A., Ramadan M.F., 2006. Safety assessment of genetically modified potato Spunta: degradation of DNA in gastrointestinal tract and carryover to rat organs. J. Food Biochem. 30, 556–578,
  • European Food Safety Authority (EFSA), 2004. Opinion of the Scientific Panel on Genetically Modified Organisms on the use of antibiotic resistance genes as marker genes in genetically modified plants. EFSA J. 2, 48,
  • European Food Safety Authority (EFSA), 2007. Statement on the safe use of the nptII antibiotic resistance marker gene in genetically modified plants by the Scientific Panel on Genetically Modified Organisms. EFSA J. 5, 742,
  • European Food Safety Authority (EFSA), 2009. Consolidated presentation of the joint Scientific Opinion of the GMO and BIOHAZ Panels on the “Use of Antibiotic Resistance Genes as Marker Genes in Genetically Modified Plants” and the Scientific Opinion of the GMO Panel on “Consequences of the Opinion on the Use of Antibiotic Resistance Genes as Marker Genes in Genetically Modified Plants on Previous EFSA Assessments of Individual GM Plants”. EFSA J. 7, 1108,
  • Fuchs R.L., Ream J.E., Hammond B.G., Naylor M.W., Leimgruber R.M., Berberich S.A., 1993. Safety assessment of the neomycin phosphotransferase II (NPTII) protein. Nat. Biotechnol. 11, 1543–1547,
  • Gallori E., Bazzicalupo M., Dal Canto L., Fani R., Nannipieri P., Vettori C., Stotozky G., 1994. Transformation of Bacillus subtilis by DNA bound on clay in non-sterile soil. FEMS Microbiol. Ecol. 15, 119–126,
  • Ghasimi Hagh Z., Rahnama H., Panahandeh J., Rouz B.B.K., Jafari K.M.A., Mahna N., 2009. Green-tissue-specific, C4-PEPC-promoter-driven expression of Cry1Ab makes transgenic potato plants resistant to tuber moth (Phthorimaea operculella, Zeller). Plant Cell Rep. 28, 1869–1879,
  • Grønsberg I.M., Nordgård L., Fenton K., Hegge B., Nielsen K.M., Bardocz S., Pusztai A., Traavik T., 2011. Uptake and organ distribution of feed introduced plasmid DNA in growing or pregnant rats. Food Nutr. Sci. 2, 377–386,
  • He X.Y., Tang M.Z., Luo Y.B., Li X., Cao S.S., Yu J.J., Delaney B., Huang K.L., 2009. A 90-day toxicology study of transgenic lysine-rich maize grain (Y642) in Sprague-Dawley rats. Food Chem. Toxicol. 47, 425–432,
  • Koch M.S., Ward J.M., Levine S.L., Baum J.A., Vicini J.L., Hammond B.G., 2015. The food and environmental safety of Bt crops. Front. Plant Sci. 6, 283,
  • Kosieradzka I., Vasko V., Szwacka M., Przybysz A., Fiedorowicz Sz., 2010. Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats. J. Anim. Feed Sci. 19, 307–315,
  • Kuiper H.A., Kleter G.A., Noteborn H.P.J.M., Kok E.J., 2001. Assessment of the food safety issues related to genetically modified foods. Plant J. 27, 503–528,
  • Mazza R., Soave M., Morlacchini M., Piva G., Marocco A., 2005. Assessing the transfer of genetically modified DNA from feed to animal tissues. Transgenic Res. 14, 775–784,
  • National Research Council (NRC), 1995. Nutrient Requirements of Laboratory Animals. 4th Revised Edition. The National Academies Press. Washington, DC (USA), pp. 11–79,
  • Nielsen K.M., Bones A.M., Smalla K., van Elsas J.D., 1998. Horizontal gene transfer from transgenic plants to terrestrial bacteria – a rare event? FEMS Microbiol. Rev. 22, 79–103,
  • Nielsen K.M., Daffonchio D., 2010. Unintended Horizontal Transfer of Recombinant DNA. TWN Biotechnology and Biosafety Series No. 13. TWN. Penang (Malaysia)
  • Ramessar K., Peremarti A., Gómez-Galera S., Naqvi S., Moralejo M., Muñoz P., Capell T., Christou P., 2007. Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics. Transgenic Res. 16, 261–280,
  • Reuter T., Aulrich K., 2003. Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feed-ingested foreign DNA in pig bodies. Eur. Food Res. Technol. 216, 185–192,
  • Sharma R., Damgaard D., Alexander T.W., Dugan M.E.R., Aalhus J.L., Stanford K., McAllister T.A., 2006. Detection of transgenic and endogenous plant DNA in digesta and tissues of sheep and pigs fed Roundup Ready canola meal. J. Agric. Food Chem. 54, 1699–1709,
  • Snell C., Bernheim A., Bergé J.-B., Kuntz M., Pascal G., Paris A., Ricroch A.E., 2012. Assessment of the health impact of GM plant diets in long-term and multigenerational animal feeding trials: A literature review. Food Chem. Toxicol. 50, 1134–1148,
  • Swiatkiewicz S., Swiatkiewicz M., Arczewska-Wlosek A., Jozefiak D., 2014. Genetically modified feeds and their effect on the metabolic parameters of food-producing animals: A review of recent studies. Anim. Feed Sci. Technol. 198, 1–19,
  • Świątkiewicz S., Koreleski J., Arczewska-Włosek A., Świątkiewicz M., Twardowska M., Markowski J., Mazur M., Sieradzki Z., Kwiatek K., 2011. Detection of transgenic DNA from Bt maize and herbicide tolerant soybean meal in tissues, eggs and contents of segments of digestive tract in laying hens fed diets containing genetically modified plants. Ann. Anim. Sci. 11, 413–424
  • Tudisco R., Lombardi P., Bovera F., d’Angelo D., Cutrignelli M.I., Mastellone V., Terzi V., Avallone L., Infascelli F., 2006. Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis. Anim. Sci. 82, 193–199,
  • Walsh M.C., Buzoianu S.G., Gardiner G.E., Rea M.C., Gelencsér E., Jánosi A., Epstein M.M., Ross R.P., Lawlor P.G., 2011. Fate of transgenic DNA from orally administered Bt MON810 maize and effects on immune response and growth in pigs. PLoS ONE 6, e27177,
  • Watson J.C., Thompson W.F., 1988. Purification and restriction endonuclease analysis of plant nuclear DNA. In: A. Weissbach, H. Weissbach (Editors). Methods for Plant Molecular Biology. Academic Press, San Diego, CA (USA),
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.