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2011 | 29 | 4 |
Tytuł artykułu

Androgen receptor inhibitor stimulates telomerase activity of pig granulosa cells in vitro

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the study was to investigate the effect of testosterone (T) and dihydrotestosterone (DHT) applied alone or in combination with antiandrogen (flutamide) on proliferation, progesterone secretion and telomerase activity (TA) of pig granulosa cells (GC) derived from small (1-3 mm; SFGC) and large (5-7 mm; LF-GC) ovarian follicles. Cells were treated with investigated factors for 48 h. 5α-DHT stimulated (P<0.05-0.01) proliferation of SF and LF granulosa cells. Flutamide applied individually and in a combination with testosterone and DHT stimulated (P<0.05-0.01) proliferation of pig GC from small and large antral follicles. Flutamide had no effect on progesterone synthesis in small as well as in large follicle GC. Antiandrogen applied individually and in a combination with testosterone and DHT enhanced (P<0.05-0.01) telomerase actitvity in SF- and LF-GC. The results of the study suggest the involvement of androgen receptor in telomerase activity regulation in pig GC and a link between telomerase and the proliferation status of GC.
Wydawca
-
Rocznik
Tom
29
Numer
4
Opis fizyczny
p.313-323,fig.,ref.
Twórcy
  • Department of Biology of Reproduction, Institute of Animal Science, Pratelstvi 815, 104 00 Prague-Uhrineves, Czech Republic
autor
Bibliografia
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  • CARDENAS H., POPE W.F., 2002 – Androgen receptor and follicle-stimulating hormone receptor in the pig ovary during the follicular phase of the estrus cycle. Molecular Reproduction and Development 62, 92-98.
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  • CHRONOWSKA E., TOMANEK M., KOTT T., 2010 – Effect of antioestrogens on proliferation,differentiation and telomerase activity of pig granulosa cells in vitro. Journal of Animal and Feed Sciences 19, 577-589.
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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