Coping with iron limitation: a metabolomic study of Synechocystis sp. PCC 6803

• Autorzy: Rivas-Ubach A. , Poret-Peterson A.T. , Penuelas J. , Sardans J. , Perez-Trujillo M. , Legido-Quigley C. , Oravec M. , Urban O. , Elser J.J.
• Języki publikacji: EN

Abstrakty

EN

Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N₂-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA:DNA, C, N, and P, and higher ratios of protein:RNA, C:N, C:P, and N:P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C:P and N:P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn, and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. In addition, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular, and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2018
• Tom: 40
• Numer: 02
• Opis fizyczny: Article 28 [13p.], fig.,ref.

Twórcy

autor

Rivas-Ubach A.

• Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 3335 Innovation Boulevard, Richland, WA 99354, USA
• CREAF, Cerdanyola del Valles, 08913 Catalonia, Spain

autor

Poret-Peterson A.T.

• School of Earth and Space Exploration, Arizona State University, Tempe, AZ 85287, USA
• USDA‑ARS, Crops Pathology and Genetics Research Unit, University of California, Davis, CA 95616, USA

autor

Penuelas J.

• CREAF, Cerdanyola del Valles, 08913 Catalonia, Spain
• CSIC, Global Ecology Unit CREAF-CEAB-CSIC-UAB, Cerdanyola del Valles, 08913 Catalonia, Spain

autor

Sardans J.

• CREAF, Cerdanyola del Valles, 08913 Catalonia, Spain
• CSIC, Global Ecology Unit CREAF-CEAB-CSIC-UAB, Cerdanyola del Vallès, 08913 Catalonia, Spain

autor

Perez-Trujillo M.

• Service of Nuclear Magnetic Resonance, Faculty of Sciences and Biosciences, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Catalonia, Spain

autor

Legido-Quigley C.

• Institute of Pharmaceutical Science, King’s College London, London SE1 9NH, England, U.K.

autor

Oravec M.

• Global Change Research Institute, Czech Academy of Sciences, Belidla 986/4a, 603 00 Brno, Czech Republic

autor

Urban O.

• Global Change Research Institute, Czech Academy of Sciences, Belidla 986/4a, 603 00 Brno, Czech Republic

autor

Elser J.J.

• School of Life Sciences, Arizona State University, Tempe, AZ 85287‑4501, USA

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Identyfikatory

DOI

10.1007/s11738-018-2603-1