Optimisation of the PCR for detection of Apxiva gene ofActinobacillus Pleuropneumoniae

• Autorzy: Urbaniak K. , Markowska-Daniel I.
• Języki publikacji: EN

Abstrakty

EN

PCR technique used for the detection of apxIVA gene of Actinobacillus pleuropneumoniae (App) was developed. The optimisation of the technique was conducted on App ATCC 27088 strain. The concentration of primers, Mg²⁺, and Taq polymerase, as well as the annealing temperature and the number of cycles were optimised. The test could detect up to 10⁻⁷ dilution, which equals 3x10¹ cfu/mL. The specificity of the PCR was verified with the use of genetic material of other pathogens existing in pigs' respiratory tract. Based on the obtained results it can be assumed that the developed PCR test can be used for the detection of the apxIVA gene, in both pure culture of App and lung tissue.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 2
• Opis fizyczny: p.181-185,fig.,ref.

Twórcy

autor

Urbaniak K.

• Department of Swine Disease, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Markowska-Daniel I.

Bibliografia

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