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2016 | 65 | 3 |
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Prevalence of biofilm formation and wide distribution of virulence associated genes among Vibrio spp. strains isolated from the Monastir Lagoon, Tunisia

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In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrioextracellular product were also observed among HeLa and Vero cells.
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  • Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia
  • Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia
  • Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia
  • Laboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, Tunisia
  • Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia
  • Akinbowale O.L., H. Peng and M.D. Barton. 2006. Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J. Appl. Microbiol. 100: 1103–1113.
  • Baffone W., E. Vittoria, R. Campana, B. Citterio, A. Casaroli and L. Pierfelice. 2005. Occurrence and expression of virulence related properties by environmental Vibrio spp. in vitro and in vivo systems. Food Control. 16: 451–457.
  • Balebona M.C., M.J. Andreu, M.A. Bordas, I. Zorrilla, M.A. Mori-n igo and J.J. Borrego. 1998. Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.). Appl. Environ. Microbiol. 64: 4269–4275.
  • Barbieri E., L. Falzano, C. Fiorentini, A. Pianetti, W. Baff one,A. Fabbri, P. Matarrese, A. Casiere, M. Katouli, I. Kuhn and others.1999. Occurrence, diversity, and pathogenicity of halophilic Vibrio spp. and non-O1 Vibrio cholerae from estuarine waters along the Italian adriatic coast. App. Envir. Microbio. 65: 2748–2753.
  • Barton C. and R. Acton. 2009. Hemochromatosis and Vibrio vul-nificus wound infections. J. Clin. Gastroenterol. 43(9): 890–893.
  • Bej A.K., D.P. Patterson, C.W. Brasher, M.C. Vickery, D.D. Jones and C.A. Kaysner. 1999. Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplexPCR amplification of tlh, tdh and trh. J. Microbiol. Methods. 36: 215–225.
  • Ben Kahla-Nakbi A., K. Chaieb, A. Besbes, T. Zmantar andA. Bakhrouf. 2006. Virulence and enterobacterial repetitive inter-genic consensus PCR of Vibrio alginolyticus strains isolated from Tunisian cultured gilthead sea bream and sea bass outbreaks. Ve t . Microbiol. 117: 321–327.
  • Ben Kahla-Nakbi A., A. Besbes, K. Chaieb, M. Rouabhiab and A. Bakhrouf. 2007. Survival of Vibrio alginolyticus in seawater and retention of virulence of its starved cells. Mar. Environ. Res.46: 469–478.
  • Cavallo J.D., H. Chardon, C. Chidiac, P. Choutet, P. Courvalin,H. Dabernat, H. Drugeon, L. Dubreuil, F. Goldstein, V. Jarlier and others. 2006. Antibiogram Committee of the French Microbiology Society. Report 2006 (in French)., 2014.10.01.
  • Cerca N., K.K. Jefferson, R. Oliveira, G.B. Pier and J. Azeredo.2006. Comparative antibody-mediated phagocytosis of Staphylococ-cus epidermidis cells grown in a biofilm or in the planktonic state. I.A.I. 74: 4849–4855.
  • Clinical and Laboratory Standards Institute (CLSI). 2006. Wayne, PA: Clinical and Laboratory Standards Institute, Methods for dilu-tion antimicrobial susceptibility tests for bacteria that grow aero-bically. M7-A7. 26: 14–16.
  • Colombo M.M., S. Mastrandea, A. Santona, A.P. De Amdrade,S. Uzzau, S. Rubino and P. Cappuccinelli. 1994. Distribution of the ace, zot and ctxA toxin genes in clinical and environmental Vibrio cholerae. J. Fish. Dis. 170: 750–751.
  • Costa R.A., G.C. Silva, J.R.O. Piexoto, G.H.F. Vieira and R.H.S.F. Vieira. 2010; Quantification and distribution of Vibrio species in water from an estuary in Ceará-Brazil impacted by shrimp farming. Braz. J. Oceanogr. 58(3): 183–188.
  • Costerton J.W., Z. Lewandowski, D.E. Caldwell, D.R. Korber and H.M. Lappin-Scott. 1995. Microbial biofilms. Ann. Rev. Microbiol.49: 711–745.
  • Fasano A., B. Baudry, D.W. Pumplin, S.S. Wasserman, B.D. Tall, J.M. Ketley and J.B. Kaper. 1991. Vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions. Proc. Natl. Acad. Sci. USA 88: 5242–5246.
  • Fields P.I., T. Popovic, K. Wachsmuth and O. Olsvik. 1992. Use of polymerase chain reaction for detection of toxigenic Vibrio chol e-rae O1 strains from the Latin American cholera epidemic. J. Clin. Microbiol. 30: 2118–2121.
  • Gomathi R.S., R. Vinothkumar and K. Arunagiri. 2013. Isolation and identification vibrios from marine seafood samples. Int. J. Curr. Microbiol. App. Sci. 2(2): 36–43.
  • Gomez-Leon J., L. Villamil, M.L. Lemos, B. Novoa and A. Figu-eras. 2005. Isolation of Vibrio alginolyticus and Vibrio splendidus from aquacultured carpet shell clam (Ruditapes decussatus) larva associated with mass mortalities. Appl. Environ. Microbiol. 71(1): 98–104.
  • Mohammadi-Barzelighi H., B. Bakhshi, A. Rastegar-Lariand and M.R. Pourshafie. 2011. Characterization of pathogenicity island prophage in clinical and environmental strains of Vibrio cholerae.J. Med. Microbiol. 60: 1742–1749.
  • Hall-Stoodley L., J.W. Costerton and P. Stoodley. 2004. Bacterial biofilms: from the natural environment to infectious diseases. Nat. Rev. Microbiol. 2: 95–108.
  • Henriques M., C. Sousa, M. Lira, M. Elisabete, R. Oliveira andJ. Azeredo. 2005. Adhesion of Pseudomonas aeruginosa and Staphy-lococcus epidermidis to silicone-hydrogel contact lenses. Optom. Vis. Sci. 82: 446–450.
  • Hiyoshi H., T. Kodama, T. Iida and T. Honda. 2010. Contribu-tion of Vibrio parahaemolyticus virulence factors to cytotoxicity, enterotoxicity, and lethality in mice. Infect. Immun. 78: 1772–1780.
  • Hoffmann F., O. Larsen, V. Thiel, H.T. Rapp, T. Pape, W. Michaelis and J. Reitner. 2005. An anaerobic world in sponges. Geomicro-biol.J. 22: 1–10.
  • Keasler S.P. and R.H. Hall. 1993. Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet 341: 1661.
  • Khouadja S., E. Suffredini, B. Baccouche, L. Croci and A. Bak-hrouf. 2014. Occurrence of virulence genes among Vibrio choleraeand Vibrio parahaemolyticus strains from treated wastewaters. Envi-ron. Monit. Assess. 186: 6935–6945
  • Li X.C., Z.Y. Xiang, X.M. Xu, W.H. Yan and J.M. Ma. 2009. Endophthalmitis caused by Vibrio alginolyticus. J. Clin. Microbiol.47(10): 3379–3381
  • Lin Z., K. Kumagai, K. Baba, J.J. Mekalanos and M. Nishibuchi.1993. Vibrio parahaemolyticus has a homolog of the Vibrio chole-rae toxRSoperon that mediates environmentally induced regula-tion of the thermostable direct hemolysin gene. J. Bacteriol. 175: 3844–3855.
  • Liu P.C., K.K. Lee and S.N. Chen. 1996. Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon. Lett. Appl. Microbiol. 22: 413–416.
  • Manjusha S., G.B. Sarita, K.K. Elyas and M. Chandrasekaran.2005. Multiple antibiotic resistances of Vibrio isolates from coastal and brackish water areas. A.J. Biochem. Biotechnol. 1: 201–206.
  • Matsumoto C., J. Okuda, M. Ishibashi, M. Iwanaga, P. Vi Garg, T. Rammamurthy, H. Wong, A. DePaola, Y.B. Kim, M.J. Albert and others. 2000. Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J. Clin. Micro-biol. 38(2): 578–585.
  • Mechri B., A. Medhioub, M.N. Medhioub and M. Aouni. 2012. Diversity of Vibrionaceae associated with Ruditapes decussatus hatchery in Tunisia. Ann. Microbiol. 61(2): 597–606.
  • Mechri B., R. Mabrouki-Charfeddine, A. Medhioub, M.N. Med-hioub and M. Aouni. 2013a. Adhesive properties and antibacterial susceptibility of Vibrio alginolyticus strains isolated from a Tunisian Ruditapes decussatus hatchery. A.J.M.R. 7(31): 4022–4030.
  • Mechri B., A. Medhioub, M.N. Medhioub and M. Aouni. 2013b. Genotypic Diversity, Antimicrobial resistance and screening of Vibrio cholerae molecular virulence markers in Vibrio alginolyticusstrains recovered from a Tunisian Ruditapes decussatus hatchery. Pol. J. Microbiol. 62(3): 263–272.
  • Miller J.F., J.J. Mekalanos and S. Falkow. 1989. Coordinate regula-tion and sensory transduction in the control of bacterial virulence. Science 243: 916–922.
  • Miller V.L., R.K. Taylor and J.J. Mekalanos. 1987. Cholera toxin transcriptional activator toxR is a trans-membrane DNA binding protein. Cell 48: 271–279.
  • Morris A.R. and K.L. Visick. 2010. Control of biofilm formation and colonization in Vibrio fischeri: a role for partner switching? Environ. Microbiol. 12: 2051–2059.
  • Nair G.B. and J.C. Hormazabal. 2005. The Vibrio parahaemolyticus pandemic. Rev. Chil. Infectol. 22: 125–130.
  • Nair G.B., T. Ramamurthy, S.K. Bhattacharya, B. Dutta, Y. Takeda and D.A.Sack. 2007. Global dissemination of Vibrio parahaemo-lyticus serotype O3:K6 and its serovariants. Clin. Microbiol. Rev. 20: 39–48.
  • Nandi, B., R. K. Nandy, S. Mukhopadhyay, G. B. Nair, T. Shimada, and A.C. Ghose. 2000. Rapid method for species-specific identifica-tion of Vibrio cholerae using primers targeted to the gene of outer membrane protein ompW. J. Clin. Microbiol. 38: 4145–4151.
  • National Committee for Clinical Laboratory Standards (NCCLS).2002 Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Tentative standards. Document M31-T. NCCLS. Wayne.
  • Nhung P.H., M.M. Shah, K. Ohkusu, M. Noda, H. Hata, X.S. Sun, H. Iihara, K. Goto, T. Masaki, J. Miyasaka and T. Ezaki. 2007. DnaJ gene as a novel phylogenetic marker for identification of Vibrio species. J. Syst. Appl. Microbiol. 30: 309–315.
  • Ottaviani D., I. Bacchiocchi, L. Masini, F. Leoni, A. Carraturo,M. Giammarioli and G. Sbaraglia. 2001. Antimicrobial suscepti-bility of potentially pathogenic halophilic vibrios isolated from sea food. Int. J. Antimicrobial. Agents. 18: 135–140.
  • Peterson K.M. 2002. Expression of Vibrio cholerae virulence genes in response to environmental signals. Curr. Issues. Intest. Microbiol.3: 29–38.
  • Reboucas R.H., O.A. de Sousa, A.S. Lima, F.R. Vasconcelos,P.B. de Carvalho and R.H.S.F. Vieira. 2011. Antimicrobial resist-ance profile of Vibrio species isolated from marine shrimp farming environments (Litopenaeus vannamei) at Ceará, Brazil. Environ Res.111(1): 21–24.
  • Reilly G.D., C.A. Reilly, E.G. Smith and C. Baker-Austin. 2011. Vibrio alginolyticus associated wound infection acquired in British water. Eurosurveillance. 16: 1–2.
  • Ren C., C. Hu, X. Jiang, H. Sun, Z. Zhao, C. Chen and P. Luo.2013. Distribution and pathogenic relationship of virulence associa-ted genes among Vibrio alginolyticus from the mariculture sys-tems. Mol. Cell Probe. 27:164–168.
  • Rivera I.N.G., J. Chun, A. Huq, R.B. Sack and R.R. Colwell. 2001. Genotypes associated with virulence in environmental isolates of Vibrio cholera. Appl. Environ. Microbiol. 67: 2421–2429.
  • Scoglio M.E., A. Di Pietro, I. Picerno, S. Delia, A. Mauro andP. Lagana. 2001. Virulence factors in Vibrios and Aeromonads iso-lated from seafood. New. Microbiol. 24: 273–280.
  • Sechi L.A., I. Dupre, A. Deriu, G. Fadda and S. Zanetti. 2000. Dis-tribution of Vibrio cholerae virulence genes among different Vibriospecies isolated in Sardinia., Italy. J. Appl. Microbiol. 88: 475–481.
  • Snoussi M., K. Chaieb, M. Rouabhia and A. Bakhrouf. 2006. Quantitative study, identification and antibiotics sensitivity of some Vibrionaceae associated to a marine fish hatchery. Ann. Microbiol.56(4): 289–293.
  • Snoussi M., E. Noumi, D. Usai, L.A. Sechi, S. Zanetti andA. Bakhrouf. 2008. Distribution of some virulence related-proper-ties of Vibrio alginolyticus strains isolated from Mediterranean sea-water (Bay of Khenis, Tunisia): investigation of eight Vibrio chole raevirulence genes. World. J. Microbiol. Biotech. 24: 2133–2141.
  • Sonia A.S. and A.P. Lipton. 2012. Pathogenicity and antibiotic sus-ceptibility of Vibrio species isolated from the captive-reared tropical marine ornamental blue damsel fish, Pomacentrus caeruleus (Quoy and Gaimard, 1825). Indian J. Geo-Marine Sci. 41: 348–354.
  • Stepanovic S., D. Vukovic, I. Dakic, B. Savic and M. Švabić-Vlahovic. 2000. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J. Microbiol. Methods. 40: 175–179.
  • Trucksis M., J.E. Galen, J. Michalski, A. Fasano, and J.B. Kaper.1993. Accessory cholera enterotoxin (ace), the third toxin of a V. cholerae virulence cassette. PNAS 90: 5267–5271.
  • Vaseeharan B., P. Ramasamy, T. Murugan and J.C. Chen. 2005. In vitro susceptibility of antibiotics against Vibrio spp. and Aero-monas spp. isolated from Penaeus monodon hatcheries and ponds. Int. J. Antimicrob. Agents. 26: 285–291.
  • Xie Z.Y., C.Q. Hu, C. Chen, L.P. Zhang and C.H. Ren. 2005. Inves-tigation of seven Vibrio virulence genes among Vibrio algi nolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong. China. Lett. Appl. Microbiol. 41: 202–207.
  • Yoder J., M. Hlavsa, G. Craun, V. Hill, V. Roberts, P. Yu, L. Hicks, N. Alexander, R. Calderon, S. Roy and others. 2008. Surveillance for waterborne disease and outbreaks associated with recreational water use and other aquatic facility-associated health events – united states, 2005–2006. MMWR Surveill Summ. 57(9): 1–29.
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