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2012 | 56 | 1 |
Tytuł artykułu

Molecular detection of Bartonella sp. in wild ruminants and analysis of its genetic diversity on the basis of 168-23S rRNA intergenic spacer (ITS)

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to describe the state of infection of roe deer (Capreolus capreolus) and red deer (Cervus elaphus) by Bartonella sp. in North-Western Poland through PCR detection of Bartonella 16S-23S rRNA ITS region in isolates of animal tissues, and also to describe the genetic diversity of detected Bartonella species based on molecular analysis of ITS. The multiple alignment analysis of ITS sequences was carried out, and homology matrices and phylogenetic trees were constructed. The DNA of Bartonella sp. was detected in tissues of 45.6% (36/79) C. capreolus and of 50% (15/30) C. elaphus. Products of two different sizes were detected: 317 bp, characteristic for B. schoenbuchensis, and 198 bp, characteristic for B. bovis. The obtained results suggest that roe and red deer are potential reservoirs of Bartonella sp. Most of the analysed ITS sequences was not specific for one host species. In constructed phylogenetic trees, sequences obtained from roe and red deer clustered together. These results suggest a lack of host specificity of most detected B. schoenbuchensis and B. bovis intraspecies strains.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
56
Numer
1
Opis fizyczny
15-19,fig.,ref.
Twórcy
autor
  • Department of Genetics, Faculty of Biology, University of Szczecin, 71-412 Szczecin, Poland
Bibliografia
  • 1. Adamska M.: Wild ruminants in the area of the north-western Poland as potential reservoir hosts of Bartonella schoenbuchensis and Bartonella bovis. Acta Parasitol 2008, 53, 407-410.
  • 2. Bai Y., Cross P.C., Malania L., Kosoy M: Isolation of Bartonella capreoli from elk. Vet Microbiol 2011, 148, 329-332.
  • 3. Bermond D., Boulouis H.J., Heller R., Van Laere G., Monteil H., Chomel B.B., Sander A., Dehio C., Piemont Y.: Bartonella bovis Bermond et al. sp. nov. and Bartonella capreoli sp. nov., isolated from European ruminants. Int J Syst Evol Microbiol 2002, 52, 383-390.
  • 4. Boulouis H.J., Chang C.-C., Henn J.B., Kasten R.W., Chomel B.B.: Factors associated with the rapid emergence of zoonotic Bartonella infections. Vet Res 2005, 36, 383-410.
  • 5. Chang C.-C., Chomel B.B., Kasten R.W., Heller R., Kocan K.M., Ueno H., Yamamoto K., Bleich V.C., Pierce B.M., Gonzales B.J., Swift P.K., Boyce W.M., Jang S.S., Boulouis H.-J., Piemont Y.: Bartonella spp. isolated from wild and domestic ruminants in North America. Emerg Infect Dis 2000, 6, 306-311.
  • 6. Dehio C., Lanz C., Pohl R., Behrens P., Bermond D., Piemont Y., Sander A.: Bartonella schoenbuchii sp. nov., isolated from the blood of deer. Int J Syst Evol Microbiol 2001, 51, 1557-1565.
  • 7. Houpikian P., Raoult D.: 16S/23S rRNA intergenic spacer regions for phylogenetic analysis, identification, and subtyping of Bartonella species. J Clin Microbiol 2001, 39, 2768-2778.
  • 8. Inoue K., Kabeya H., Hagiya K., Kosoy M.Y., Une Y., Yoshikawa Y., Maruyama S.: Multi-locus sequence analysis reveals host specific association between Bartonella washoensis and squirrels. Vet Microbiol 2011, 148, 60-65.
  • 9. Jacomo B., Kelly P.J., Raoult D.: Natural history of Bartonella infections (an exception to Koch's postulate). Clin Diagn Lab Immunol 2002, 9, 8-18.
  • 10. Jensen W., Fall M.Z., Rooney J., Kordick D.L., Breitschwerdt A.B.: Rapid identification and differentiation of Bartonella species using a single-step PCR assa>. J Clin Microbiol 2000, 38, 1717-1722.
  • 11. Kimura M.: A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980, 16, 111-120.
  • 12. Maillard R., Vayssier-Taussat M., Bouillin C., Gandoin C., Halos L., Chomel B., Piemont Y., Boulouis H.J.: Identification of Bartonella strains isolated from wild and domestic ruminants by a single-step PCR analysis of the 16S-23S intergenic spacer region. Vet Microbiol 2004, 1, 63-69.
  • 13. Maillard R., Grimard B., Chastant-Maillard S., Chomel B., Delcroix T., Gantoin C., Bouillin C., Halos L., Vayssier-Taussat M., Boulouis H.-J.: Effects of cow age and pregnancy on Bartonella infection in a herd of dairy cattle. J Clin Microbiol 2006, 44, 42-46.
  • 14. Maggi R.G., Breitschwerdt E.B.: Potential limitations of the 16S-23S rRNA intergenic region for molecular detection of Bartonella species. J Clin Microbiol 2005, 43, 1171-1176.
  • 15. Rolain J.M., Rousset E., La Scola B., Duquesnel R., Raoult D.: Bartonella schoenbuchensis isolated from the blood of a French cow. Ann N Y Acad Sei 2003, 990, 236-238.
  • 16. Skotarczak B., Adamska M.: Capreolus capreolus and Ixodes ricinus as a reservoir of Bartonella in northwestern Poland. Wiad Parazytol 2005, 51, 139-143.
  • 17. Stojek N., Zwoliński J.: Cat scratch disease and other diseases caused by bacteria of Bartonella genus. Medycyna Wet 2003, 59, 660-664.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-096df438-5fdd-41d0-8854-2595463998e1
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