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2013 | 57 | 3 |
Tytuł artykułu

Analysis of proapoptotic changes in boar spermatozoa stored at 16 C in different short-term semen extenders

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to evaluate the effect of boar semen storage in different short-term extenders (BTS, Kortowo-3, and M III) on the percentage of spermatozoa showing proapoptotic and necrotic changes. For the first time in this study, Annexin V isolated from swine placenta has been used to determine proapoptotic changes in stored boar spermatozoa. Die changes were determined using the IN Cell Analyzer 2000. A gradual decrease in motility was observed on successive days of storage. Spermatozoa incubated in the BTS extender were characterised by the highest average motility, which reached 75% on the 1st d and 39% on day 5. Motility of spermatozoa stored in BTS was significantly higher than those stored in Kortowo-3 and M III extenders after 5 d of storage. Diluted semen contained 1.5% to 2.8% spermatozoa with proapoptotic changes. The discussed process was intensified on the 3rd d of storage when the percentage of apoptotic spermatozoa was determined at 8.3% to 14.6%, and the content of dead spermatozoa exceeded 25%. The analysed extenders differed insignificantly in their ability to protect semen against proapoptotic changes during storage. From the methodological point of view, Hoechst 33258 could be used additionally to stain sperm cells regardless of their status.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
57
Numer
3
Opis fizyczny
p.425-428,ref.
Twórcy
autor
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland
Bibliografia
  • 1. Anzar M., He L., Buhr M.M., Kroetsch T.G., Pauls K.P.: Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility. Biol Reprod 2002, 66, 354-360.
  • 2. Fraser L., Lecewicz M., Strzeżek J.: Fluorometric assessments of viability and mitochondrial status of boar spermatozoa following liquid storage. Pol J Vet Sci 2002, 5, 85-92.
  • 3. Fraser L., Pareek C.S., Strzeżek J.: Identification of amplified fragment length polymorphism markers associated with freezability of boar semen a preliminary study. Med Weter 2008, 64, 646-649.
  • 4. Gadella B., Harrison R.: Capacitation induces cyclic adenosine 3', 5' - monophosphate dependent, but apoptosis - unrelated, exposure of sperm cells. Biol Reprod 2002, 67, 340-350.
  • 5. Huo L.J., Ma X.H., Yang Z.M.: Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology 2002, 58, 1349-1360.
  • 6. Ivanova-Kicheva M., Strzeżek J., Kukov A., Wysocki P., Solenkova E., Daskalova D., Todorov L.: Isolation of Annexin V from swine placenta to assay cell plasma membrane phosphatidylserine translocation. C R Acad Bulg Sci 2008, 6, 753-760.
  • 7. Johnson I.A., Weitze K.F., Fiser P., Maxwell W.M.C.: Storage of boar semen. Anim Reprod Sci 2000, 62, 143-172.
  • 8. Kumaresan A., Kadirvel G., Bujarbaruah K.M., Bardoloi R.K., Das A., Kumar S., Naskar S.: Preservation of boar semen at 18°C induces lipid peroxidation and apoptosis like changes in spermatozoa. Anim Reprod Sci 2009, 110, 162-171.
  • 9. Martin G., Sabido O., Durand P., Levy R.: Cryopreservation induces an apoptosis like mechanism in bull sperm. Biol Reprod 2004, 71, 28-37.
  • 10. Pena F.J., Johannisson A., Wallgrena M., Rodrıguez Martınez H.: Assessment of fresh and frozen-thawed boar semen using an Annexin-V assay: a new method of evaluating sperm membrane integrity. Theriogenology 2003, 60, 677-689.
  • 11. Sakkas D., Moffatt O., Manicardi G.C., Mariethoz E., Tarozzi N., Bizzaro D.: Nature of DNA damage in ejaculated spermatozoa and the possible involvement of apoptosis. Biol Reprod 2002, 66, 1061-1067.
  • 12. Shen H.M., Ong C.: Detection of oxidative DNA damage in human sperm and its association with sperm function and male fertility. Free Radie Biol Med 2000, 28, 529-536.
  • 13. Strzeżek J., Strzeżek R., Dziekońska A., Fraser L.: Dialysis of boar semen prior to freezing-thawing: Its effects on post-thaw sperm characteristics. Theriogenology 2007, 67, 994-1003.
  • 14. Trzcińska M., Bryła M., Smorąg Z.: 2006. Correlation between two apoptotic markers in fresh boar spermatozoa. Reprod Fert Dev 2006, 19, 280-281.
  • 15. Trzcińska M., Bryła M., Smorąg Z.: Apoptotic-like changes in the spermatozoa of fresh and stored boar semen and the quality of embryos produced in vivo. Anim Reprod Sci 2011, 124, 90- 97.
  • 16. Weng S.L., Taylor S.L., Morshedi M., Schuffner A., Duran E.H., Beebe S., Oehninger S.: Caspase activity and apoptotic markers in ejaculated human sperm. Mol Hum Reprod 2002, 8, 984-991.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-09351c56-95bb-493f-9904-21a0f6e4a162
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