Natural hybrid zone of Aconitum species in the Western Carpathians: Linnaean taxonomy and ISSR fingerprinting

• Autorzy: Sutkowska A. , Boron P. , Mitka J.
• Języki publikacji: EN

Abstrakty

EN

Aconitum lasiocarpum (Carpathian endemic) and A. variegatum (European endemic) occur sympatrically in the Polish Western Carpathians. Here their taxonomic hybrid A. ×pawlowskii occurs. The aim of this study was to determine the relationship between the taxonomic (Linnaean approach) and genetic structure (PCR-ISSR analysis) of the populations and individuals in two allopatric and four sympatric populations. We determined 309 individuals (OTUs) to species, subspecies and nothospecies using the Linnaean system of classification, and then genetically fingerprinted 39 randomly chosen OTUs. Comparison of the Nei and Li distances obtained from ISSR and morphological matrices using the Mantel test indicated a significant correlation (n = 39, r = 0.53, P = 0.001). Genetic analysis (NEWHYBRIDS) pointed to 7 OTUs as being later-generation hybrids (B1 introgessants) in the sympatric area. Five of them belong to A. variegatum, indicating cryptic introgression, and two belong to A. ×pawlowskii. Nonmetric multidimensional scaling (NDMS) showed gene flow between A. lasiocarpum and A. ×pawlowskii. Allopatric, morphologically pure A. lasiocarpum and A. variegatum populations differed significantly in their ISSR profiles (Fischer's R×C test, P < 0.0001). Expected heterozygosity (Hj) was significantly (p=0.05) lower in allopatric (0.1261–0.1268) than in sympatric populations (0.1348–0.1509), indicating a genetic melting pot in sympatry. The results support the existence of a natural interspecific hybrid swarm zone in the sympatric area of occurrence of Aconitum, and the taxonomic circumscription of the nothospecies within the Linnaean taxonomic system.

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2013
• Tom: 55
• Numer: 1
• Opis fizyczny: p.114-126,fig.,ref.

Twórcy

autor

Sutkowska A.

• Department of Plant Breeding and Seed Science, University of Agriculture, Łobzowska 24, 31-140 Cracow, Poland

autor

Boron P.

• Department of Forest Phytopathology, University of Agriculture, Al. 29 Listopada 46, 31-425 Cracow, Poland

autor

Mitka J.

• Botanical Garden, Jagiellonian University, Kopernika 27, 31-501 Cracow, Poland

Bibliografia

• ANDERSON EC, and THOMPSON EA. 2002. A model-based method for identifying species hybrids using multilocusgenetic data. Genetics 160: 1217–1229.
• APOSTOL BL, BLACK IV WC, REITER P, and MILLER BR. 1996. Population genetics with RAPD-PCR markers: the breedingstructure of Aedes aegypti in Puerto Rico. Heredity76: 325–334.
• ARNOLD ML. 1992. Natural hybridization as an evolutionary process. Annual Review of Ecology and Systematics 23:237–261.
• ARNOLD ML. 1997. Natural Hybridization and Evolution. Oxford University Press, New York, Oxford.
• ARNOLD ML, TANG S, KNAPP SJ, and MARTIN NH. 2010. Asymmetric introgressive hybridization amongLouisiana iris species. Genes 1: 9–22.
• BARTON NH, and CHARLESWORTH B. 1984. Genetic revolutions, founder effects, and speciation. Annual Review ofEcology and Systematics 15: 133–164.
• BARTON NH, and HEWITT GM. 1985. Analysis of hybrid zones. Annual Review of Ecology and Systematics 16:113–148.
• BONIN A, BELLEMAIN E, BRONKEN EIDESEN P, POMPANON F, BROCHMANN C, and TABERLET P. 2004. How to track andassess genotyping errors in population genetics studies. Molecular Ecology 13(11): 3261–73.
• BONIN A, EHRICH D, and MANEL S. 2007. Statistical analysis of amplified fragment length polymorphism data: a toolboxfor molecular ecologists and evolutionists. MolecularEcology 16: 3737–3758.
• BREITENBACH-DORFER M, KONNERT M, PINSKER W, STARLINGER F, and GEBUREK T. 1996. The contact zone between twomigration routes of silver fir, Abies alba (Pinaceae),revealed by allozyme studies. Plant Systematics andEvolution 206: 259–272.
• BUSSELL JD, WAYCOTT M, and CHAPPILL JA. 2005. Arbitrarily amplified DNA markers as characters for phylogenetic inference. Perspectives in Plant Ecology, Evolution and Systematics 7(1): 3–26.
• CHUNG MG, and PARK CW. 2000. Notes on spatial genetic structure in a hybrid population between Aconitum japonicum subsp. napiforme and A. jaluense(Ranunculaceae). Annales Botanici Fennici 37:243–247.
• DHAR U, and SAMANT S. 1993. Endemic plant diversity in the Indian Himalaya. I. Ranunculaceae to Paeoniaceae. Journal of Biogeography 20(6): 659–668.
• FISHER RA. 1954. Statistical methods for research workers. 12th ed. Oliver and Boyd, Edinburgh. GAYER G. 1909. Vorarbeiten zu einer Monographie der europaischen Aconitum-Arten. Magyar Botanikai Lapok5: 122–137.
• GONZALES-RODRIGUEZ A, ARIAS DM, VALENCIA S, and OYAMA K. 2004. Morphological and RAPD analysis of hybridization between Quercus affinis and Q. laurina (Fagaceae), two Mexican red oaks. American Journal of Botany 91(3):401–409.
• GOTZ E. 1967. Die Aconitum variegatum-Gruppe und ihre Bastarde in Europa. Feddes Repertorium 76: 1–62.
• HUBISZ MJ, FALUSH D, STEPHENS M, and PRITCHARD JK. 2009. Inferring weak population structure with the assistanceof sample group information. Molecular EcologyResources 9(5): 1322–1332.
• HUSBAND BC. 2004. The role of triploid hybrids in the evolutionary dynamics of mixed-ploidy populations.Biological Journal of the Linnean Society 82(4):537–546.
• HUSON DH, and BRYANT D. 2006. Application of Phylogenetic Networks in Evolutionary Studies. Molecular Biologyand Evolution 23(2): 254–267.
• ILNICKI T, JOACHIMIAK A, SUTKOWSKA A, and MITKA J. 2011. Cytotypes distribution of Aconitum variegatum L. inCentral Europe. In: Zemanek B [ed.], Geobotanist andTaxonomist. A Volume Dedicated to Professor A. Zającon the 70th Anniversary of His Birth, 169–192. Instituteof Botany, Jagiellonian University, Cracow.
• ILNICKI T, and MITKA J. 2009. Chromosome numbers in Aconitum sect. Aconitum (Ranunculaceae) from theCarpathians. Caryologia 62(3): 198–203.
• ILNICKI T, and MITKA J. 2011. Chromosome numbers in Aconitum sect. Cammarum (Ranunculaceae) from theCarpathians. Caryologia 64(4): 446–452.
• JOACHIMIAK A, ILNICKI T, and MITKA J. 1999. Karyological study on Aconitum lasiocarpum (Rchb.) Gayer(Ranunculacea). Acta Biologica Cracoviensia SeriesBotanica 41: 205–211.
• KADOTA Y. 1981. A taxonomic study of Aconitum (Ranunculaceae) of the Akaishi Mountain in CentralJapan. Bulletin of the National Science Museum. SeriesB, Botany 7(3): 91–114.
• KADOTA Y. 1986. Aconitum of the Ryohaku Mountain Range, Central Japan – a new subspecies of A. zigzag Lev. etVant. and the entity of "A. hakusanense" Nakai. Memorisof the National Science Museum, Tokyo 19: 133–144.
• KADOTA Y. 1987. A Revision of Aconitum Subgenus Aconitum (Ranunculaceae) of East Asia. Sanwa Shoyaku Company,Ltd., Utsunomiya.
• KONNERT M, and BERGMANN F. 1995. The geographical distribution of genetic variation of silver fir (Abies alba,Pinaceae) in relation to its migration history. PlantSystematics and Evolution 196: 19–30.
• LIHOVA J, KOCHJAROVA J, and MARHOLD K. 2007. Hybridization between polyploids Cardamine enneaphyllos and C.glandulifera in the West Carpathians: evidence frommorphology, pollen fertility and PCR-RFLP pattern.Preslia 79: 101–125.
• LIM CE, PARK J-K, and PARK C-W. 1999. Flavonoid variation of the Aconitum jaluense complex (Ranunculaceae) in Korea. Plant Systematics and Evolution 218: 125–131.
• LUFEROV AN. 2000. Konspekt kavkazkih vidov Aconitum (Ranunculaceae). Botanieeskij Zhurnal 85(10): 87–96. (In Russian).
• LUO Y, ZHANG F, and YANG Q. 2005. Phylogeny of Aconitum subgenus Aconitum (Ranunculaceae) inferred ITS sequences. Plant Systematics and Evolution 252:11–25.
• LYNCH M, and MILLIGAN B. 1994. Analysis of population-genetic structure using RAPD markers. Molecular Ecology 3:91–99.
• MANLY BFJ. 1991. Randomization and Monte Carlo Methods in Biology. Chapman and Hall, NY.
• MANTEL N. 1967. Detection of disease, clustering and generalized regression approach. Cancer Resources 27: 209–220.
• MILLER MP. 1997. Tools for Population Genetic Analyses (TFPGA) version 1.3 A WindowsR program for the analysisof allozyme and molecular population genetic data.Department of Biological Sciences, Northern ArizonaUniversity, Flagstaff.
• MILNE RI, and ABBOTT RJ. 2008. Reproductive isolation among two interfertile Rhododendron species: low frequency of post-F1 hybrid genotypes in alpine hybrid zones. Molecular Ecology 17: 1108–1121.
• MINDER AM, ROTHENBUEHLER C, and WIDMER A. 2007. Genetic structure of hybrid zones between Silene latifolia andSilene dioica (Caryophyllaceae): evidence for introgressivehybridization. Molecular Ecology 16: 2504–2516.
• MITKA J. 2003. The genus Aconitum L. (Ranunculaceae) in Poland and Adjacent Countries. A Phenetic-GeographicStudy. Institute of Botany, Jagiellonian University,Krakow.
• MITKA J, SUTKOWSKA A, ILNICKI T, and JOACHIMIAK AJ. 2007. Reticulate evolution of high-alpine Aconitum(Ranunculaceae) in the Eastern Sudetes and WesternCarpathians. Acta Biologica Cracoviensia, seriesBotanica 49(2): 15–26.
• MITKA J, and STARMUHLER W. 2000. Phenetic variability of Aconitum lasiocarpum (Rchb.) Gayer (Ranunculaceae): extension of taxonomic and geographic borders. Acta Societatis Botanicorum Poloniae 69(2): 145–155.
• MRAZ P, CHRTEK J, FEHRER J, and PLAEKOVA I. 2005. Rare recent natural hybridization in Hieracium s. str. – evidence from morphology, allozymes and chloroplast DNA. Plant Systematics and Evolution 255: 177–192.
• MRAZ P, GAUDEUL M, RIOUX D, GIELLY L, CHOLER P, TABERLET P, and THE INTRABIODIV CONSORTIUM. 2007. Geneticstructure of Hypochoreris uniflora (Asteraceae) suggestsvicariance in the Carpathians and rapid post-glacial colonizationon the Alps from an eastern Alpine refugium. Journal of Biogeography 34: 2100–2114.
• MRAZ P, and SZELąG Z. 2004. Chromosome numbers and reproductive systems in selected species of the generaHieracium L. and Pilosella Hill (Asteraceae) fromRomania. Annales Botanici Fennici 41: 405–414.
• MUCHER W. 1991. Der Bunte Eisenhut, Aconitum variegatum L. (Ranunculaceae), in der Steiermark. Mitteilungen des Naturwissenschaftlichen Vereines fur Steiermark 121: 195–198.
• NEI M. 1972. Genetic distance between populations. American Naturalist 106: 283–292.
• NEI M, and LI WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences USA76: 5269–5273.
• NKONGOLO KK, DEVERNO L, and MICHAEL P. 2003. Genetic validation and characterisation of RAPD markers differentiatingblack and red spruces: molecular certification ofspruce trees and hybrids. Plant Systematics andEvolution 236: 151–163.
• OH S, and PARK C-W. 1998. Crossability of the Aconitum jaluense species complex (Ranunculaceae) in Korea. Korean Journal of Biological Sciences 2: 435–438.
• PARK C-W, OH S, LIM CE, and SUH Y. 1997. Morphological evidence of hybridization between Aconitum japonicumsubsp. napiforme and A. jaluense subsp. jaluense(Ranunculaceae). Journal of Japanese Botany 72:144–153.
• PETIT R, AGUINAGALDE I, BEAULIEU J, BITTKAU C, BREWER S, CHEDDADI R, ENNOS R, FINESCHI S, GRIVET D, LASCOUX M, MOHANTY A, MULLER-STARK G, DEMESURE-MUSCH B, PALMEE A, MARTIIN J, RENDELL S, VENDRAMIN G. 2003. Glacial refugia: hotspots but not melting pots of geneticdiversity. Science 300: 1563–1565
• PRITCHARD JK, STEPHENS M, and DONELLY P. 2000. Inference of population structure using multilocus genotype data.Genetics 155: 945–959.
• PROVAN J, and BENNETT KD. 2008. Phylogeographic insights into cryptic glacial refugia. Trends in Ecology andEvolution 23: 564–571.
• RAYMOND ML, and ROUSSET F.1995. An exact test for population differentiation. Evolution 49: 1280–1283.
• REYNOLDS J, WEIR BS, and COCKERHAM CC. 1983. Estimation of the coancestry coefficient: basis for a short-term geneticdistance. Genetics 105: 767–779.
• RIESEBERG LH, BAIRD SJE, and GARDNER KA. 2000. Hybridization, introgression, and linkage evolution.Plant Molecular Biology 42: 205–224.
• ROHLF FJ. 2002. NTSYS-pc. Numerical taxonomy and multivariate analysis, version 2.1. Exeter Software, Setauket,New York, USA.
• RONIKIER M. 2011. Biogeography of high-mountain plants in the Carpathians: An emerging phylogeographical perspective.Taxon 60: 373–389.
• RUSHAM M, HOLLINGSWORTH PM, ENNOS RA. 2011. Early evolution in a hybrid swarm between outcrossing and selfinglineages in Geum. Heredity 107(3): 246–255.
• SALE M, POTTS BM, and WEST AK. 1996. Molecular differentiation within and between Eucalyptus ridsonii and E.amygdalina and their hybrids using RAPD markers. Australian Journal of Botany 44: 559–569.
• SEITZ W. 1969. Die Taxonomie der Aconitum napellus- Gruppe in Europa. Feddes Repertorium 80(1): 1–76.
• SICA M, GAMBA G, MONTIERI S, GAUDIO L, and ACETO S. 2005. ISSR markers show differentiation among Italian populationsof Asparagus acutifolius L. BMC Genetics 6: 17.
• SOKAL RR, and MICHENER CD. 1958. A statistical method for evaluating systematic relationships. University ofKansas Scientific Bulletin 38: 409–438.
• SOKAL R, and ROHLF FJ. 1995. Biometry. 3rd edition. W.H. Freeman and Co., New York.
• STACHURSKA-SWAKOŃ A, CIEŚLAK E, and RONIKIER M. 2011. Phylogeography of subalpine tall-herb species in CentralEurope: the case of Cicerbita alpina (L.) Wallr. Preslia84(1): 121–140.
• STEPANSKY A, KOVALSKI I, and PERL-TREVES R. 1999. Interspecific classification of melons (Cucumis melo L.)in view of their phenotypic and molecular variation.Plant Systematics and Evolution 271: 313–332.
• UTELLI AB, ROY BA, and BALTISBERGER M. 1999. History can be more important than 'pollination syndrome' in determiningthe genetic structure of plant populations: the case ofAconitum lycoctonum (Ranunculaceae). Heredity 82:574–584.
• VEKEMANS X. 2002. AFLP-SURV version 1.0. Distributed by the author. Laboratoire de Genetique et Ecologie Vegetale, Universite Libre de Bruxelles, Belgium.
• WOLFE AD, XIANG QY, and KEPHART SR. 1998. Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable intersimplesequence repeat (ISSR) bands. Molecular Ecology 7:1107–1125.
• ZAJĄC M. 1996. Mountain vascular plants in the Polish lowlands. Polish Botanical Studies 11: 1–92.
• ZAJĄC M, and ZAJĄC A. 2009. The Geographical Elements of Native Flora of Poland. Laboratory of ComputerChorology, Institute of Botany, Jagiellonian University,Cracow.
• ZEMANEK B. 1999. The phytogeographical boundry between the East and West Carpathians – past and present.Thaiszia – Journal of Botany (Košice) 1: 59–67.
• ZHIVOTOVSKY LA. 1999. Estimating population structure in diploids with multilocus dominant DNA markers.Molecular Ecology 8: 907–913.
• ZIELIŃSKI R. 1982a. An electrophoretic and cytological study of hybridisation between Aconitum napellus ssp.skerisoare (2n=32) and A. variegatum (2n=16). I Electrophoretic evidence. Acta Societatis BotanicorumPoloniae 51: 453–464.
• ZIELIŃSKI R. 1982b. An electrophoretic and cytological study of hybridisation between Aconitum napellus ssp.skerisoare (2n=32) and A. variegatum (2n=16). IICytological evidence. Acta Societatis BotanicorumPoloniae 51: 465–471.

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