Wpływ immunostymulacji karpi LPS Aeromonas hydrophila na odporność nieswoistą i wrażliwość na zakażenie w teście challenge

• Autorzy: Grochola A. , Sopinska A. , Puk K.

Warianty tytułu

EN

Effect of LPS from Aeromonas hydrophila on non-specific immune parameters and survival of carp (Cyprinus carpio L.) infected with Aeromonas hydrophila

• Języki publikacji: PL

Abstrakty

EN

Bacterial infections are a serious problem for commercial farms of carp (Cyprinus carpio L.) in Poland. Given the absence of efficient vaccines, it is crucial to search for new agents enhancing the non-specific immune response of the fish. In the present study, immunomodulating effects of lipopolysaccharide extracted from the virulent (LPS-P) and non-virulent (LPS-NP) strains of Aeromonas hydrophila were studied. For that purpose, different concentrations of LPS-P and LPS-NP (25, 50, and 75 µg/100 g body weight) were administered to test animals through intraperitoneal injection. The non-specific immune response of the fish were studied at days 7, 14, 21, 28, and 60 after vaccination. Immunostimulated fish showed an increase in total leukocyte count, in the population of monocytes and neutrophils, and in immature cell count in the whole blood. Phagocytic activity and the production of reactive oxygen species were also elevated after vaccination. Moreover, vaccinated fish showed a significant increase in serum lysozyme. The results of the present study show that LPS-NP has a greater immunostimulatory effect than LPS-P at doses of 50 and 75 µg/100 g body weight. In addition, 7 days after vaccination with LPS-NP (50 µg/100 g body weight), fish were challenged with the virulent strain of A. hydrophila. The relative percentage of survival in groups 1 and 2 was 58.82% and 76. 47%, respectively, which indicates that the administration of LPS-NP makes C. carpio more resistant to infection by A. hydrophila

Słowa kluczowe

PL

ryby; karpie; immunostymulacja; lipopolisacharydy; LPS Aeromonas hydrophila; odpornosc nieswoista; Aeromonas hydrophila; wrazliwosc na zakazenie; test challenge

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2015
• Tom: 71
• Numer: 03
• Opis fizyczny: s.176-181,rys.,bibliogr.

Twórcy

autor

Grochola A.

• Zakład Chorób Ryb i Biologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

autor

Sopinska A.

• Zakład Chorób Ryb i Biologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

autor

Puk K.

• Zakład Chorób Ryb i Biologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

Bibliografia

• 1. Avtalion R. R., Shahrabani R.: Studies on phagocytosis in fish. I. In vitro uptake and killing of living Staphylococcus aureus by peripheral leucocytesof carp (Cyprinus carpio). Immunol. 1975, 29, 1181-1187.
• 2. Avtalion R. R., Wojdani A., Malik Z., Shahrabani R., Ducziminer M.: Influence of environmental temperature on the immune response in fish. Curr. Top.Microbiol. Immunol. 1973, 61, 1-35.
• 3. Baba T., Imamura J., Izawa K.: Immune protection in carp, Cyprinus carpio L., after immunization with Aeromonas hydrophila crude lipopolysaccharide.J. Fish Dis. 1988, 11, 237-244.
• 4. Boivin A., Mesrobeanu I., Mesrobeanu L.: Technique pour la preparation des polysacharides microbians specifique. C. r. Seanc. Soc. Biol. Compt. rend.1993, 113, 490-496.
• 5. Collazos M. E., Ortega E., Barriga C.: Effect of temperature on the immune system of a cyprinid fish (Tinca tinca, L.). Blood phagocyte function at lowtemperature. Fish Shellfish Immunol. 1994b, 4, 231-238.
• 6. Dalmo R. A., Seljelid R.: The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(1,3)-d-glukan] on Atlantic Salmon, S. salar,macrophages in vitro. J. Fish Dis. 1995, 18, 175-185.
• 7. Dooley J. S. G., Lallier R., Shaw D. H., Trust T. J.: Electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains ofAeromonas hydrophila. J. Bacteriol. 1985, 164, 263-269.
• 8. Guz L.: Immunogenność szczepów z rodzaju Aeromonas. Doświadczalne szczepionki przeciwko MAI/MAS karpi. Rozprawy naukowe UP w Lublinie,ISSN 1899-2374, 2011, zeszyt 354.
• 9. Guz L., Sopińska A.: Wpływ β(1,3)-D-glukanu i lipopolisacharydu na odporność karpi przeciwko Aeromonas hydrophila. Med. Weter. 2009, 65, 715-718.
• 10. Karunasagar I., Rosalind G., Karunasagar I.: Immunological response of the Indian major carps to Aeromonas hydrophila vaccine. J. Fish Dis. 1991, 14,413-417.
• 11. Kawai K., Kusuda R.: Efficacy of the lipopolysacharide vaccine against vibriosis in cultured ayu. Bull. Japanese Soc. Scientific Fisheries 1983, 49,511-514.
• 12. Kozińska A.: Wskaźniki patogenności Aeromonas hydrophila, Aeromonas caviae i Aeromonas sobria. Rozprawa doktorska, 1996.
• 13. Kozinska A., Antychowicz J.: Immune cross reactions in carp (Cyprinus carpio L.) after single or double immunization with Aeromonas hydrophila andAeromonas sobria antigens. Bull. Vet. Inst. Puławy 2001, 45, 43-47.
• 14. Kozinska A., Antychowicz J.: Influence of an experimental Aeromonas hydrophila vaccine on selected hematological values and non-specific immunityin carp (Cyprinus carpio L.). Bull. Vet. Inst. Puławy 2000, 44, 169-178.
• 15. Kozinska A., Figueras M. J., Chacon M. R., Soler L.: Phenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp(Cyprinus carpio L.). J. Appl. Microbiol. 2002, 93, 1034-1041.
• 16. Kozińska A., Guz L.: The effect of various Aeromonas bestiarum vaccines on non-specific immune parameters and protection of carp (Cyprinus carpio L.).Fish Shellfish Immunol. 2004, 16, 437-445.
• 17. Lauve A., Dannevig B. H.: Fluid-phase endocytosis by rainbow trout head-kidney macrophages. Fish Shellfish Immunol. 1993, 3, 79-87.
• 18. Le Morvan C., Troutaud D., Deschaux P.: Differential effects of temperature on specific and non-specific immune defence in fish. J. Exp. Biol. 1998, 201,165-168.
• 19. Le Morvan C., Clerton P., Deschaux P., Troutaudf D.: Effects of environmental temperature on macrophage activities in carp. Fish Shellfish Immunol. 1997,7, 209-212.
• 20. Matthews E. S., Warriner J. E., Weeks B. A.: Assay of immune function in fish macrophages, techniques used as indicators of environemental stress, [w:]Stolen J. S., Fletcher T. C., Anderson D. P., Roberson B. S., Van MuiswikelW. B. (eds.): Techniques in fish immunology 1. SOS Publications, Fair Haven,N.J. 1990.
• 21. Nomura J., Aoki T.: Morphological analysis of lipopolysaccharide from Gram-negative fish pathogenic bacteria. Fish Pathol. 1984, 9, 193-197.
• 22. Raa J., Roerstad G., Engstad R., Robertsen B.: The use of immunostimulants to increase resistance of aquatic organisms to microbial infections. Dis. AsianAquaculture 1992, 1, 39-50.
• 23. Rowley A. F.: Collection, separation and identification of fish leukocytes, [w:] Stolen J. S., Fletcher T. C., Anderson D. P., Roberson B. S., van MuiswinkelW. B. (eds.): Techniques in fish immunology. Fair Haven (USA): SOS Publications 1990, 113-136.
• 24. Saeed M. O., Plumb J. A.: Immune response of channel catfish to lipopolysaccharide and whole cell Edward-siella ictaluri vaccines. Dis. Aquat. Org. 1986, 2, 21-25.
• 25. Salati F., Ikeda Y., Kusuda R.: Effect of Edwardsiella tarda lipopolysaccharide immunization on phagocytosis in the eel. Nippon Suisan Gakkaishi 1987, 53,201-204.
• 26. Selvaraj V., Sampath K., Sekar V.: Extraction and characterization of lipopolysaccharide from Aeromonas hydrophila and its effects on survival and hematology of the carp, Cyprinus carpio. Asian Fish. Sci. 2004, 17, 163-173.
• 27. Selvaraj V., Sampath K., Vaithilingam S.: Administration of lipopolysaccharide increases specific and non-specific immune parameters and survival in carp (Cyprinus carpio) infected with Aeromonas hydrophila. Aquaculture 2009, 286, 176-183.
• 28. Sinyakov M., Dror M., Margel M., Avtalion R. R.: Immunogenicity of Aeromonas salmonicida A-protein in goldfish (Carassius auratus L.). IsraeliJ. Aquacult-Bamidgeh 2001, 53, 110-114.
• 29. Siwicki A. K.: Immunostimulating influence of levamisole on non-specific immunity in carp (Cyprinas carpio). Dev. Comp. Immunol. 1989, 13, 87-91.
• 30. Solem S. T., Jorgensen J. B., Robertsen B.: Stimulation of respiratory burst and phagocytic activity in Atlantic salmon (Salmo salar, L.) macrophages bylipopolysaccharide. Fish Shellfish Immunol. 1995, 5, 475-491.
• 31. Sorensen K. K., Sveinbjornsson B., Dalmo R. A., Smedsrod B., Bertheussen K.: Isolation, cultivation and characterization of head kidney macrophages from Atlantic cod, Gadus morhua L. J. Fish Dis. 1997, 20, 93-107.
• 32. Studnicka M., Siwicki A., Ryka B.: Lysozyme level in carp (Cyprinus carpio L.). Bamidgen 1986, 38, 22-25.
• 33. Sychłowy A., Lukas A.: Ocena mikroilościowej metody redukcji NBT przez granulocyty krwi obwodowej. Polski Tygodnik Lekarski 1978, 33, 45-48.