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2008 | 26 | 4 |
Tytuł artykułu

Microstructure of porcine meat with the Rendement Napole gene

Treść / Zawartość
Warianty tytułu
PL
Mikrostruktura mięśni świń z genem kwaśnego mięsa (RN)
Języki publikacji
EN
Abstrakty
EN
The Rendement Napole gene (RN) consists of a mutation at the γ3 subunit of AMP-activated protein kinase (AMPK) in pigs. AMPK works as an energy level sensor and controls numerous cellular pathways. The study aimed at identifying the differences in microstructure occurring between the normal porcine meat and porcine meat encoded by the RN gene. Using molecular techniques 194 commercial pigs were sampled to determine their genotypes. Meat from the semimembranosus muscle was examined using optic (OM) and transmission electron microscopy (TEM). The genotypes rnI/rnI, rnV/rnV, rnV/rnI, RN/rnV and RN/rnI were identified. OM revealed broadening of the sarcoplasmic space in RN animals, while TEM showed disordering of muscle fibres and alteration of the sarcomeric structure. Mitochondrial degradation was found in both RN and normal animals,however, the morphological changes observed seemed to be caused by the mutated AMPK.
PL
Gen kwaśnego mięsa (Rendement Napole – RN) jest wynikiem mutacji w podjednostce γ3 kinazy białkowej aktywowanej przez AMP (AMPK) świń. AMPK działa jako czujnik poziomu energii i kontroluje szereg ścieżek metabolizmu komórki. Badanie miało na celu identyfikację różnic mikrostrukturalnych w budowie mięśniówki normalnych i noszących gen RN. Wykorzystując techniki molekularne zgenotypowano 194 świnie z fermy produkcyjnej. Próbki mięśnia półbłoniastego (musculus semimembranosus) badano za pomocą mikroskopu optycznego (OM) i elektronowego mikroskopu transmisyjnego (TEM). Zidentyfikowano następujące genotypy: rn1/n1, rnV/rnV, rnV/rn1, RN/rnV i RN/rn1. Badanie OM ujawniło poszerzenie przestrzeni sarkoplazmatycznej u zwierząt z RN, podczas gdy badanie TEM wykazało dezorganizację włókien mięśniowych i zmianę struktury sarkomerycznej. Degradację (zwyrodnienie) mitochondrialną stwierdzono niezależnie od genotypu RN jednak obserwowane zmiany morfologiczne wydają się spowodowane zmutowaną AMPK.
Wydawca
-
Rocznik
Tom
26
Numer
4
Opis fizyczny
p.259-267,fig.,ref.
Twórcy
  • Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico
autor
  • Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico
autor
  • Faculty of Medicine, Universidad Autonoma de Chihuahua, Av. Colon 1003 Chihuahua, Chih. 31350, Mexico
autor
  • Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico
autor
  • Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico
autor
  • Faculty of Medicine, Universidad Autonoma de Chihuahua, Av. Colon 1003 Chihuahua, Chih. 31350, Mexico
  • Department of Veterinary Sciences, Biomedical Sciences Institute, Universidad Autonoma de Cd. Juaez, Henri Dunant 4016, Cd. Juarez, Chih. 32310, Mexico
Bibliografia
  • 1. ANDERSSON L., 2003 – Identification and characterization of AMPKγ3 mutations in the pig.Biochemical Society Transactions 31, 232-235.
  • 2. BARTON E.R., MORRIS L., MUSARO A., ROSENTHAL N., SWEENEY H.L., 2002 – Musclespecific expression of insulin-like growth factor I counters muscle decline in mdx mice. Journal of
  • Cell Biology 157, 137-147.
  • 3. CIOBANU D., BASTIAANSEN J., MALEK M., HELM J., WOOLLARD J., PLASTOW G.ROTHSCHILD M., 2001 – Evidence for new alleles in the protein kinase adenosine monophosphateactivated .3-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality. Genetics 159, 1151-1162.
  • 4. DENG Y., ROSENVOLD K., KARLSSON A .H., HORN P., HEDEGAARD J., STEFFENSEN C.L., ANDERSEN H.J., 2002 – Relationship between thermal denaturation of porcine muszle proteins and water-holding capacity. Journal of Food Science 67, 1642-1647.
  • 5. ESTRADE M., AYOUB S., TALMANT A., MONIN G., 1994 – Enzyme activities of glycogen metabolism mitochondrial characteristics in muscles of RN-carrier pigs (Sus scrofa domesticus)Comparative Biochemistry, Physiology B. 108B, 295-301.
  • 6. ESTRADE M., VIGNON X., MONIN G., 1993a – Effect of the RN gene on ultrastructure protein fraction in pig muscle. Meat Science 35, 313-319.
  • 7. ESTRADE M., VIGNON X., ROCK E., MONIN G., 1993b – Glycogen hyperaccumulation in white muscle fibres of RN-carrier pigs. A biochemical ultrastructural study. Comparative Biochemistry,Physiology B. 104B, 321-326.
  • 8. FERNANDEZ X., MONIN G., 1994 – A major gene affecting pork quality, the RN gene. Meat Focus International 3(8), 332-334.
  • 9. HAMILTON D.N., ELLIS M., MILLER K.D., MCKEITH F.K., PARRETT D.F., 2000 – The effect of the Halothane and Rendement Napole genes on carcass and meat quality characteristics of pigs. Journal of Animal Science 78, 2862-2867.
  • 10. HARDIE D.G., 2004 – AMP-activated protein kinase, the guardian of cardiac energy status. Journal of Clinical Investigation 114, 465-468.
  • 11. HARPER S.Q., HAUSER M.A., DELLORUSSO C., DUAN D., CRAWFORD R.W., PHELPS S.F., HARPER H.A., ROBINSON A.S., ENGELHARDT J.F., BROOKS S.V., CHAMBERLAIN J.S., 2002 – Modular flexibility of dystrophin, implications for gene therapy of Duchenne muscular dystrophy. Nature Medicine 8, 253-261.
  • 12. JOSELL Ĺ., MARTINSSON L., BOGAARD C., ANDERSEN J.R., TORNBERG E., 2000 –Determination of RN phenotype in pigs at slaughter-line using visual and near-infrared spectroscopy.Meat Science 55, 273-278.
  • 13. LUNDSTRÖM K., ANDERSSON A., HANSSON I., 1996 – Effect of the RN gene on technological and sensory meat quality in crossbred pigs with Hampshire as terminal sire. Meat Science 42, 145–153.
  • 14. LUNDSTRÖM K., ENFÄLT A.C., TORNBERG E., AGERHEM H., 1998 – Sensory and technological meat quality in carriers of the RN- allele in Hampshire crosses and in purebred Yorkshire pigs. Meat Science 48, 115-124.
  • 15. MEADUS W.J., MCINNIS R., DUGAN M.E.R., 1998 – The frequency of the PRKAG3 mutation (RN-) in the commercial pig population and its’ cost to ham production, At: http //www.cpc-ccp.com/res/frequency_of_the_prkag3_mutation .htm, Consulted 23 September 2003.
  • 16. MILAN D., JEON J.T., LOOFT C., ARMAGER V., ROBIC A., THELANDER M., ROGELGAILLARD C., PAUL S., IANNUCCELLI N., RASK L., RONNE H., LUNDSTRÖM K., REINSCH N., GELLIN J., KALM E., LE ROY P., CHARDON P., ANDERSSON L., 2000 – A mutation In PRKAG3 associated with excess glycogen content in pig skeletal muscle. Science 288, 1248-1251.
  • 17. MOELLER S.J., BAAS T.J., LEEDS T.D., EMNETT R.S., IRVIN K.M., 2003 – Rendement Napole gene effects and a comparison of glycolytic potential and DNA genotyping for classification of Rendement Napole status in Hampshire sired pigs. Journal of Animal Science 81, 402-410.
  • 18. MONIN G., SELLIER P., 1985 – Pork of low technological quality with a normal rate of muszle pH fall in the immediate postmortem period. The case of the Hampshire breed. Meat Science 13,49-63.
  • 19. MU J., BARTON E.R., BIRNBAUM M.J., 2003 – Selective suppression of AMP-activated protein kinase in skeletal muscle, update on ‘lazy mice’. Biochemical Society Transactions 31, 236-241.
  • 20. PEARSON A.M. YOUNG R.B., 1989 – Muscle and Meat Biochemistry. Academic Press, New York, pp 457
  • 21. ROBERTS R.G., 2001 – Dystrophins and dystrobrevins. Genome Biology 2, 1-7.
  • 22. RUDBECK L., DISSING J., 1998 – Rapid, simple alkaline extraction of human genomic DNA from whole blood, buccal epithelial cells, semen and forensic stains for PCR. Biotechniques 25,588-592.
  • 23. SCOTT J.W., HAWLEY S.A., GREEN K.A., ANIS M., STEWART G., SCULLION G.A.,NORMAN D.G., HARDIE D.G., 2004 – CBS domains form energy sensing modules whose binding of adenosine ligands is disrupted by disease mutations. Journal of Clinical Investigation 113, 274-284.
  • 24. WANG J., SILVA J.P., GUSTAFSSON C.M., RUSTIN P., LARSSON N.G. 2001 – Increased In vivo apoptosis in cells lacking mitochondrial DNA gene expression. Proceedings of the National Academy of Sciences of the USA 98, 4038-4043.
  • 25. YUAN X.M., LI W., DALEN H., LOTEM J., KAMA R., SACHS L. BRUNK U.T. 2002 – Lysosomal destabilization in p53-induced apoptosis. Proceedings of the National Academy of Sciences of the USA 99, 6286-6291.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-08d919c1-1fe6-4e84-baad-76002b1b6c21
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