Microstructure of porcine meat with the Rendement Napole gene

• Autorzy: Alarcon-Rojo A.D. , Zapata I. , Solis F. J. , Lara I. , Burrola E. , Cordoba L. , Janacua-Vidales H.

Warianty tytułu

PL

Mikrostruktura mięśni świń z genem kwaśnego mięsa (RN)

• Języki publikacji: EN

Abstrakty

EN

The Rendement Napole gene (RN) consists of a mutation at the γ3 subunit of AMP-activated protein kinase (AMPK) in pigs. AMPK works as an energy level sensor and controls numerous cellular pathways. The study aimed at identifying the differences in microstructure occurring between the normal porcine meat and porcine meat encoded by the RN gene. Using molecular techniques 194 commercial pigs were sampled to determine their genotypes. Meat from the semimembranosus muscle was examined using optic (OM) and transmission electron microscopy (TEM). The genotypes rnI/rnI, rnV/rnV, rnV/rnI, RN/rnV and RN/rnI were identified. OM revealed broadening of the sarcoplasmic space in RN animals, while TEM showed disordering of muscle fibres and alteration of the sarcomeric structure. Mitochondrial degradation was found in both RN and normal animals,however, the morphological changes observed seemed to be caused by the mutated AMPK.

PL

Gen kwaśnego mięsa (Rendement Napole – RN) jest wynikiem mutacji w podjednostce γ3 kinazy białkowej aktywowanej przez AMP (AMPK) świń. AMPK działa jako czujnik poziomu energii i kontroluje szereg ścieżek metabolizmu komórki. Badanie miało na celu identyfikację różnic mikrostrukturalnych w budowie mięśniówki normalnych i noszących gen RN. Wykorzystując techniki molekularne zgenotypowano 194 świnie z fermy produkcyjnej. Próbki mięśnia półbłoniastego (musculus semimembranosus) badano za pomocą mikroskopu optycznego (OM) i elektronowego mikroskopu transmisyjnego (TEM). Zidentyfikowano następujące genotypy: rn1/n1, rnV/rnV, rnV/rn1, RN/rnV i RN/rn1. Badanie OM ujawniło poszerzenie przestrzeni sarkoplazmatycznej u zwierząt z RN, podczas gdy badanie TEM wykazało dezorganizację włókien mięśniowych i zmianę struktury sarkomerycznej. Degradację (zwyrodnienie) mitochondrialną stwierdzono niezależnie od genotypu RN jednak obserwowane zmiany morfologiczne wydają się spowodowane zmutowaną AMPK.

Słowa kluczowe

EN

microstructure; pork meat; muscle; meat; Rendement Napole gene zob.RN gene; RN gene

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2008
• Tom: 26
• Numer: 4
• Opis fizyczny: p.259-267,fig.,ref.

Twórcy

autor

Alarcon-Rojo A.D.

• Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico

autor

Zapata I.

• Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico

autor

Solis F. J.

• Faculty of Medicine, Universidad Autonoma de Chihuahua, Av. Colon 1003 Chihuahua, Chih. 31350, Mexico

autor

Lara I.

• Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico

autor

Burrola E.

• Faculty of Animal Science, Universidad Autonoma de Chihuahua, Periferico Fco. R. Almada km 1, Chihuahua, Chih. 31031, Mexico

autor

Cordoba L.

• Faculty of Medicine, Universidad Autonoma de Chihuahua, Av. Colon 1003 Chihuahua, Chih. 31350, Mexico

autor

Janacua-Vidales H.

• Department of Veterinary Sciences, Biomedical Sciences Institute, Universidad Autonoma de Cd. Juaez, Henri Dunant 4016, Cd. Juarez, Chih. 32310, Mexico

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