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2010 | 32 | 4 |
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Biochemical and enzymatic changes in rice plants as a mechanism of defense

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A laboratory study was undertaken to ascertain the impact and the extent of feeding by different pests on biochemical constituents and various enzyme levels in rice plants. The difference in these parameters due to the pest damage by three different modes of feeding was also studied and compared. The borer pest—yellow stem borer (YSB), Scirpophaga incertulas (W); surface feeder—leaf roller (LR), Cnaphalocrosis medinalis (G) and a sucking pest—brown plant hopper (BPH), Nilaparvata lugens (S) fed rice plants were analyzed for the quantitative and qualitative changes in biochemical profile and enzymatic changes that occur as plant’s defensive responses were analyzed spectrophotometrically. The phenolic acids were analyzed using HPLC and quantitated with the standard samples. The quantity of biochemicals such as proteins, phenols and carbohydrates has been enhanced along with the enzyme activities of peroxidase (POD), catalase (CAT), chitinase (CHI). A decrease in superoxide dismutase (SOD), phenyl alanine ammonia lyase (PAL), β-1, 3-glucanase (GLU) enzyme activities were evident in pest infested plants. Phenolic acids like vanillic acid, syringic acid, cinnamic acid, and p-coumaric acids were mostly found in the infested plants. We demonstrate that the elevated levels of biochemicals, phenolic acids, and enzymes may play a major role in plant defense.
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  • Biology and Biotechnology Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Andhra Pradesh 500607, India
  • Biology and Biotechnology Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Andhra Pradesh 500607, India
  • Aebi H (1984) Catalase. In: Packer L (ed) Methods in enzymology. Academic press, Orlando, pp 121–126
  • Baldwin IT (1994) Chemical changes rapidly induced by folivory. In: Bernays EA (ed) Insect–plant interactions, vol 5. CRC Press, Boca Raton, pp 1–23
  • Beyer W, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566
  • Bi JL, Felton GW (1995) Foliar oxidative stress and insect herbivory: primary compounds, secondary metabolites and reactive oxygen species as components of induced resistance. J Chem Ecol 21:1511–1530
  • Chen Z, Silva H, Klessig DF (1993) Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science 262:1883–1886
  • Chittoor JM, Leach JE, White FF (1999) Induction of peroxidase during defense against pathogens. In: Datta SK, Muthukrisnan S (eds) Pathogenesis-related proteins in plants. CRC Press, Boca Raton, pp 171–193
  • Constabel CP (1999) A survey of herbivore-inducible defensive proteins and phytochemicals. In: Agrawal AA, Tuzun S, Bent E (eds) Induced plant defenses against herbivores and pathogens. APS Press, St. Paul, pp 137–166
  • Dickerson DP, Pascholati SF, Hagerman AE, Butler LG, Nicholson RL (1984) Phenyl alanine lyase and hydroxycinnamate: CoA ligase in maize mesocotyls inoculated with Helminthosporium carbonum. Physiol Plant Pathol 25:111–123
  • Dixon RA, Pavia NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097
  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
  • Felton GW, Donato KK, Broadway RM, Duffey SS (1992) Impact of oxidized plant phenolics one the nutritional quality of dietary protein to a noctuid herbivore. J Insect Physiol 38:277–285
  • Flinn PW, Hower AA, Knievel DP (1990) Physiological response of alfalfa to injury by Empoasca fabae (Homoptera: Cicadallidae). Environ Entomol 19:176–181
  • Haukioja E, Niemela P (1977) Retarded growth of a geometrid larva after mechanical damage to leaves of its host tree. Ann Zool Fenn 14:48–52
  • Hildebrand DF, Rodriquez JG, Brown GC, Luu KT, Volden CS (1986) Peroxidative responses of leaves in two soybean genotypes injured by two spotted spider mite (Acari: Tetranychidae). Appl Entomol Zool 20:348–349
  • Hori K (1973) Studies on the feeding habits of Lygus disponsi Linnavuori (Hemiptera: Mirides) and the injury to its host plant. III. Phenolic compounds, acid phosphatase and oxidative enzymes in the injured tissue of sugar beet leaf. Appl Entomol Zool 8:103–112
  • Inbar M, Doostdar H, Leibee GL, Mayer RT (1999) The role of plant rapidly induced responses in asymmetric interspecific interactions among insect herbivores. J Chem Ecol 25:1961–1979
  • Ito H, Tsugawa H, Matsui H, Honma M, Otsuki Y, Murakami T, Ohashi Y (2000) Xylem-specific expression of wound-inducible rice peroxidase genes in transgenic plants. Plant Sci 155:85–100
  • Johnson R, Narvaez J, An G, Ryan C (1989) Expression of proteinase inhibitors I and II in transgenic tobacco plants: effects on natural defense against Manduca sexta larvae. Proc Natl Acad Sci USA 86:9871–9875
  • Jwa NS, Agrawal GK, Tamogami S, Yonekura M, Han O, Iwahashi H, Rakwal R (2006) Defense/stress-related marker genes, proteins and secondary metabolites in defining rice self-defense mechanisms. Plant Physiol Biochem 44:261–273
  • Jyothsna Y, Kapil M, Usha Rani P (2009) Effects of herbivore feeding on biochemical and nutrient profile of castor bean, Ricinus communis L. plants. Allelopathy J 24(1):131–142
  • Kar M, Mishra D (1976) Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. Plant Physiol 57:315–319
  • Karban R, Baldwin IT (1997) Induced responses to herbivory. University of Chicago Press, Chicago
  • Kogan M, Paxton J (1983) Natural inducers of plant resistance to insects. In: Hedin PA (ed) Plant resistance to insects. American Chemical Society Symposium. Series 208 American Chemical Society, Washington, DC, pp 153–171
  • Lee DH, Kim YS, Lee CB (2001) The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). J Plant Physiol 158:737–745
  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
  • Mayer RT, Mc Collum TG, Mc Donald RE, Polston JE, Doostdar H (1996) Bemisia feeding induces pathogenesis-related proteins in tomato. In: Gerling D, Mayer RT (eds) Bemisia 1995: taxonomy, biology, damage control and management. Intercept Ltd., Andover, pp 179–188
  • Miller GL (1959) Use of dinitrosalicylic acid for estimation of reducing sugar. Anal Chem 31:426–434
  • Mohase L, van der Westhuizen A (2002) Salicylic acid is involved in resistance responses in the Russian wheat aphid–wheat interaction. J Plant Physiol 159:585–590
  • Oidaira H, Sano S, Koshiba T, Ushimaru T (2000) Enhancement of antioxidative enzyme activities in chilled rice seedlings. J Plant Physiol 156:811–813
  • Orozco-Cardenas M, Ryan CA (1999) Hydrogen peroxide is generated systematically in plant leaves by wounding and systemin via the octadecanoid pathway. Proc Natl Acad Sci USA 96: 6553–6557
  • Pan SQ, Ye XS, Kué J (1991) A technique for detection of chitinase, β-1, 3-glucanases, and protein patterns after a single separation using polyacrylamide gel electrophoresis or isoelectrofocusing. Phytopathology 81:970–973
  • Pathak MD (1969) Resistance to Nephotettix impicticeps and N. lugens in varieties of rice. Nature 223:502–504
  • Pathak MD, Saxena RC (1980) Breeding approaches in rice. In: Smith H (ed) Breeding plants resistant to insects. Pergamon, Oxford, pp 61–81
  • Ryan CA, Bishop PD, Walker-Simmons M, Brown WE, Graham JS (1985) Pectic fragments regulate the expression of proteinase inhibitor genes in plants. In: Key J, Kosuge T (eds) NS, UCLA Symposia on molecular and cellular biology, vol 22. Liss Inc., New York, pp 319–334
  • Shah K, Kumar RG, Verma S, Dubey RS (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedling. Plant Sci 161:1135–1144
  • Shapiro M, Preisler HK, Robertson JL (1987) Enhancement of baculovirus activity on gypsy moth (Lepidoptera: Lymantriidae) by chitinase. J Econ Entomol 80:1113–1116
  • Sogawa K (1971) Effects of feeding of the brown plant hopper on the components in the leaf blade of rice plants. Jpn J Appl Entomol Zool 15:175–179
  • Stout MJ, Fidantsef AL, Duffey SS, Bostock RM (1999) Signal interactions in pathogen and insect attack: systemic plant-mediated interactions between pathogens and herbivores of the tomato, Lycopersicon esculentum. Physiol Mol Plant Pathol 54:115–130
  • Usha Rani P, Jyothsna Y (2009) Physiological changes in the groundnut,Arachis hypogaeaL. plant due to the infection of a fungal pathogen, Cercosporidium personatum Deighton. Allelopathy J 23(2):369–378
  • Usha Rani P, Kumari SI, Sriramakrishna T, Sudhakar TR (2007) Kairomones extracted from rice yellow stem borer and their influence on egg parasitization by Trichogramma japonicum Ashmead. J Chem Ecol 33:59–73
  • Wang X, Ding X, Gopalakrishnan B, Morgan TD, Johnson L, White EFF, Muthukrishnan S, Kramer KJ (1996) Characterization of a 46 kDa insect chitinase from transgenic tobacco. Insect Biochem Mol Biol 26:1055–1064
  • Watanabe T, Kitagawa H (2000) Photosynthesis and translocation of assimilates in rice plants following phloem feeding by the plant hopper Nilaparvata lugens (Homoptera: Delphacidae). J Econ Entomol 93:1192–1198
  • Wold EN, Marquis RJ (1997) Induced defense in white oak: effects on herbivores and consequences for the plant. Ecology 78:1356–1369
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