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2017 | 73 | 06 |
Tytuł artykułu

Ocena zdolności zapładniającej nasienia buhajów w oparciu o analizę in vivo i in vitro

Treść / Zawartość
Warianty tytułu
EN
Assessment of fertilizing capacity of bull semen based on in vivo and in vitro assays
Języki publikacji
PL
Abstrakty
EN
The aim of this work was to evaluate the fertilizing capacity of bulls on the basis of the assessment of fresh and thawed semen, following the swim-up procedure and immunological assay with IgA and IgG antisperm antibodies. In addition, the suitability of immunological reaction with IgG and IgA antisperm antibodies for seminological assessment of bull semen was examined. Tests were conducted with semen originating from Holstein-Friesian bulls. Ejaculates were collected twice from 15 two-year-old males, yielding 30 samples of semen. After the calculation of sperm concentration and motility, the samples were diluted with Biociphos-Plus® and frozen in straws. After thawing, sperm concentration and motility were calculated again (an average of 101.4 million sperm/ml with a motility of around 21%), and the spermatozoa were subjected to swim-up. At the same time, frozen-thawed bull semen was tested for the presence of antisperm antibodies, using SpermMar Tests for IgG and IgA. According to the records of the insemination centre, 53 515 insemination treatments had been conducted with semen of selected bulls. In a population of 30 324 cows, the calculated insemination index amounted to 1.76. Half of the cows which were qualified for insemination had already been fertilized with the first treatment (ca 52.8%). After the swim-up, there was an average of 4.5 million hyperactivated spermatozoa with a motility of around 41%. Furthermore, spermatozoa associated with IgG antisperm antibodies were found in 20 ejaculates, i.e. in around 66.67% of all semen samples obtained. The spermatozoa reacted positively with IgA antibodies in as many as 23 samples, which constituted 76.67% of all ejaculates obtained. Correlations between the routinely analyzed semen parameters and the selected parameters (immunological, swim-up) which were demonstrated in this work, did not appear to be sufficiently convincing. Therefore, it seems necessary to repeat this study with a population of clinically healthy, fertile bulls, as well as with juvenile bulls newly introduced to the insemination centre or with bulls with reduced fertility.
Wydawca
-
Rocznik
Tom
73
Numer
06
Opis fizyczny
s.357-361,rys.,tab.,bibliogr.
Twórcy
autor
  • Katedra Biochemii i Biotechnologii Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, ul. Mazowiecka 28, 85-084 Bydgoszcz
autor
  • Katedra Biochemii i Biotechnologii Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, ul. Mazowiecka 28, 85-084 Bydgoszcz
autor
  • Katedra Biochemii i Biotechnologii Zwierząt, Wydział Hodowli i Biologii Zwierząt, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, ul. Mazowiecka 28, 85-084 Bydgoszcz
Bibliografia
  • Byrd W., Bradshaw K., Carr B., Edman C., Odom J., Ackerman G.: A prospective randomized study of pregnancy rates following intrauterine and intracervical insemination using frozen donor sperm. Fertil. Steril. 1990, 53, 521-527.
  • Fargeas E.: Quelques resultats obtenus en insemination artificielle avec la semence congelee. Cuniculture 1995, 22, 103-106.
  • Frazer L. R.: Requirements for successful mammalian spermatozoa capacitation and fertilization. Arch. Path. Lab. Med. 1992, 116, 345-350.
  • Gadea J.: Sperm factors related to in vitro and in vivo porcine fertility. Theriogenology 2005, 63, 431-444.
  • Hallap T., Håård M., Jaakma U., Larsson B., Rodriguez-Martinez H.: Does cleansing of frozen-thawed bull semen before assessment provide samples that relate better to potential fertility? Theriogenology 2004, 62, 702-713.
  • Janicki B., Solińska J., Łakota P.: Evaluation of dog semen quality using a conventional microscopic method, flow cytometry and HTM IVOS computer sperm quality analyzer. Med. Weter. 2006, 62, 1076-1079.
  • Januskauskas A., Zilinskas H.: Bull semen evaluation post thaw and relation of semen characteristics to bull’s fertility. Vet. Zootec. 2002, 17, 29-36.
  • Klimowicz M., Niżański W., Savic M. A., Zbyryt I., Dubiel A.: Evaluation of dog semen quality using a conventional microscopic method, flow cytometry and HTM IVOS computer sperm quality analyzer. Med. Weter. 2005, 61, 1250-1255.
  • Kratz E. M., Achcińska M. K.: Molecular mechanisms of fertilization: the role of male factor). Post. Hig. Med. Dosw. 2011, 65, 784-795.
  • Kratz E. M., Pupek M., Chełmońska-Soyta A., Kątnik-Prastowska I.: The Molecular Forms of Immunoglobulin A in Human Seminal Plasma. Adv. Clin. Exp. Med. 2004, 13, 541-547.
  • Mehmood A., Anwar M., Saqlan Naqvi S. M.: Motility, acrosome integrity, membrane integrity and oocyte cleavage rate of sperm separated by swim-up or Percoll gradient method from frozen–thawed buffalo semen. Anim. Reprod. Sci. 2009, 111, 141-148.
  • Mikołajczyk M., Baj Z.: Occurrence of the antisperm antibodies in cryptorchidism and their role in infertility induction. Diagn. Lab. 2008, 44, 261-268.
  • Mocé E., Lavara R., Vicente J. S.: Effect of cooling rate to 5°C, straw size and farm on fertilizing ability of cryopreserved rabbit sperm. Reprod. Domest. Anim. 2010, 45, 1-7.
  • Sardoy M. C., Anderson D. E., George A., Wilkerson M. J., Skinner S., Ferrer M. S.: Standardization of a method to detect bovine sperm-bound antisperm antibodies by flow cytometry. Theriogenology 2012, 78, 1570-1577.
  • Sharma M., Singh M., Kapoor S., Jasial S.: Inter relationship between some routine semen evaluation parameters in Jersey X local hill cattle crossbred bulls. Open Vet. J. 2012, 2, 26-31.
  • Verstegen J., Iguer-ouada M., Onclin K.: Computer assisted semen analyzers in andrology and veterinary practice. Theriogenology 2002, 57, 149-179.
  • Watson P. F.: The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 2000, 60-61, 481-492.
  • Watson P. F.: The effect of cold shock on sperm cell membranes, [w:] Morris G. J., Clarke A.: Effect of Low Temperatures on Biological Membranes. Academic Press, London 1981, 187-218.
  • Wyrzykowska R., Domagała A., Kurpisz M.: Molecular factor in reproduction. The role and characteristics of antisperm antibodies. Post. Biol. Kom. 2005, 32, 537-548.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-07bd8068-0385-4ca1-afa5-6c62676b902b
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