Identification of Brucella DNA in lymph tissue from deer (Cervus elaphus) and wild boars (Sus scrofa) by the use of BCSP31 PCR and AMOS-PCR

• Autorzy: Weiner M. , Iwaniak W. , Szulowski K.
• Języki publikacji: EN

Abstrakty

EN

At first, a genus-specific PCR assay (BCSP31) to identify all known Brucella, generating an amplicon of 223 bp, was used. Then, positive samples were analysed with AMOS-PCR, which enables us to identify selected biovars of B. abortus, B. melitensis, B. ovis, and B. suis. The BCSP31 PCR analysis revealed that 27 (11.5%) of 235 samples originating from wild boars were positive and they all were negative in AMOS-PCR. Twenty-five were identified as B. suis biovar 2 through bacteriological examination. On the other hand, none of the 183 samples from deer was positive in PCR or bacteriological examination. The obtained results can provide a better knowledge of the potential reservoirs of infection and also should lead to great progress in molecular epidemiology.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.609-612,fig.,ref.

Twórcy

autor

Weiner M.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Iwaniak W.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Szulowski K.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

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