Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 21 | 6 |

Tytuł artykułu

Biodegradation and characterization studies of different kinds of polyurethanes with several enzyme solutions

Warianty tytułu

Języki publikacji



In the present work, biodegradation behaviors compared commercial polyurethane to synthesized polyurethane in different enzyme solutions. Two quantitative methods have been used to follow the biodegradation. Enzymes were esterase and protease in the hydrolase class; enzyme solutions were prepared one by one or by combining as enzyme cocktail solutions. Esterase affect ester bonds whereas protease enzymes affect urethane and urea linkages. Thus, effects of enzyme cocktail solutions were also researched in this study. It is a new way for in vitro degradation of polyurethane samples by enzyme cocktail solutions. 1cm×1cm of polyurethane films were put in a tube and treated by different combinations of these mentioned enzymes. The tubes were placed in a shaker and stirred at 120 rpm under the reaction condition of 37ºC and pH 7.4. In order to keep enzymatic activity stable, enzyme solutions were prepared again periodically. All samples were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. The bond strength of chains and morphological changes were recorded during the period of biodegradation. The results showed that protease enzyme solutions could erode polymer films, but esterase enzyme solution could not be so effective alone. In addition, enzyme cocktail solutions showed that enzymes could compete with each other, so one enzyme could suppress the activity of another enzyme.

Słowa kluczowe








Opis fizyczny



  • Chemical and Metallurgical Engineering Faculty, Chemical Engineering Department, Istanbul Technical University, 34469 Maslak-Istanbul, Turkey
  • Chemical and Metallurgical Engineering Faculty, Chemical Engineering Department, Istanbul Technical University, 34469 Maslak-Istanbul, Turkey
  • Chemical and Metallurgical Engineering Faculty, Chemical Engineering Department, Istanbul Technical University, 34469 Maslak-Istanbul, Turkey


  • 1. HOWARD G. T., BLAKE R. C. Growth of Pseudomonas fluorescens on a polyester-polyurethane and the purification and characterization of a polyurethanase-protease enzyme. Int. Biodet. Biodeg. 42, 213, 1998.
  • 2. EDLUND U., ALBERTSSON A. C. Polyesters based on diacid monomers. Adv. Drug Del. Rev. 55, 585, 2003.
  • 3. KLOSS J. R., PEDROZO T. H., FOLLMANN H. D. M., PERALTA-ZAMORA P., DIONISIO J. A., AKCELRUD L., ZAWADZKI S.F. Application of the principal component analysis method in the biodegradation polyurethanes evaluation. Mat. Sci. Eng. 29, 470, 2009.
  • 4. POK S. W., WALLACE K. N., MADIHALLY S. V. In vitro characterization of polycaprolactone matrices generated in aqueous media. Acta Biom. 6, 1061, 2010.
  • 5. KHARE A., DESMUKH S. Studies toward producing ecofriendly plastics. J. Plastic Film Sheeting 22, 193, 2006.
  • 6. HOWARD G. T. Biodegradation of polyurethane: a review. Int. Biodet. Biodeg. 49, 245, 2002.
  • 7. KEDZIEREWICZ F., THOUVENOT P., MONOT I., HOFFMAN M., MAINCENT P. Influence of different physicochemical conditions on the release of indium oxine from nanocapsules. J. Biomed. Mat. Res. 39, 588, 1998.
  • 8. CHRISTENSON E. M., PATEL S., ANDERSON J. M., HILTNER A. Enzymatic degradation of poly(ether urethane) and poly(carbonate urethane) by cholesterol esterase. Biom. 27, 3920, 2006.
  • 9. SARKAR D., LOPINA S. T. Oxidative and enzymatic degradations of L-tyrosine based polyurethanes. Polym. Deg. Sta. 92, 1994, 2007.
  • 10. YANG H., LOPINA S. T. In vitro enzymatic stability of dendritic peptides. J. Biom. Mat. Res. Part A. 76, 398, 2006.
  • 11. FUKUSHIMA K., TABUANI D., ABBATE C., ARENA M., FERRERI L. Effect of sepiolite on the biodegradation of poly(lactic acid) and polycaprolactone. Poly. Deg. Sta. 95, 2049, 2010.
  • 12. KIM Y. D., KIM S. C. Effect of chemical structure on the biodegradation of polyurethanes under composting conditions. Poly. Deg. Sta. 62, 343, 1998.
  • 13. GAUTAM R., BASSI A. S., YANFUL E. K. Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium. Biotech. Let. 29, 1081, 2007.
  • 14. UMARE S. S., CHANDURE A. S. Synthesized, characterization and biodegradation studies of poly(ester urethane)s. Chem. Eng. J. 142, 65, 2008.
  • 15. YAMAMOTO N., NAKAYAMA A., OSHIMA M., KAWASAKI N., AIBA S. Enzymatic hydrolysis of lysine diisocyanate based polyurethane ureas by various proteases. React. Func. Polym. 67, 1338, 2008.
  • 16. GÖPFERICH A. Mechanisms of polymer degradation and erosion. Biom. 17, 103, 1996.
  • 17. TSUJI H., IKARASHI K. In vitro hydrolysis of poly(L-lactide) crystalline residues as extended-chain crystallites III. Effects of pH and enzyme. Polym. Deg. Sta. 85, 647, 2004.
  • 18. WANG Y., LIU L., GUO S. Characterization of biodegradable and cytocompatible nano-hydroxyapatite/polycaprolactone porous scaffolds in degradation in vitro. Polym. Deg. Sta. 95, 207, 2010.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.