Potentially therapeutic neural stem cell line from human cord blood (HUCB-NSC) has been established in our laboratory. Reaching appropriate target by transplanted cells is a prerequisite for success of cell therapy for stroke. The question arises what is the migration potential of HUCB-NSC towards infarcted brain tissue. The migration of HUCB-NSC towards rat tissue homogenates from healthy brain (THHB) and ouabain-induced focal brain injury (THIB) obtained 6 h, 48 h and 7 days after insult was studied in vitro using transwells. Additionally the migratory activities of HUCB-NSC was checked in the presence of migration inducing proteins IGF-1 (200 ng/ml) and SDF-1 (10 ng/ml) dissolved in culture medium. Immunocytochemical analysis of migration-related receptors (CXCR-4, IGF-1R) on HUCB-NSC was performed. Results: Immunohistochemistry of HUCB-NSC unveiled expression of CXCR-4, IGF-1R. HUCB-NSC revealed robust migration toward THIB in comparison to THHB, which was most pronounced in the presence of 48 h postinfarct brain tissue (900 vs. 300 cells/well, P<0.05). The presence of IGF-1 and SDF-1 in medium increased signifi cantly HUCB-NSC migration but the effect was much weaker in comparison to injured brain tissue. The ability of robust in vitro migration of HUCB-NSC towards infarcted rat brain tissue has been confi rmed. Neither IGF-1 nor SDF-1 seems to play a pivotal role in this lesion-induced migration of HUCB-NSC. Supported by MSHE grant: N N401 332636.