Characterizing microbial populations in petroleum-contaminated soils of Swat district, Pakistan

• Autorzy: Uddin M.N. , Ali M. , Muhammad M. , Farooq M. , Ahmad N. , Jamil J. , Kalsoom J.J. , Adnan M. , Shah N. , Khan A.
• Języki publikacji: EN

Abstrakty

EN

Soil samples from petroleum-contaminated soil were collected from 25 different petroleum filling stations and automobile workshops in the district of Swat, Khyber Pakhtunkhawa, Pakistan. A total of seven bacterial genera were isolated. All of the isolates were Gram-positive bacteria. The genera identified by the culture and cell morphological characteristics were: Bacillus, Streptococcus, Staphylococcus, Micrococcus, Corynebacterium Arthrobacter, and Streptomyces. Lipolytic and saline activities of the selected isolates were studied. Among the isolates, Arthrobacter, Staphylococcus, Bacillus, Micrococcus, Corynebacterium, and Streptomyces produced lipase enzymes, while no lipase was produced by Streptococcus. Dense growth of Bacillus and Streptococcus was observed at 1% NaCl. Dense growth of Streptomyces was observed at strength of 2% NaCl. At 3% NaCl concentration, dense growth of Staphylococcus, Micrococcus, Corynebacterium, and Arthrobacter was observed, indicating that they were moderately halotolerent. In our study, Bacillus, Arthrobacter, and Streptomyces showed optimum growth at pH 8.0, and Streptococcus, Staphylococcus, and Micrococcus showed optimum growth at pH 7.0. Only Corynebacterium showed optimum growth at pH 9.0, indicating that it is tolerant of higher pH conditions.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2016
• Tom: 25
• Numer: 4
• Opis fizyczny: p.1721-1727,ref.

Twórcy

autor

Uddin M.N.

• Center for Biotechnology and Microbiology, University of Swat, Pakistan

autor

Ali M.

• Center for Biotechnology and Microbiology, University of Swat, Pakistan

autor

Muhammad M.

• Department of Microbiology, University of Swabi, Pakistan

autor

Farooq M.

• Department of Microbiology, University of Swabi, Pakistan

autor

Ahmad N.

• Center for Biotechnology and Microbiology, University of Swat, Pakistan

autor

Jamil J.

• Department of Microbiology, University of Swabi, Pakistan

autor

Kalsoom J.J.

• Department of Microbiology, University of Swabi, Pakistan

autor

Adnan M.

• Department of Agriculture, University of Swabi, Pakistan

autor

Shah N.

• Department of Zoology, University of Swabi, Pakistan

autor

Khan A.

• Department of Zoology, University of Swabi, Pakistan

Bibliografia

• 1. Okoh A.I. Biodegradation of Bony light crude oil in soil microcosm by some bacterial strains isolated from crude oil flow station saver pits in Nigeria. African Biotech, 2 (5), 104, 2003.
• 2. Energy Information Administration (EIA). (2006). Annual Energy Review. http://www.eia.gov/kids/energy.cfm?page=about_home-basics.
• 3. Sebiomo A., Bankole S.A., Awosanya A.O. Determination of the ability of microorganisms isolated from mechanic soil to utilise lubricating oil as carbon source. African Journal of Microbiology Research, 4 (21), 2257, 2010.
• 4. Surridge A.K.J. Denaturing gradient gel electrophoresis characterisation of microbial communities in polycyclic aromatic hydrocarbon and polychlorinated biphenyl contaminated soil. PhD Thesis. University of Pretoria, South Africa, 1, 2007.
• 5. Sharma S. Bioremediation: Features, Strategies and applications. Asian Journal of Pharmacy and Life Science, 2 (2), 202, 2012.
• 6. Das K., Mukarjee A.K. Crude petroleum oil degradation efficiency of Bacillus Subtitles and Pseudomonas aerations strains isolated from a petroleum oil contaminated soil from North-East India. Bioresearch Technology, 98 (7), 1339,2011.
• 7. Santhini K., Myla J., Sajani S., Usharani G. Screening of micrococcus spp from oil contaminated soil with refrence to bioremediation. Botany Research International, 2 (4), 248, 2009.
• 8. Paramanik D., Rajalakshmi G. Biodegredation of petroleum hydrocarbons pollutants in soil using microbial consortium. International Journal of Plant, Animal and Environmental Sciences, 3 (3), 173, 2013.
• 9. Badrunnisa S., Shantaram M., Pai R.V. Isolation, Characterization and Identification of bacteria from coolant oils. International Journal of Applied Biology and Pharmaceutical Technology. 2 (3), 444, 2011.
• 10. Ma Y., Wang L., Shao Z. Pseudomonas, the dominant polycyclic aromatic hydrocarbon-degrading bacteria isolated from Antarctic soils and the role of large plasmids inhorizontal gene transfer. Environmental Microbiology, 8 (3), 455, 2006.
• 11. Raza C., Liaqat S., Bashir S., Naseer M., Ishrat N. Characterization of crude oil contaminated soil bacteria and laboratory-scale biodegradation experiments. Biologia (Pakistan), 57 (1, 2), 47, 2011.
• 12. Erdogan E.E., Sahin F. Karaca A. Determination of petroleum degrading bacteria isolated from crude oil contaminated soil in Turkey. African Journal of Biotechnology, 10 (20), 166, 2011.
• 13. Mhamane P., Shaikh N., Sohani H.M. Rajashree Isolation and Characterization of Hydrocarbon Degrading Bacteria’s isolated from Diesel Polluted Soil from Various Petrol-Diesel Bunk of Solapur. International Journal of Recent Trends in Science And Technology, 9 (2), 178, 2013.
• 14. Idemudia M.I., Nosagie A.O., Omorede O. Comparative assessment of degradation potentials of bacteria and actinomycetes in soil contaminated with motorcycle spent oil. Asian Journal of Science and Technology, 5 (8), 482, 2014.
• 15. Thenmozhi R., Nagasthya A. Thajuddin N. Studies on bioremedation of used engine oil by consortium cultures. Advances in Environmental Biology, 5 (6), 1051, 2011.
• 16. Dzulkapli N.F., Talib S.A., Ramasamy K., Yang Y.C. Identafication of bacterial strains capable of degrading malasian petroleum sludge. Conference on Scientific and Social Research, 8 (9), 55, 2009.
• 17. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T. Bergey’s Manual of Determinative Bacteriology. 9th Ed. Williams and Wilkins. Mpp: 71-561, 1994.
• 18. Oboh B.O., Ilori M.O., Akinyemi J.O., Adebusoye S.A. Hydrocarbon Degrading Potentials of Bacteria Isolated from a Nigerian Bitumen (Tarsand) Deposit. Nature and Science, 4 (3), 1, 2006.
• 19. Roy A.S, Baruah R., Borah M., Singh A.K., Boruah H.P.D., Saikia N., Deka M., Dutta N., Bora T.C. Bioremediation potential of native hydrocarbon degrading bacterial strains in crude oil contaminated soil under microcosm study. International Biodeterioration & Biodegradation, 94, 79, 2014.
• 20. Panda S.K., Kar R.N., Panda C.R. Isolation and identification of petroleum hydrocarbon degrading microorganisms from oil contaminated environment. International Journal of Environmental Sciences, 3 (5), 1315, 2013.
• 21. Jayashree R., Nithya S.E., Prasanna P.R., Krishnaraju M. Biodegradation capability of bacterial species isolated from oil contaminated soil. J. Acad. Indus. Res, 1 (3), 140, 2012.
• 22. Jaboro A.G., Akortha E.E., Obayagbona O.N. Suspectibility to heavy metals and hydrocarb on clastic attributes of soil microbiota. International Journal of Agriculture Biosciences, 2 (5), 206, 2013.
• 23. Shubhrasekhar C., Supriya M., Karthik L., Gaurav K., Bhaskara Rao K.V. Isolation, characterization, and application of biosurfactant produced by marine action bacteria isolated from saltpan soil from costal area of Andhra Pradesh, India. Research Journal of Biotechnology, 8 (1), 18, 2013.
• 24. Ahirwar S., Dehariya K. Isolation and characterization of hydrocarbon degrading microorganisms from petroleum oil contaminated soil sites.Bulletin of Environmental and Scientific Research, 2 (4), 5, 2013.
• 25. Sunita V.J., Dolly R.P., Bateja S., Vivek U.N. Isolation and Screening for Hydrocarbon Utilizing Bacteria (HUB)from Petroleum Samples. International Journal of Current Microbiology and Applied Sciences, 2 (4), 48, 2013.
• 26. Rehab S., Ramadha Rebah N., Jabbar , Abdulatif N. Isolation, Identification, and Assessment the ability of local Streptomyces isolate from Iraq to utilize crude oil and diesel fuel. Science Park Journals, 2 (1), 9, 2013.
• 27. Mohan T.S., Palavesam A., Immanvel G. Isolation and characterization of lipase-producing Bacillus strains from oil mill waste. African Journal of Biotechnology, 7 (15), 2728, 2008.
• 28. Vishnupriya B., Sundaramoorthi C., Kalaivani M., Selvam K. Production of lipase from Streptomyces griseus and evaluation of Bioparameters. International J. Chem. Tech. Res, 2 (3), 1380, 2010.
• 29. Nisha P., Nayana M., Asokan A. Production of lipase from Micrococcus flavus and influence of bioparameters. IOSR Journal of Pharmacy and Biological Sciences, 9 (2), 67, 2014.
• 30. Olajuyigbe F.M., Ehiosun K.L. Production of thermostable and organic solvent-tolerant alkaline protease from Bacillus coagulans PSB- 07 under different submerged fermentation conditions. African Journal of Biotechnology, 12 (21), 3341, 2013.
• 31. Nisha P., Das A., Saritha K.V. Production of Cellulase from Micrococcus spand effect of growth parameters. International Journal of Pharma Research and Health Sciences, 2 (3), 236, 2014

Identyfikatory

DOI

10.15244/pjoes/61670