Effect of bed rest on the adenine nucleotides concentration in human blood platelets

• Autorzy: Buczynski A. , Kedziora J. , Wachowicz B. , Zolynski K.
• Języki publikacji: EN

Abstrakty

EN

The effect of immobilization in bed on metabolism and function of human blood platelet was studied. Blood platelets taken from patients with bone fractures after long term bed rest (14 days and 28 days) demonstrated significantly reduced concentration of total adenine nucleotides (after 28 days reduction about 30%). This decrease of total platelet adenine nucleotides af er immobilization in bed is probably caused by s imulation of platelet secretory process. Thrombin which released from confrol platele s 58.2%±1.5% of total adenine nucleotides liberated decreased amounfs (only 23.1 %±3.3% of total) of nucleotides from patient platelets isolated after 28 days of immobilization in bed. Loss of nucleotides from platelets was accompanied by sligh ly increased extent of platelet aggregation. It is concluded that during bed rest the reactivity of blood platelets (aggregation and release reaction) is stimulated.

Słowa kluczowe

EN

platelet; patient; bed rest; aggregation; adenine nucleotide; thrombin

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1991
• Tom: 42
• Numer: 4
• Opis fizyczny: p.389-395,fig.,ref.

Twórcy

autor

Buczynski A.

• Department of Physiology, Institute of Fundamental Sciences, Military Medical Academy, Pl. Halera 1, Lodz, Poland

autor

Kedziora J.

• Department of Physiology, Institute of Fundamental Sciences, Military Medical Academy, Pl. Halera 1, Lodz, Poland

autor

Wachowicz B.

• Department of Biochemistry, Institute of Biochemistry, University of Lodz, Lodz, Poland

autor

Zolynski K.

• Department of Traumatic Surgery and Orthopedy, Military Medical Academy, Lodz, Poland

Bibliografia

• 1. Fukami MH, Holmsen H, Salganicoff L. Adenine nucleotides metabolism of blood platelets. IX. Time course of secretion and changes of energy metabolism in thrombin-treated platelets. Biochim Biophys Acta 1976; 74.: 301-305.
• 2. Holmsen H. Energy metabolism and platelet responses. Vox Sang 1981; 40: 1-7.
• 3. Holmsen H, Day HJ. Adenine nucleotides and platelet function. Ser Haematol 1971; 4: 28-58.
• 4. Tkaczewski W, Kędziora J, Buczyński A, Ryniec A, Błaszczyk J, Dziekański S. Blood platelets: aggregation, malonyldialdehyde concentration and SOD-1 activity in patien; s with coronary heart disease after captopril administration. In: Current Advances in ACE Inhibition. A Mac Gregor, PS Sever (eds). Churchill Livingstone 1989; pp. 191-193.
• 5. Buczyński A, Kędziora J. Effect of submaximal physical exercise on oxygen metabolism in blood platelets of healthy men. XXXI Intern Congress Physiol Sciences, Helsinki, 1989.
• 6. Kędziora J, Buczyński A, Kafar K, Żołyński K. Effect of hypokinesis on antioxidative defense in blood platelets. Acta Physiol Pol. 1990; 34; 161-162.
• 7. Buczyński A, Kędziora J, Tkaczewski W. Wachowicz В. Effect of submaximal physical exercise on antioxidative protection of human blood platelets. Intern J Sport Med 1991; 12: 52-54.
• 8. Błaszczyk J, Buczyński A, Kędziora J. Blood platelet and erythrocyte superoxide dismutase (SOD-1) activity and malonyldialdehyde concentration in healthy men following submaximal exercise. Acta Physiol Pol 1987; 38: 56-57.
• 9. Wachowicz В. Adenine nucleotides in thrombocytes of birds. Cell Biochem Fund 1984; 2: 167-170.
• 10. Wachowicz В, Krajewski T. Activation of blood platelets and prostaglandin biosynthesis. Acta Vniversitatis Lodz. 1986; 5: 133-143.
• 11. Wachowicz В, Buczyński A, Krajewski T, Tkaczewski W, Kędziora J. Microtechnique of adenine nucleotides assay in human platelets. Pol Tyg Lek 1988; 7: 230-233.
• 12. Born GVR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962;194:927-928.
• 13. Birkhead NC, Haupt GJ, Mayers RN. Effect of prolonged bed rest on cardio-dynamics. Am J Med Sci 1963; 245: 118-119.
• 14. Ernst E, Schmidt-Pauly E, Muhling P, Matrai A. Blood viscosity in patients with bone fractures and long teim bed rest. Br J Surg 1987; 74: 301-305.
• 15. Greenleaf JE, Bernauer EM, Juhos LT, Young HL, Morse JT, Staley RW. Effects of exercise on fluid exchange and body composition in man during 14 days bed rest. J Appl Physiol Respirat Environ Exercise Physiol 1977; 43: 126-132.