Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 23 | 3 |

Tytuł artykułu

Quality assessment of eggs from laying hens fed a mixture with lucerne protein concentrate

Warianty tytułu

Języki publikacji



The aim of this study, carried out on ISA Brown laying hens, was to determine the effect of dietary lucerne protein concentrate (LPC) on egg quality. The birds were allocated to three experimental groups, 30 birds each. The control group (C) was fed a standard feed mixture without the experimental additive. Diets of hens from groups D-1 and D-2 were supplemented with 1.5% and 3.0% of LPC, respectively, instead of soyabean meal. In the laying period, 40 eggs from each group were collected twice (week 33 and 53 of rearing) for evaluation of selected quality traits: specific weight of egg, eggshell colour, eggshell resistance, eggshell weight, egg yolk weight, egg yolk colour, thick albumen height, albumen quality, eggshell thickness, eggshell compactness (density), meat spots and blood spots. Egg yolks were analysed for contents of fat, total cholesterol and fatty acid composition, as well as for parameters of redox status (superoxide dismutase, catalase, hydrogen peroxides, malondialdehyde). The 1.5% and 3.0% additions of LPC increased the colour intensity of egg yolk, but at the same time, induced lipid peroxidation processes in it. The possible latter effect should be taken into account when intending to increase yolk colouration via LPC dietary supplementation. Our results show that addition of 1.5% LPC can be recommended in feed for laying hens.

Słowa kluczowe








Opis fizyczny



  • Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
  • Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
  • Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
  • Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland


  • Andurand J., Coulmier D., Despres J.L., Rambourg J.C., 2010. Industrial extraction of proteins and pigments from alfalfa: status and prospects (in French). Innov. Agronom. 11, 147–156
  • AOAC, 2011. Association of Official Analytical Chemistry, Official Methods of Analysis. 18th Edition. Gaithersburg, MA
  • Aziz A.B., Grossman S., Budowski I., Ascarielli I., Bondi A., 2006. Antioxidant properties of lucerne extracts. J. Sci. Food Agric. 19, 605–608
  • Blount J.D., Surai P.F., Houston D.C., Møller A.P., 2002. Patterns of yolk enrichment with dietary carotenoids in gulls: the roles of pigment acquisition and utilization. Funct. Ecol. 16, 445–453
  • Bologa M., Pop I.M., Albu A., 2013. Research on chemical composition of chicken egg from different system of production (conventional and organic). Lucrări. Şti. Zoot. 59, 80–85
  • Botsoglou N.A., Fletouris D.J., Papageorgiou G.E., Vassilopoulos V.N., Mantis A.J., Trakatellis A.G., 1994. Rapid sensitive and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food and feed-stuff samples. J. Agr. Food Chem. 42, 1931–1937
  • Claiborne A., 1985. Catalase activity. In: R.A. Greenwald (Editor). Handbook of Methods for Oxygen Free Radical Research. CRC Press, Boca Raton, FL (USA), pp. 283–284
  • Czech A., Ognik K., Grela E.R., 2012. Efficacy of a mixture of synthetic antioxidant and protein xanthophyll alfalfa concentrate in turkey hens feeding. Arch. Geflügelkd. 76, 105–112
  • Davies M.G., Thomas A.J., 1973. An investigation of hydrolytic techniques for the amino acid of foodstuffs. J. Sci. Food Agric. 24, 1525–1540
  • Dong X.F., Gao W.W., Tong J.M., Jia H.Q., Sa R.N., Zhang Q., 2007. Effect of polysavone (alfalfa extract), on abdominal fat deposition and immunity in broiler chickens. Poultry Sci. 86, 1955–1959
  • EFSA, 2009. Opinion on the safety of ‘Lucerne protein concentrate’ as food. EFSA J. 997, 1–19
  • Fernández M., Ordóñez J.A., Cambero I., Santos C., Pin C., De la Hoz L., 2007. Fatty acid compositions of selected varieties of Spanish dry ham related to their nutritional implications. Food Chem. 9, 107–112
  • Folch J., Lees M., Sloane Stanley G.H., 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226, 497–509
  • Franchini A., 2007. Egg quality traits of laying hens reared in organic and conventional systems. Ital. J. Anim. Sci. 6, 728–730
  • Francis G., Kerem Z., Makker H.P.S., Becker K., 2002. The biological action of saponins in animal systems: a review. Brit. J. Nutr. 88, 587–605
  • Fredriksson S., Elwinger K., Pickova J., 2006. Fatty acid and carotenoid composition of egg yolk as an effect of microalgae addition to feed formula for laying hens. Food Chem. 99, 530–537
  • Gay C., Gebicki J.M., 2002. Perchloric acid enhances sensitivity and reproducibility of the ferric-xylenol orange peroxide assay. Anal. Biochem. 304, 42–46
  • Greenwald R.A., 1985. CRC Handbook of Methods for Oxygen Radical Research. CRC Press Boca Raton, FL (USA)
  • Grela E.R., Pietrzak K., Kowalczuk-Vasilev E., Klebaniuk R., Borucka B., 2013. Analysis of chemical composition of protein-xanthophyll concentrate (in Polish). Przem. Chem. 92, 1484–1488
  • Haugh R.R., 1937. The Haugh unit for measuring egg quality. U.S. Egg Poultry Mag. 43, 552–555
  • Kaya S., Erdogan Z., Erdogan S., 2003. Effect of different dietary levels of Yucca schidigera powder on the performance, blood parameters, and egg yolk cholesterol of laying quails. J. Vet. Med. 50, 14–17
  • Khaleel A.E., Gad M.Z., El-Maraghy S.A., Hifnawy M.S., Abdel-Satter E., 2005. Study of hypocholesterolemic and antiatherosclerotic properties of Medicago sativa. J. Food Drug Anal. 13, 212–218
  • Laudadio V., Tufarelli V., 2010. Treated faba bean (Vicia faba var. minor) as substitute for soybean meal in diet of early phase laying hens: egg-laying performance and egg quality. Poultry Sci. 89, 2299–2303
  • Laudadio V., Tufarelli V., 2011. Influence of substituting dietary soyabean meal for dehulled-micronized lupin (Lupinus albus cv. Multitalia) on early phase laying hens production. Livest. Sci. 140, 184–188
  • Mansoub N.H., 2011. Effect of different level of lucerne extract on performance, egg quality and some blood parameters of laying hens. Ann. Biol. Res. 2, 384–388
  • Mielmann A., 2013. The utilisation of lucerne (Medicago sativa). Brit. Food J. 115, 590–600
  • Nakaue H.S., Lowry R.R., Cheeke P.R., Arscott G.H., 1980. The effect of dietary lucerne of varying saponin content on yolk cholesterol level and layer performance. Poultry Sci. 59, 2744–2748
  • NRC, 1994. Nutrient Requirements of Poultry. 9th revised Edition. Washington, DC
  • Nys Y., Sauveur B., 2004. The nutritional value of eggs. INRA Prod. Anim. 17, 385–393
  • Palozza P., Calviello G., Emilia de Leo M., Serini S., Bartoli G.M., 2000. Canthaxanthin supplementation alters antioxidant enzymes and iron concentration in liver of Balb/c mice. J. Nutr. 130, 1303–1308
  • Radwan N.L., Hassan R.A., Qota E.M., Fayek H.M., 2008. Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. Int. J. Poultry Sci. 7, 134–150
  • Rosa A.P., Scher A., Sorbara J.O.B., Boemo L.S., Forglarin J., Londero A., 2012. Effects of canthaxantin on the productive and reproductive performance of broiler breeders. Poultry Sci. 91, 660–666
  • Schram E., Moore S., Bigwood E.J., 1954. Chromatographic determination of cystine as cysteic acid. Biochem. J. 57, 33–37.
  • Sim J.S., Kitts W.D., Bragg D.B., 1984. Effect of dietary saponin on egg cholesterol level and laying hen performance. Can. J. Anim. Sci. 64, 977–984
  • Sobczak J., Marek P., 2012. The influence of green fodder of red canna (Canna × generalis) on the intensity of colour of yolks of food eggs (in Polish). Rocz. Nauk. PTZ 8, 65–72
  • Ulbricht T.L., Southgate D.A.T., 1991. Coronary heart disease: seven dietary factors. Lancet 338, 985–992
  • Washburn K.W., Nix D.F., 1974. A rapid technique for extraction of yolk cholesterol. Poultry Sci. 53, 1118–1122
  • Xie Z., Huang J., Xu X., Jin Z., 2008. Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chem. 111, 370–376
  • Zhang W., Zhang K.Y., Ding X.M., Bai S.P., Hernandez J.M., Yao B.,
  • Zhu Q., 2011. Influence of canthaxantin on broiler breeder reproduction, chick quality, and performance. Poultry Sci. 90, 1516–1522


Rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.