Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay

• Autorzy: Radko L. , Minta M. , Jasik A. , Stypula-Trebas S. , Zmudzki J.
• Języki publikacji: EN

Abstrakty

EN

The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 µg/kg for 17α-ethinyloestradiol (s.c.): 1 µg/kg/day (s.c.) and 40 µg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 µg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.

Słowa kluczowe

EN

golden hamster; Mesocricetus auratus; uterotrophic assay; estrogen; sensitivity; histopathological change

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 4
• Opis fizyczny: p.533-539,fig.,ref.

Twórcy

autor

Radko L.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Minta M.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Jasik A.

• Department of Pathology National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Stypula-Trebas S.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzki J.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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Identyfikatory

DOI

10.1515/bvip-2015-0080