Protective effect of lithium chloride on pulmonary injury caused by Actinobacillus pleuropneumoniae via inhibition of GSK-3β-NF-κB-dependent pathway

• Autorzy: Zhang Y. , Xu W. , Yang T. , Huang F.
• Języki publikacji: EN

Abstrakty

EN

Porcine contagious pleuropneumonia (PCP) is a very serious respiratory disease which is difficult to prevent and treat. In this study, the therapeutic effects of lithium chloride (LiCl) on PCP were examined using a mouse model. A mouse model of PCP was established by intranasal infections with Actinobacillus pleuropneumoniae (App). Histopathological analysis was performed by routine paraffin sections and an H-E staining method. The inflammatory factors, TLR4 and CCL2 were analyzed by qPCR. The expression levels of p-p65 and pGSK-3ß were detected using the Western Blot Method. The death rates, clinical symptoms, lung injuries, and levels of TLR-4, IL-1ß, IL-6, TNF-α, and CCL2 were observed to decrease in the App-infected mice treated with LiCl. It was determined that the LiCl treatments had significantly reduced the mortality of the App-infected cells, as well as the expressions of p-p65 and pGSK-3ß. The results of this study indicated that LiCl could improve the pulmonary injuries of mice caused by App via the inhibition of the GSK-3β-NF-κB-dependent pathways, and may potentially become an effective drug for improving pulmonary injuries caused by PCP.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 1
• Opis fizyczny: p.35-44,fig.,ref.

Twórcy

autor

Zhang Y.

• College of Veterinary Medicine, Hunan Agricultural University, Furong District, Nongda Road, No.1, Changsha 410128, China

autor

Xu W.

• College of Veterinary Medicine, Hunan Agricultural University, Furong District, Nongda Road, No.1, Changsha 410128, China

autor

Yang T.

• College of Veterinary Medicine, Hunan Agricultural University, Furong District, Nongda Road, No.1, Changsha 410128, China

autor

Huang F.

• College of Veterinary Medicine, Hunan Agricultural University, Furong District, Nongda Road, No.1, Changsha 410128, China
• Hunan Engineering Technology Research Center for Veterinary Drugs, Hunan Agricultural University, Furong District, Nongda Road, No.1, Changsha 410128, China

Identyfikatory

DOI

10.24425/pjvs.2022.140838