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2011 | 55 | 4 |
Tytuł artykułu

Determination of amoxicillin in pig plasma by liquid chromatography with fluorescence detection

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The concentrations of amoxicillin in pig plasma were determined by HPLC technique. Separation was achieved on a Lichrocart 250 x 4 mm Purospher column and detection was performed with a fluorescence detector. The mobile phase consisted of a mixture of acetonitrile with buffer solution (0.05 M KH₂PO₄, pH 5.6) delivered by a isocratic programme. For amoxicilin assay, the plasma samples were exposed to reaction with formaldehyde under acidic and heating conditions. The limit of detection and quantification, as well as the accuracy and precision of the method were evaluated from spiked plasma samples at concentration levels ranging from 10 to 500 ng‧mL⁻¹. Recovery of spiked amoxicillin was >91%, with coefficient of variation equals 0.12%. This method offers a rapid, repeatable, and accurate procedure and has been useful for determination of pharmacokinetic parameters of amoxicillin in pigs’ and poultry plasma.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
55
Numer
4
Opis fizyczny
p.771-775,fig.,ref.
Twórcy
autor
  • Department of Veterinary Pharmacy, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
Bibliografia
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  • 7. Dousa M., Hosmanová R.: Rapid determination of amoxicillin in premixes by HPLC. J Pharm Biomed Anal 2005, 37, 373-377.
  • 8. Du X., Li C., Sun H.K., Nightingale C.H., Nicolau D.P.: A sensitive assay of amoxicillin in mouse serum and broncho-alveolar lavage fluid by liquid-liquid extraction and reversed-phase HPLC. J Pharm Biomed Anal 2005, 39, 648-652.
  • 9. Fernandez-Torres R., Consentino M.O., Lopez M.A., Mochon M.C.: Simultaneous determination of 11 antibiotics and their main metabolites from four different groups by reversed-phase high-performance liquid chromatography-diode array-fluorescence (HPLC-DAD-FLD) in human urine samples. Talanta 2010, 81, 871-880.
  • 10. Furusawa N.: Rapid liquid chromatographic determination of residual penicillin G in milk. Fresenius J Anal Chem 2000, 368, 624-626.
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  • 16. Luo W., Ang K., Thomson H.C. Jr.: Rapid method for the determination of ampicillin residues in animal muscle tissues by high-performance liquid chromatography with fluorescence detection. J Chromatogr В Biomed Appl 1997, 694, 401-407.
  • 17. Luo W., Hansen E.B. Jr., Ang C.Y., Deck J., Freeman J.P., Thompson H.C. Jr.: Simultaneous determination of amoxicillin and ampicillin in bovine milk by HPLC with fluorescence detection. J Agric Food Chem 1997, 45, 1264—1268.
  • 18. Pei Q., Yang G.P., Li Z.J., Peng X.D., Fan J.H., Liu Z.Q.: Simultaneous analysis of amoxicillin and sulbactam in human plasma by HPLC-DAD for assessment of bioequivalence. J Chromatogr В Analyt Technol Biomed Life Sci 2011, 879, 2000-2004.
  • 19. Samanidou V.F., Giannakis D.E., Papadaki A.: Development and validation of an HPLC method for the determination of seven penicillin antibiotics in veterinary drugs and bovine blood plasma. J Sep Sci 2009, 32, 1302-1311.
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Uwagi
Rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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