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2015 | 37 | 02 |

Tytuł artykułu

Identification of heat-stable proteins in imbibed Cowpea (Vigna unguiculata) seeds


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Heat-stable protein fraction in seeds is believed to enrich many proteins functioning in the acquisition of stress-tolerance of seeds. In this study, the composition of heat-stable protein fraction in imbibed cowpea (Vigna unguiculata) seeds was analyzed by SDS-PAGE and twodimensional gel electrophoresis coupled with mass spectrometry. The results indicated that approximately 12.4 % of seed soluble proteins were stable after heat treatment at 100 C for 10 min. Twenty-two putative heat-stable proteins were identified using MALDI-TOF/TOF MS. Most of these heat-stable proteins were late embryogenesis abundant proteins, and there were other stress-related proteins including Cu/Zn superoxide dismutase and 17.4 kDa Class I heat-shock protein. A cyclophilin protein, a cleavage and polyadenylation specificity factor and a Pumilio-family RNA binding protein were also present in the heat-stable fraction. The identified heat-stable proteins were more hydrophilic proteins and may accumulate to stabilize cellular components and maintain seed viability during seed development and germination.

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Opis fizyczny

Article: 24 [7 p.], fig.,ref.


  • Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 135 Xin-gang Road, Guangdong 510275, China
  • Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 135 Xin-gang Road, Guangdong 510275, China


  • Abbasi N, Park YI, Choi SB (2011) Pumilio Puf domain RNAbinding proteins in Arabidopsis. Plant Signal Behav 6:364–368
  • Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F, Covarrubias AA (2008) The enigmatic LEA proteins and other hydrophilins. Plant Physiol 148:6–24
  • Boudet J, Buitink J, Hoekstra FA, Rogniaux H, Larré C, Satour P, Leprince O (2006) Comparative analysis of the heat stable proteome of radicles of Medicago truncatula seeds during germination identifies late embryogenesis abundant proteins associated with desiccation tolerance. Plant Physiol 140:1418–1436
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
  • Brini F, Hanin M, Lumbreras V, Amara I, Khoudi H, Hassairi A, Pagés M, Masmoudi M (2007) Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana. Plant Cell Rep 26:2017–2026
  • Brini F, Saibi W, Amara I, Gargouri A, Masmoudi K, Hanin M (2010) Wheat dehydrin DHN-5 exerts a heat prtective effect on bglucosidase and glucose oxidase activities. Biosci Biotech Biochem 74:1050–1054
  • Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, Carnemolla B, Orecchia P, Zardi L, Righetti PG (2004) Blue silver: a very sensitive colloidal CoomassieG-250 staining for proteome analysis. Electrophoresis 25:1327–1333
  • Chatelain E, Hundertmark M, Leprince O, Le Gall S, Satour P, Deligny-penninck S, Rogniaux H, Buttink J (2012) Temporal profiling of the heat-stable proteome during late maturation of Medicago truncatula seeds identifies a restricted subset of late embryogenesis abundant proteins associated with longevity. Plant Cell Enviro 35:1440–1455
  • Diouf D (2011) Recent advances in cowpea [Vigna unguiculata (L.)Walp.] ‘‘omics’’ research for genetic improvement. Afr J Biotechnol 10:2803–2810
  • Fecht-Christoffers MM, Braun HP, Lemaitre-Guillier C, VanDorsselaer A, Horst WJ (2003) Effect of manganese toxicity on the proteome of the leaf apoplast in cowpea. Plant Physiol 133:1935–1946
  • Filippou P, Antoniou C, Fotopoulos V (2011) Effect of drought and rewatering on the cellular status and antioxidant response of Medicago truncatula plants. Plant Signal Behav 6:270–277
  • Huang H, Møller IM, Song SQ (2012) Proteomics of desiccation tolerance during development and germination of maize embryos. J Proteomics 75:1247–1262
  • Huh SU, Kim MJ, Paek KH (2013) Arabidopsis Pumilio protein APUM5 suppresses Cucumber mosaic virus infection via direct binding of viral RNAs. PNAS 110:779–784
  • Ismail AM, Hall AE, Close TJ (1999) Allelic variation of a dehydrin gene cosegregates with chilling tolerance during seedling emergence. PNAS 96:13566–13570
  • Kim HS, Lee JH, Kim JJ, Kim CH, Jun SS, Hong YN (2005) Molecular and functional characterization of CaLEA6, the gene for a hydrophobic LEA protein from Capsicum annuum. Gene 344:115–123
  • Kochhar S, Kochhar VK (2008) Identification and characterization of a super-stable Cu–Zn SOD from leaves of turmeric (Curcuma longa L.). Planta 228:307–318
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
  • Lee JR, Lee SS, Jang HH, Lee YM, Park JH, Park S, Moon JC, Park SK, Kim SY, Lee SY, Chae HB, Jung YJ, Kim WY, Shin MR, Cheong G, Kim MG, Kang KR, Lee KO, Yun D, Lee SY (2009) Heat-shock dependent oligomeric status alters the function of a plant-specific thioredoxin-like protein, AtTDX. PNAS 106:5978–5983
  • Madanala R, Gupta V, Deeba F, Upadhyay SK, Pandey V, Singh PK, Tuli R (2011) A highly stable Cu/Zn superoxide dismutase from Withania somnifera plant: gene cloning, expression and characterization of the recombinant protein. Biotechnol Lett 33:2057–2063
  • NDong C, Danyluk J, Wilson KE, Pocock T, Huner NP, Sarhan F (2002) Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins: molecular characterization and functional analyses. Plant Physiol 129:1368–1381
  • Nogueira FCS, Gonc¸alves EF, Jereissati ES, Santos M, Costa JH, Oliveira-Neto OB, Soares AA, Domont GB, Campos FAP (2007) Proteome analysis of embryogenic cell suspensions of cowpea (Vigna unguiculata). Plant Cell Rep 26:1333–1343
  • Oliveira E, Amara I, Bellido D, Odena MA, Domínguez E, Pagés M, Goday A (2007) LC-MSMS identification of Arabidopsis thaliana heat-stable seed proteins: enriching for LEA-type proteins by acid treatment. J Mass Spectrom 42:1485–1495
  • Park S, Kim J, Lee J, Hwang I, Cheong H, Nah J, Hahm K, Park Y (2009) Antifungal mechanism of a novel antifungal protein from pumpkin rinds against various fungal pathogens. J Agric Food Chem 57:9299–9304
  • Perrocheau L, Rogniaux H, Boivin P, Marion D (2005) Probing heatstable water-soluble proteins from barley to malt and beer. Proteomics 5:2849–2858
  • Prashanth SR, Sadhasivam V, Parida A (2008) Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in indica rice var Pusa Basmati-1 confers abiotic stress tolerance. Transgenic Res 17:281–291
  • Qureshi MI, Qadir S, Zolla L (2007) Proteomics-based dissection of stress-responsive pathways in plants. J Plant Physiol 164:1239–1260
  • Ried JL, Walker-Simmons MK (1990) Synthesis of abscisic acidresponsive, heat-stable proteins in embryonic axes of dormant wheat grain. Plant Physiol 93:662–667
  • Ruan S, Ma H, Wang S, Fu Y, Xin Y, Liu W, Wang F, Tong J, Wang S, Chen H (2011) Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed. BMC Plant Biol 11:34
  • Sabarinath S, Khanna S, Khanna-Chopra R (2009) Purification and characterization of thermostable Cu/Zn superoxide dismutase from Chenopodium murale. Physiol Mol Biol Plants 15:199–209
  • Samarah NH, Mullen RE (2006) Total soluble and dehydrin-like proteins in full-rounded and shriveled seeds of soybean in response to drought stress. J Food Agric Environ 4:260–263
  • Scarpeci TE, Zanor MI, Valle EM (2008) Investigating the role of plant heat shock proteins during oxidative stress. Plant Signal Behav 3:856–857
  • Sharma AD, Vasudeva R, Kaur R (2006) Expression of a boilingstable protein (BsCyp) in response to heat shock, drought and ABA treatments in Sorghum bicolour. Plant Growth Regul 50:249–254
  • Shin SY, Lee HS, Kwon SY, Kwon ST, Kwak SS (2005) Molecular characterization of a cDNA encoding copper/zinc superoxide dismutase from cultured cells of Manihot esculenta. Plant Physiol Biochem 43:55–60
  • Sinniah UR, Ellis RH, John P (1998) Irrigation and seed quality development in rapid-cycling Brassica: soluble carbohydrates and heat-stable proteins. Ann Bot 82:647–655
  • Timko MP, Singh BB (2008) Cowpea, a multifunctional legume. In: Moore PH, Ming R (eds) Genomics of tropical crop plants. Springer Science Business Media, LLC, New York, pp 227–258
  • Vessal S, Siddique KHM, Atkins CA (2012) Comparative proteomic analysis of genotypic variation in germination and early seedling growth of chickpea under suboptimal soil–water conditions. J Proteome Res 11:4289–4307
  • Vicient CM, Hull G, Guilleminot J, Devic M, Delseny M (2000) Differential expression of the Arabidopsis genes coding for Emlike proteins. J Exp Bot 51:1211–1220
  • Wang T, Zhang E, Chen X, Li L, Liang X (2010) Identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut (Arachis hypogaea L). BMC Plant Biol 10:267
  • Yang X, Li J, Li X, She R, Pei Y (2006) Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds. Peptides 27:3122–3128
  • Zhao H, Xing D, Li QQ (2009) Unique features of plant cleavage and polyadenylation specificity factor revealed by proteomic studies. Plant Physiol 151:1546–1556
  • Zhu C, Wang Y, Li Y, Bhatti KH, Tian Y, Wu J (2011) Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection. Plant Physiol Bioch 49:1264–1271

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