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2011 | 67 | 08 |

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Zastosowanie mikrochipu fluidycznego typu Lab-on-Chip do oceny jakości oocytów i zarodków ssaków

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Microfluidic chip system model (Lab-on-chip) in research on quality of mammalian oocytes and embryos

Języki publikacji



The developmental competence of oocytes and embryos is one of the basic factors in determining whether the oocytes will be able to grow and mature and whether the embryos will be able to develop in the preimplantation stage and implant properly. There are several methods of evaluating the developmental competence of oocytes and embryos. The most frequently used include the oocyte maturity test using brilliant cresyl blue (BCB), and molecular analyses, such as genomics, transcriptomics, and proteomics. The Lab-on-Chip (LOC) system is a microcytometer that analyzes the spectrum of light absorption. The analysis of oocyte and embryo competence with this system concerns the thickness and color of the zona pellucida, as well as the color and granularity of the cytoplasm. The microfluidic chip includes two micro fibers and two channels: one leading in and one leading out. The total measuring system comprises a docking station with a chip, micro-spectrophotometer, and laptop. The system can therefore be used under non-laboratory conditions. Analysis with this system is a new and noninvasive method of bovine and porcine oocyte and embryo quality assessment, and may in the future be one of the main tools used in evaluating the developmental competence of these cells. This article presents the possible function and use of a system based on the microfluidic technology. Moreover, the construction and applications of the LOC are discussed, including the possibilities of LOC application in analyses of the developmental competence of bovine and porcine oocytes and embryos.








Opis fizyczny



  • Katedra i Zakład Histologii i Embriologii, Wydział Lekarski II, Uniwersytet Medyczny w Poznaniu, ul.Święcickiego 6, 60-781 Poznań


  • 1.Aebersold R., Mann M.: Mass spectrometry-based proteomics. Nature 2003, 422, 198-207.
  • 2.Albertini D. F., Sanfins A., Combelles C. M.: Origins and manifestations of oocyte maturation competencies. Reprod. Biomed. Online 2003, 6, 410-415.
  • 3.Antosik P., Jaśkowski J. M., Jeziorkowski M., Olechnowicz J.: The influence of vaccum pressure on quality and number of recovered oocytes aspirated from ovarian follicles of swine and cows. Arch. Tierz. 2007, 3, 225-324.
  • 4.Antosik P., Kempisty B., Bukowska D., Jackowska M., Włodarczyk R., Budna J., Brüssow K. P., Lianeri M., Jagodziński P. P., Jaśkowski J. M.: Follicular size is associated with the levels of transcripts and proteins of selected molecules responsible for the fertilization ability of oocytes of puberal gilts. J. Reprod. Dev. 2009, 55, 588-593.
  • 5.Antosik P., Kempisty B., Jackowska M., Bukowska D., Lianeri M., Brüssow K. P., Wozna M., Jaskowski J. M.: The morphology of porcine oocytes is associated with zona pellucida glycoprotein 3 and integrin beta 2protein levels. Vet. Med. 2010, 55, 154-162.
  • 6.Antosik P., Kempisty B., Jackowska M., Bukowska D., Woźna M., Lianeri M., Brüssow K. P., Jaśkowski J. M.: Assessment of transcripts and protein contents contributing to cell cycle control and gap junction connections in morphologically variable groups of porcine cumulus-oocyte complexes. Vet. Med. 2010, 55, 512-521.
  • 7.Bargiel S., Dziuban J., Walczak R., Knapkiewicz P., Nieradko Ł., Grzegorska A., Latecki B.: NIR micro spectrometry of chemically aggressive fluids, Micro Total Analysis Systems. Proc. 11th Internat. Conf. Miniaturized Systems for Chemistry and Life Sciences. Paris 2007, 2, 1664-1666.
  • 8.Bhojwani M., Rudolph E., Kanitz W., Zuehlke H., Schneider F., Tomek W.: Molecular analysis of maturation processes by protein and phosphoprotein profiling during in vitro maturation of bovine oocytes: a proteomic approach. Cloning Stem. Cells 2006, 8, 259-274.
  • 9.Bukowska D., Kempisty B., Antosik P., Jaśkowski J. M., Olechnowicz J.: Selected aspects of canine oocytes maturation, fertilization and embryo development in dogs. Medycyna Wet. 2008, 64, 617-736.
  • 10.Coticchio G., Sereni E., Serrao L., Mazzone S., Iadarola I., Borini A.: What criteria for the definition of oocyte quality? Ann N. Y. Acad Sci. 2004, 1034, 132-1044.
  • 11.Fair T., Carter F., Park S., Evans A. C. O., Lonergan P.: Global gene expression analysis during bovine oocyte in vitro maturation. Theriogenology 2007, 68, 91-97.
  • 12.Gardner D. K., Leese H. J.: Assessment of embryo viability prior to transfer by the non-invasive measurement of glucose uptake. J. Exp. Zool. 1987, 242, 103-105.
  • 13.Goovaerts I. G., Leroy J. L., Jorssen E. P., Bols P. E.: Noninvasive bovine oocyte quality assessment: possibilities of a single oocyte culture. Theriogenology 2010, 74, 1509-1520.
  • 14.Görg A., Weiss W., Dunn M. J.: Current two-dimensional electrophoresis technology for proteomics. Proteomics 2004, 4, 3665-3685.
  • 15.Hahn S., Jackson L. G., Kolla V., Mahyuddin A. P., Choolani M.: Noninvasive prenatal diagnosis of fetal aneuploidies and Mendelian disorders: new innovative strategies. Expert Rev. Mol. Diagn. 2009, 9, 613-621.
  • 16.Hoshi H.: In vitro production of bovine embryos and their application for embryo transfer. Theriogenology 2003, 59, 675-685.
  • 17.Jackowska M., Kempisty B., Antosik P., Bukowska D., Budna J., Lianeri M., Rosińska E., Woźna M., Jagodziński P. P., Jaśkowski J. M.: The morphology of porcine oocytes is associated with zona pellucida glycoprotein transcript contents. Reprod. Biol. 2009, 9, 79-85.
  • 18.Jaśkowski J. M.: Przyżyciowe pozyskiwanie komórek jajowych u krów - czynniki ryzyka. Medycyna Wet. 2001, 51, 792-795.
  • 19.Jaśkowski J. M., Kempisty B., Woźna M., Walczak R., Szczepańska P., Dziuban J., Antosik P.: Wybrane metody oceny kompetencji rozwojowej oraz selekcji oocytów i zarodków bydlęcych. Medycyna Wet. 2010, 66, 740-744.
  • 20.Kastrop P. M., Bevers M. M., Destrée O. H., Kruip T. A.: Protein synthesis and phosphorylation patterns of bovine oocytes maturing in vivo. Mol. Reprod. Dev. 1991, 29, 271-275.
  • 21.Kempisty B., Antosik P., Bukowska D., Jackowska M., Lianeri M., Jaśkowski J. M., Jagodziński P. P.: Analysis of selected transcript levels in porcine spermatozoa, oocytes, zygotes and two-cell stage embryos. Reprod. Fertil. Dev. 2008, 20, 513-518.
  • 22.Kempisty B., Antosik P., Bukowska D., Jackowska M., Lianeri M., Jaśkowski J. M., Jagodziński P. P.: Assessment of zona pellucida glycoprotein and integrin transcript contents in porcine oocytes. Reprod. Biol. 2009, 9, 71-78.
  • 23.Kempisty B., Walczak R., Antosik P., Szczepańska P., Woźna M., Bukowska D., Zaorska K., Dziuban J., Jaśkowski J. M.: Systemy oceny kompetencji rozwojowej oocytów oraz zarodków ssaków oparte na technologii mikrofluidycznej typu Lab-on-Chip. Medycyna Wet. 2011, 67, 25-28.
  • 24.Krisher R. L.: The effect of oocyte quality on development. J. Anim. Sci. 2004, 82, 14-23.
  • 25.Kyogoku H., Ogushi S., Miyano T.: Nucleoli from growing oocytes support the development of enucleolated full-grown oocytes in the pig. Mol. Reprod. Dev. 2010, 77, 167-173.
  • 26.Lasiene K., Vitkus A., Valanciűte A., Lasys V.: Morphological criteria of oocyte quality. Medicina (Kaunas) 2009, 45, 509-515.
  • 27.Loos de F. A., Bevers M. M., Dieleman S. J., Kruip T. A.: Morphology of preovulatory bovine follicles as related to oocyte maturation. Theriogenology 1991, 35, 527-535.
  • 28.Memili E., First N. L.: Zygotic and embryonic gene expression in cow: a review of timing and mechanisms of early gene expression as compared with other species. Zygote 2000, 8, 87-96.
  • 29.Misirlioglu M., Page G. P., Sagirkaya H., Kaya A., Parrish J. J., First N. L., Memili E.: Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos. Proc. Natl. Acad. Sci. USA 2006, 103, 18905-18910.
  • 30.Opiela J., Katska-Ksiazkiewicz L., Lipiński D., Słomski R., Bzowska M., Ryńska B.: Interactions among activity of glucose-6-phosphate dehydrogenase in immature oocytes, expression of apoptosis-related genes Bcl-2 and Bax, and developmental competence following IVP in cattle. Theriogenology 2008, 69, 546-555.
  • 31.Otoi T., Yamamoto K., Koyama N., Tachikawa S., Suzuki T.: Bovine oocyte diameter in relation to developmental competence. Theriogenology 1997, 48, 769-774.
  • 32.Patrizio P., Fragouli E., Bianchi V., Borini A., Wells D.: Molecular methods for selection of the ideal oocyte. Reprod. Biomed. Online 2007, 15, 346-353.
  • 33.Rodrigues B. A., Rodriguez P., Silva A. E., Cavalcante L. F., Feltrin C., Rodrigues J. L.: Preliminary study in immature canine oocytes stained with brilliant cresyl blue and obtained from bitches with low and high progesterone serum profiles. Reprod. Domest. Anim. 2009, 44, 255-258.
  • 34.Rondeau M., Guay P., Goff A. K., Cooke G. M.: Assessment of embryo potential by visual and metabolic evaluation. Theriogenology 1995, 44, 351-366.
  • 35.Shiku H., Shiraishi T., Ohya H., Matsue T., Abe H., Hoshi H., Kobayashi M.: Oxygen consumption of single bovine embryos probed by scanning electrochemical microscopy. Anal. Chem. 2001, 73, 3751-3758.
  • 36.Smith G. D., Takayama S.: Gamete and embryo isolation and culture with microfluidics. Theriogenology 2007, 68, 190-195.
  • 37.Szczepanska P., Walczak R., Dziuban J., Jackowska M., Kempisty B., Jaskowski M. J., Bargiel S.: Lab-on-chip quality classification of porcine/bovine oocytes. Proc. Chemistry 2009, 1, 341-344.
  • 38.Szczepańska P., Walczak R., Dziuban J., Kempisty B., Jackowska M., Antosik P., Jaśkowski J., Bargiel S.: Ocena jakościowa komórek rozrodczych zwierząt hodowlanych z wykorzystaniem mikrocytometru typu lab-chip. Elektronika 2010, 6, 93-96.
  • 39.Walczak R., Szczepańska P., Dziuban J., Kempisty B., Jackowska M., Antosik P., Jaśkowski J. M., Chełmońska-Soyta A. L.: Lab-on-a-chip for developmental competence assessment of bovine oocytes. Elektronika 2010, 11, 30-33.
  • 40.Zeggari R., Wacogne B., Pieralli C., Roux C., Gharbi T.: A full micro-fluidic system for single oocyte manipulation including an optical sensor for cell maturity estimation and fertilization indication. Sensors Actuators 2007, 125, 664-671.

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