Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 57 | 4 |

Tytuł artykułu

Immunomodulatory effect of Mycoplasma bovis in experimentally infected calves

Warianty tytułu

Języki publikacji



The effect of three different field isolates of Mycoplasma bovis on selected immunological parameters in experimentally infected calves was studied. Calves were kept separately in 4 experimental groups, and animals of 3 groups were infected intratracheally with one of the three selected isolates of M. bovis. The control group was inoculated intra-tracheally with sterile physiological saline. Nasal swabs and blood samples were collected just before the calf inoculation, then daily for seven days, and then weekly for another three weeks. The presence of M bovis antigen, M. bovis antibodies, total protein, gamma globulins, IgA, IgM, IgG, acute phase proteins (haptoglobulin and serum amyloid A), as well as interferon-γ and interleukin-4 concentrations were determined. M. bovis was detected intermittently during the study in the infected groups from day 1, whilst the control group remained free of the pathogen. M. bovis antibodies were detected in some of infected animals in the second, third, and fourth week arter infection. The stimulation and/or immunological suppression varied between the M bovis isolates used for the inoculation. All M. bovis isolates induced a rise of APP and gamma globulin concentrations in infected calves. However, in this study the mucosal immune response appeared to be down-regulated, which was expressed with a general lack of IgA stimulation.

Słowa kluczowe








Opis fizyczny



  • Department of Cattle and Sheep Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Department of Cattle and Sheep Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Mycoplasma Group, Animal Health and Veterinary Laboratories Agency (Weybridge), Woodham Lane, Addlestone, Surrey KT15 3NB, U.K.
  • Department of Cattle and Sheep Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland


  • 1. Arcangoli M.A., Duet A., Meyer G., Dernburg A., Bézille P., Pomuarat F., Le Grand D.: The role of Mycoplasma bovis in bovine respiratory disease outbreaks in veal calf feedlots. Vet J 2008, 177, 89-93.
  • 2. Arredouani M.. Matthijs P., Van Hoeyveld E., Kasran A., Baumami H., Ceuppens J.L., Stevens E.: Haptoglobin directly affects T cells and suppresses T helper cell type 2 cytokine release. Immunology 2003, 108, 144-151.
  • 3. Ayling R.D., Bashiruddin S.E., Nicholas R.A.J.: Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec 2004, 155, 413-416.
  • 4. Dudek K., Bednarek D., Szacawa E.: Evaluation of immune response in seropositive cattle for Mycoplasma bovis. Bull Vet Inst Pulawy 2011, 55, 631-634.
  • 5. Dudek K., Bednarek D., Szymańska-Czerwińska M.: Acute phase response in calves as a result of experimental challenge with Mycoplasma bovis. Bull Vet Inst Pulawy 2010, 54, 517-520.
  • 6. Dudek K., Bednarek D.: Last survey of Mycoplasma bovis prevalence in Polish cattle affected with respiratory syndrome. Bull Vet Inst Pulawy 2012, 56, 447-451.
  • 7. El-Ghmati S.M., Arredouani M., Van Hoeyveld E.M., Ceuppens J.L., Stevens E.A.: Haptoglobin interacts with the human mast cell line HMC-1 and inhibits its spontaneous proliferation. Scand J Immunol 2002, 55, 352-358.
  • 8. Ellis J.A.: The immunology of the bovine respiratory disease complex. Vet Clin North Am Food Anim Pract 2001, 17, 535-549.
  • 9. Finch J., Howard C.: Inhibitory effect of Mycoplasma dispar and Mycoplasma bovis on bovine immune responses in vitro. Zentralbl Bakteriol 1990, 20(Suppl.), 563-569.
  • 10. Gatt M.E., Urieli-Shoval S., Preciado-Patt L., Fridkin M., Calco S., Azar Y., Matzner Y.: Effect of serum amyloid A on selected in vitro functions of isolated human neutrophils. J lab Clin Med 1998, 132, 414-420.
  • 11. Houghton S.B., Gourlay R.N.: Synergism between Mycoplasma bovis and Pasteurella haemolytica in calf pneumonia. Vet Rec 1983, 113, 41-42.
  • 12. Jungi T.W., Krampe M., Sileghem M., Griot C., Nicolet J.: Differential and isolate-specific triggering of bovine alveolar macrophage effector functions by mycoplasmas. Microb Pathog 1996, 21, 487-498.
  • 13. Le Grand D., Calavas D., Brank M., Citti C., Rosengarten R., Bézille P., Poumarat F.: Serological prevalence of Mycoplasma bovis infection in suckling beef cattle in France. Vet Rec 2002, 150, 268-273.
  • 14. McAuliffe L., Ellis R., Lawes J., Ayling R.D., Nicholas R.A.J.: 16S rDNA and DGGE: a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol 2005, 54, 1-9.
  • 15. Murata H., Shimada N., Yoshioka M.: Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 2004, 168, 28 -40.
  • 16. Nicholas R., Ayling R., McAuliffe L.: Bovine respiratory disease. In: Mycoplasma diseases of niminants, edited by R.Nicholas. R.Ayling, L.McAuliffe, CABI, Wallingford, 2008, pp. 133-154.
  • 17. Nicholas R., Baker S.: Recovery of mycoplasmas from animals. In: Methods in Molecular Biology. Mycoplasma Protocols, edited by R.Miles, R.Nicholas, Humana Press, Totowa, New Jersey, 1998. pp. 37-43.
  • 18. Nicholas R.A.J., Ayling R.D.: Mycoplasma bovis: disease, diagnosis and control. Res Vet Sci 2003, 74, 105-112.
  • 19. Nicholas R.A.J.: The other M. bovis: Mycoplasma bovis. Cattle Practice 2012, 15, 1-3.
  • 20. Petersen H.H., Nielsen J.P., Heegaard P.M.: Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 2004, 35, 163-187.
  • 21. Radaelli E., Luini M., Loria G.R., Nicholas R.A., Scanziani E.: Bacteriological, serological, pathological and immunohistochemical studies of Mycoplasma bovis respiratory infection in veal calves and adult cattle at slaughter. Res Vet Sci 2008, 85, 282-290.
  • 22. Razin S., Herrmann R.: Molecular biology and pathogenicity of mycoplasmas. Kluwer Academic/Plenum Publishers, New York. 2002, pp. 445-464.
  • 23. Rodríguez F., Bryson D.G., Ball H.J., Forster F.: Pathological and immunohistochemical studies of natural and experimental Mycoplasma bovis pneumonia in calves. J Comp Pathol 1996, 115, 151-162.
  • 24. Rossbacher J., Wagner L., Pasternack M.S.: Inhibitory effect of haptoglobin on granulocyte chemotaxis, phagocytosis and bactericidal activity. Scand J Immunol 1999, 50, 399-404.
  • 25. Soehnlen M.K., Aydin A., Murthy K.S., Lengerich E.J., Hattel A.L., Houser B.A., Fenton G.D., Lysczek H.R., Burns C.M., Townsend A.M., Brooks J.W., Wolfgang D.R., Jayarao B.M.: Epidemiology of Mycoplasma bovis in Pennsylvania veal calves. J Dairy Sci 2012, 95, 247-254.
  • 26. Stefaniak T.: Acute phase proteins in cattle. In: Acute phase proteins in animals, edited by K.Kostro, Z.Gliński, Agricultural University Press, Lublin, 2003, pp. 174-190.
  • 27. Urieli-Shoval S., Linke R.P., Matzner Y.: Expression and function of serum amyloid A, a major acute-phase protein in normal and disease states. Curr Opin Hema 2000, 7, 64-69.
  • 28. Vanden Bush T.J., Rosenbusch R.F.: Characterization of the immune response to Mycoplasma boyis lung infection. Vet Immunol Immunopathol 2003, 94, 23-33.
  • 29. Vanden Bush, T.J., Rosenbusch R.F.: Mycoplasma bovis induces apoptosis of bovine lymphocytes. FEMS Immunol Med Microbiol 2002, 32, 97-103.
  • 30. Xu L., Badolato R.. Murphy W.J., Longo D.L., Anver M., Hale S., Oppenheim J.J., Wang J.M.: A novel biologic function of serum amyloid A. Induction of T lymphocyte migration and adhesion. J Immunol 1995, 155, 1184-1190.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.