PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2016 | 19 | 1 |
Tytuł artykułu

Detection of Helicobacter spp. in the saliva of dogs with gastritis

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to identify the species and determine the prevalence of gastric Helicobacter in the saliva of dogs with gastritis. The study was carried out on 30 dogs of different breeds, genders and ages, which were diagnosed with gastritis. The nested-PCR method was used to detect Helicobacter spp. in saliva. Helicobacter bacteria were found in the saliva samples of 23 (76.6%) dogs. Helicobacter heilmannii was the most commonly detected species of gastric Helicobacter spp. in canine saliva, and was found in 22 (73.3%) cases. The results indicate that gastric Helicobacter spp. occurs relatively frequently in dogs with gastritis. Moreover, the saliva of dogs with gastritis may be a source of Helicobacter spp. infection for humans and other animals. However, further studies are needed to confirm this finding as the PCR method does not distinguish active from inactive infections.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
19
Numer
1
Opis fizyczny
p.133-140,fig.,ref.
Twórcy
autor
  • Department of Internal Diseases with Clinic of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland
autor
  • Department of Internal Diseases with Clinic of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland
autor
  • Department of Internal Diseases with Clinic of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland
  • Department of Internal Diseases with Clinic of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366 Wroclaw, Poland
autor
  • Department of Microbiology, Faculty of Medicine, Wroclaw Medical University, Chalubinskiego 4, 50-368 Wroclaw, Poland
Bibliografia
  • Abdel-Raouf M, Abdel-Gleel Y, Enab A (2014) Study on the Role of Pet Animals for Helicobacter pylori Transmission. J Am Sci 10: 20-28.
  • Agüloğlu S, Turhanoğlu M, Eskimez S, Tacir Y (2006) Detection of helicobacter pylori colonization in human dental plaques and saliva of patients with chronic gastritis. Biotechnol Biotec Eq 20: 173-178.
  • Amorim I, Smet A, Alves O, Teixeira S, Saraiva AL, Taulescu M, Reis C, Haesebrouck F, Gärtner F (2015) Presence and significance of Helicobacter spp. in the gastric mucosa of Portuguese dogs. Gut Pathog 7: 1-8.
  • Bakri MM (2015) Evaluation of non-invasive diagnostic tests for helicobacter pylori infection in symptomatic patients and healthy volunteers. Pak J Physiol 8: 10-12.
  • Bamford KB, Lutton DA, O’Loughlin B, Coulter WA, Collins JS (1998) Nested primers improve sensitivity in the detection of Helicobacter pylori by the polymerase chain reaction. J Infect 36: 105-110.
  • Baele M, Van den Bulck K, Decostere A, Vandamme P, Hänninen ML, Ducatelle R, Haesebrouck F (2004) Multiplex PCR assay for differentiation of Helicobacter felis, H. Bizzozeronii, and H. Salomonis. J Clin Microbiol 42: 1115-1122.
  • Bernander S, Dalen J, Gastrin B, Hedenborg L, Lamke LO, Öhrn R (1993) Absence of Helicobacter pylori in dental plaques in Helicobacter pylori positive dyspeptic patients. Eur J Clin Microbiol Infect Dis 12: 282-285.
  • Cellini L, Grande R, Artese L, Marzio L (2010) Detection of Helicobacter pylori in saliva and esophagus. New Microbiol 33: 351-357.
  • Checchi L, Felice P, Acciardi C, Ricci C, Gatta L, Polacci R, Holton J, Vaira D (2000) Absence of Helicobacter pylori in dental plaque assessed by stool test. Am J Gastroenterol 95: 3005-3006.
  • Chung TH, Kim HD, Lee YS, Hwang CY (2014) Determination of the Prevalence of Helicobacter heilmannii-Like Organisms Type 2 (HHLO-2) Infection in Humans and Dogs Using Non-Invasive Genus/Species-Specific PCR in Korea. J Vet Med Sci 76: 73-79.
  • Clayton CL, Kleanthous H, Coates PJ, Morgan DD, Tabaqchali S (1992) Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J Clin Microbiol 30: 192-200.
  • Diker KS, Haziroglu R, Akan M, Celik S, Kabakc¸i N (2002) The Prevalence, Colonization Sites and Pathological Effects of Gastric Helicobacters in Dogs. Turk J Vet Anim Sci 26: 345-351.
  • Dowsett SA, Kowolik MJ (2003) Oral Helicobacter pylori: can we stomach it? Crit Rev Oral Biol Med 14: 226-233.
  • Eaton KA, Dewhirst FE, Paster BJ, Tzellas N, Coleman BE, Paola J, Sherding R (1996) Prevalence and Varieties of Helicobacter Species in Dogs from Random Sources and Pet Dogs: animal and Public Health Implications. J Clin Microbiol 34: 3165-3170.
  • Ekman E, Fredriksson M, Trowald-Wigh G (2013) Helicobacter spp. in the saliva, stomach, duodenum and faeces of colony dogs. Vet J 195: 127-129.
  • Farrugia A, Keyser C, Ludes B (2010) Efficiency evaluation of a DNA extraction and purification protocol on archival formalin-fixed and paraffin-embedded tissue. Forensic Sci Int 194: e25-e28.
  • Ghil HM, Yoo JH, Jung WS, Chung TH, Youn HY, Hwang CY (2009) Survey of Helicobacter infection in domestic and feral cats in Korea. J Vet Sci 10: 67-72.
  • Henry GA, Long PH, Burns JL, Charbonneau DL (1987) Gastric spirillosis in beagles. Am J Vet Res 48: 831-836.
  • Hong S, Chung Y, Kang WG, Choi YS, Kim O (2015) Comparison of three diagnostic assays for the identification of Helicobacter spp. in laboratory dogs. Lab Anim Res 31: 86-92.
  • Hwang CY, Han HR, Youn HY (2002) Prevalence and Clinical Characterization of Gastric Helicobacter Species Infection of Dogs and Cats in Korea. J Vet Sci 3: 123-133.
  • Jalava K, Kaartinen M, Utriainen M, Happonen I, Hanninen ML (1997) Helicobacter salomonis sp. nov., a Canine Gastric Helicobacter sp. Related to Helicobacter felis and Helicobacter bizzozeronii. Int J Syst Bacteriol 47: 975-982.
  • Jian W, Zhu L, Dong X (2001) New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 51: 1633-1638.
  • Jankowski M, Spużak J, Kubiak K, Glińska-Suchocka K, Biernat M, Kiełbowicz Z (2015) Risk Factors of Gastric Ulcers in Dogs. Pak Vet J 35: 93-97.
  • Kilmartin CM (2002) Dental Implications of Helicobacter pylori. J Can Dent Assoc 68: 489-493.
  • Kubiak K (2006) Kolonizacja błony śluzowej żołądka psów i kotów drobnoustrojami z rodzaju Helicobacter – aspekt kliniczny, 1st ed., Zeszyty Naukowe Akademii Rolniczej we Wrocławiu, Wrocław.
  • Lu JJ, Perng CL, Shyu RY, Chen CH, Lou Q, Chong SK, Lee CH (1999) Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues. J Clin Microbiol 37: 772-774.
  • Majmudar P, Shah SM, Dhunjibhoy KR, Desai HG (1990) Isolation of Helicobacter pylori from dental plaques in healthy volunteers. Indian J Gastroenterol 9: 271-272.
  • Mikkonen TP, Kärenlampi RI, Hänninen ML (2004) Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 54: 753-758.
  • Neiger R, Dieterich C, Burnens A, Waldvogel A, Corthesy-Theulaz I, Halter F, Lauterburg B, Schmassmann A (1998) Detection and prevalence of Helicobacter infection in pet cats. J Clin Microbiol 36: 634-637.
  • Neiger R, Tschudi ME, Burnens A, Göke B, Schmassmann A (1999) Diagnosis and Identification of Gastric Helicobacter Species by Polymerase Chain Reaction in Dogs. Microb Ecol Health Dis 11: 234-240.
  • Priestnall SL, Wiinberg B, Spohr A, Neuhaus B, Kuffer M, Wiedmann M, Simpson KW (2004) Evaluation of „Helicobacter heilmannii” Subtypes in the Gastric Mucosas of Cats and Dogs. J Clin Microbiol 42: 2144-2151.
  • Recordati C, Gualdi V, Tosi S, Facchini RV, Pengo G, Luini M, Simpson KW, Scanziani E (2007) Detection of Helicobacter spp. DNA in the oral cavity of dogs. Vet Microbiol 119: 346-351.
  • Savoldi E, Marinone MG, Negrini R, Facchinetti D, Lanzini A, Sapelli PL (1998) Absence of Helicobacter pylori in dental plaque determined by immunoperoxidase. Helicobacter 3: 283-287.
  • Shojaee Tabrizi A, Jamshidi S, Oghalaei A, Zahraei Salehi T, Bayati Eshkaftaki A, Mohammadi M (2010) Identification of Helicobacter spp. in oral secretions vs. gastric mucosa of stray cats. Vet Microbiol 140: 142-146.
  • Singh V, Mishra S, Rao GR, Jain AK, Dixit VK, Gulati AK, Mahajan D, McClelland M, Nath G (2008) Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60. Helicobacter 13: 30-34.
  • Sjödin S, Trowald-Wigh G, FredrikssonM (2011) Identification of Helicobacter DNA in feline pancreas, liver, stomach, and duodenum: comparison between findings in fresh and formalin-fixed paraffin-embedded tissue samples. Res Vet Sci 91: e28-30.
  • Song Q, Haller B, Ulrich D, Wichelhaus A, Adler G, Bode G (2000) Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction. J Clin Pathol 53: 218-222.
  • Sowjanya K, Maradey-Romero C, Fass R (2013) Diagnostics Tests for Helicobacter pylori. Gastroenterology Endoscopy News 64: 1-8.
  • Swora E, Stankowiak-Kulpa H, Marcinkowska E, Grzymisawski M (2009) Clinical aspects of diagnostics in Helicobacter pylori infection. Nowiny Lekarskie 78: 228-230.
  • Urban J (2010) Helicobacter pylori – Diagnostic Methods and Theraphy. Dent Med Probl 47: 487-495.
  • Van den Bulck K, Decostere A, Baele M, Driessen A, Debongnie JC, Burette A, Stolte M, Ducatelle R, Haesebrouck F (2005) Identification of non-Helicobacter pylori spiral organisms in gastric samples from humans, dogs and cats. J Clin Microbiol 43: 2256-2260.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-0613705d-841d-473a-b8f3-64cf9d78642e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.