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2012 | 34 | 4 |

Tytuł artykułu

Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants

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Nothapodytes nimmoniana (Icacinaceae) yields camptothecin (isoquinoline alkaloid) which is a potent anticancer drug. The major objectives of the present study were to develop an efficient protocol for mass propagation of N. nimmoniana using liquid medium and to compare regeneration with semisolid cultures; as also to quantify the amount of camptothecin in regenerated plants. Adventitious shoots were induced from the callus derived from nodal explants on semisolid and liquid Murashige and Skoog (MS) medium supplemented with 1.0, 2.0, 5.0 and 10.0 µM 6-benzylaminopurine or kinetin or 2-isopentenyl adenine (2-iP). The highest number of adventitious shoots was regenerated on medium supplemented with 2.0 µM BAP. Compared to semisolid medium (41.9 shoots per explant), liquid medium (165.9 shoots per explant) was found suitable for shoot induction and shoot multiplication. Shoots were rooted on MS semisolid medium of one-fourth strength containing IBA (2.4 µM) and IAA (5.7 µM). The plantlets were acclimatized in a growth chamber at 25°C, 60% relative humidity, with 16-h photoperiod (40 µmol m⁻² s⁻¹). The camptothecin content was determined in ex vitro plants using HPLC. The analysis revealed that the leaves and stems of ex vitro plants had a considerable amount of camptothecin and these plants could be used as a raw material for camptothecin extraction.

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  • Department of Botany, Karnatak University, 580003 Dharwad, India
  • Department of Botany, Karnatak University, 580003 Dharwad, India


  • Ciddi V, Shuler ML (2000) Camptothecin from callus culture of Nothapodytes foetida. Biotechnol Lett 22:129–132
  • Fulzele DP, Satdive RK (2003) Somatic embryogenesis, and the evaluation of camptothecin content in Nothapodytes foetida. In Vitro Cell Dev Biol Plant 39:212–216
  • Ilan A, Ziv M, Halvey AH (1995) Propagation and corm development of Brodiaea in liquid cultures. Sci Hortic 63:101–112
  • Jo UA, Murthy HN, Hahn EJ, Paek KY (2007) Micropropagation of Alocasia amazonica using semisolid and liquid cultures. In Vitro Cell Dev Biol Plant 44:26–32
  • Kim EK, Hahn EJ, Murthy HN, Paek KY (2004) Enhanced shoot and bulblet proliferation of garlic (Allium sativum L.) in bioreactor system. J Hortic Sci Biotechnol 79:818–822
  • Lilenbaum RC, Ratain MJ, Miller AA, Hargis JB, Hollis DR, Rosner GL, O’Brien SM, Brewster L, Green MR, Schilsky RL (1995) Phase I study of paclitaxel and topotecan in patients with advanced tumors: a cancer and leukemia group B study. J Clin Oncol 13:2230–2237
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Padmanabha BV, Chandrashekar M, Ramesha BT, Hombe Gowda HC, Gunaga RP, Suhas S, Vasudeva R, Ganeshaiah KN, Uma Shaanker R (2006) Patterns of accumulation of camptothecin, an anti-cancer alkaloid in Nothapodytes nimmoniana Graham., in the Western Ghats, India: implications for identifying highyielding sources of the alkaloid. Curr Sci 90:95–100
  • Park SY, Murthy HN, Paek KY (2000) Mass multiplication of protocorm like bodies (PLBs) using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tissue Organ Cult 63:67–72
  • Praveen N, Naik PMM, Manohar SH, Nayeem A, Murthy HN (2009) In vitro regeneration of brahmi shoot using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol Plant 31:723–728
  • Rai RV (2002) Rapid clonal propagation of Nothapodytes foetida (Wight) Sleumer—a threatened medicinal tree. In Vitro Cell Dev Biol Plant 38:347–351
  • Roja M, Heble MR (1994) The quinoline alkaloids camptothecin and 9-methoxycamptothecin from tissue cultures and mature trees of Nothapodytes foetida. Phytochemistry 36:65–66
  • Romanelli SP, Perego G, Pratesi N, Carenini M, Tortoreto Zunino F (1998) In vitro and in vivo interaction between cisplatin and topotecan in ovarian carcinoma systems. Cancer Chemother Pharmacol 41:385–390
  • Suhas S, Ramesha BT, Ravikanth G, Gunaga RP, Vasudeva R, Ganeshaiah KN, Uma Shaanker R (2007) Chemical profiling of Nothapodytes nimmoniana populations in the Western Ghats, India for anti-cancer compound, camptothecin. Curr Sci 92:1142–1147
  • Thengane SR, Kulkarni DK, Shrikhande VA, Krishnamurthy KV (2001) Effect of thidiazuron on adventitious shoot regeneration from seedling explants of Nothapodytes foetida. In Vitro Cell Dev Biol Plant 37:206–210
  • Thengane SR, Kulkarni DK, Shrikhande VA, Joshi SP, Sonawane KB, Krishamurthy KV (2003) Influence of medium composition on callus induction and camptothecin(s) accumulation in Nothapodytes foetida. Plant Cell Tissue Org Cult 72:247–251
  • Vladu B, Woynarowski JM, Manikumar G, Wani MC, Von Hoff DD, Wadkins RM (2000) 7- and 10-substituted camptothecins: dependence of topoisomerase I-DNA cleavable complex formation and stability on the 7- and 10-substituents. Mol Pharmacol 57:243–251


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